##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII BalI BamHI BcgI
BciVI BglI BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BssHII BstBI Bsu36I
CelII ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV FseI
FspI KpnI KspI MluI MluNI
MroI MscI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SnaBI Sse8387I StuI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 434 GTATAC GT^[AC][GT]AC
AflII 651 CTTAAG C^TTAAG
AflIII 3020 ACGTGT A^C[GA][CT]GT
AlwI 1968 GGATC GGATC(4/5)
Asp700I 1242 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1337 CCTAGG C^CTAGG
BbrPI 3019 CACGTG CAC^GTG
BclI 2584 TGATCA T^GATCA
BfrI 651 CTTAAG C^TTAAG
BlnI 1337 CCTAGG C^CTAGG
BsaAI 3019 CACGTG [CT]AC^GT[GA]
BsaBI 2162 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2788 GAGACC GGTCTC(1/5)
BsmI 1491 GCATTC GAATGC(1/-1)
BspHI 2494 TCATGA T^CATGA
BsrBI 51 GAGCGG CCGCTC(-3/-3)
BsrFI 21 GCCGGC [GA]^CCGG[CT]
Bst1107I 434 GTATAC GTA^TAC
BstEII 1562 GGTTACC G^GT[ACGT]ACC
BstPI 1562 GGTTACC G^GT[ACGT]ACC
BstZ17I 434 GTATAC GTA^TAC
Cfr10I 21 GCCGGC [GA]^CCGG[CT]
EarI 1653 GAAGAG CTCTTC(1/4)
EcoO65I 1562 GGTTACC G^GT[ACGT]ACC
EcoT14I 1337 CCTAGG C^C[AT][AT]GG
FbaI 2584 TGATCA T^GATCA
HindIII 1822 AAGCTT A^AGCTT
Ksp632I 1653 GAAGAG CTCTTC(1/4)
MamI 2162 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1393 CAATTG C^AATTG
MunI 1393 CAATTG C^AATTG
NaeI 21 GCCGGC GCC^GGC
NgoMIV 21 GCCGGC G^CCGGC
PflMI 2801 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3019 CACGTG CAC^GTG
PmlI 3019 CACGTG CAC^GTG
RcaI 2494 TCATGA T^CATGA
SacI 580 GAGCTC GAGCT^C
ScaI 1083 AGTACT AGT^ACT
SphI 2931 GCATGC GCATG^C
SspI 2620 AATATT AAT^ATT
StyI 1337 CCTAGG C^C[AT][AT]GG
Van91I 2801 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1320 TCTAGA T^CTAGA
XmnI 1242 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 106 CGCG CG^CG
129 CGCG
ApaI 123 GGGCCC GGGCC^C
1004 GGGCCC
BanI 56 GGCGCC G^G[CT][GA]CC
164 GGCGCC
BbeI 56 GGCGCC GGCGC^C
164 GGCGCC
BbvCI 97 GCTGAGG CCTCAGC(-5/-2)
2922 GCTGAGG
BglII 397 AGATCT A^GATCT
1886 AGATCT
BsgI 1440 GTGCAG GTGCAG(16/14)
2205 GTGCAG
Bsp1407I 953 TGTACA T^GTACA
3102 TGTACA
BspMI 428 GCAGGT ACCTGC(4/8)
1217 GCAGGT
BsrDI 331 CATTGC GCAATG(2/0)
189 GCAATG
BsrGI 953 TGTACA T^GTACA
3102 TGTACA
BssSI 1179 CACGAG CACGAG(-5/-1)
2995 CACGAG
BstAPI 1442 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2681 GCAGGGTCTGC
BstUI 106 CGCG CG^CG
129 CGCG
BstXI 460 CCAGATACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1753 CCAAGAGACTGG
BstYI 397 AGATCT [GA]^GATC[CT]
1886 AGATCT
DraI 890 TTTAAA TTT^AAA
2779 TTTAAA
EaeI 108 CGGCCA [CT]^GGCC[GA]
2479 CGGCCA
EcoT22I 991 ATGCAT ATGCA^T
2594 ATGCAT
HpaI 1013 GTTAAC GTT^AAC
2969 GTTAAC
KasI 56 GGCGCC G^GCGCC
164 GGCGCC
MflI 397 AGATCT [GA]^GATC[CT]
1886 AGATCT
MslI 1204 CACGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2495 CATGAAAATG
MvnI 106 CGCG CG^CG
129 CGCG
NarI 56 GGCGCC GG^CGCC
164 GGCGCC
NsiI 991 ATGCAT ATGCA^T
2594 ATGCAT
NspI 1303 GCATGT [GA]CATG^[CT]
2931 GCATGC
PvuII 2805 CAGCTG CAG^CTG
2974 CAGCTG
SfoI 56 GGCGCC GGC^GCC
164 GGCGCC
SmaI 90 CCCGGG CCC^GGG
526 CCCGGG
SpeI 501 ACTAGT A^CTAGT
2108 ACTAGT
SspBI 953 TGTACA T^GTACA
3102 TGTACA
XhoII 397 AGATCT [GA]^GATC[CT]
1886 AGATCT
XmaCI 90 CCCGGG C^CCGGG
526 CCCGGG
XmaI 90 CCCGGG C^CCGGG
526 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI