##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AgeI AscI Asp700I
AspI AviII BbrPI BbvCI BcgI
BclI BglI BglII BsaBI BsaWI
BseAI BsiEI BspDI BspEI BsrBI
BssHII BstBI ClaI CpoI EagI
EclXI Eco52I EcoRV EcoT22I FbaI
FseI FspI KspI MamI MluI
MroI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 557 GGTACC G^GTACC
AccII 31 CGCG CG^CG
AclI 4265 AACGTT AA^CGTT
Alw44I 4503 GTGCAC G^TGCAC
Aor51HI 344 AGCGCT AGC^GCT
ApaLI 4503 GTGCAC G^TGCAC
AseI 5901 ATTAAT AT^TAAT
AsnI 5901 ATTAAT AT^TAAT
Asp718I 557 GGTACC G^GTACC
AvrII 4568 CCTAGG C^CTAGG
BamHI 3959 GGATCC G^GATCC
BbeI 106 GGCGCC GGCGC^C
BlnI 4568 CCTAGG C^CTAGG
BlpI 3241 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3241 GCTGAGC GC^T[ACGT]AGC
BsaAI 84 CACGTA [CT]AC^GT[GA]
BsiWI 10 CGTACG C^GTACG
BsmI 1618 GAATGC GAATGC(1/-1)
BsrFI 2091 ACCGGC [GA]^CCGG[CT]
Bst1107I 4370 GTATAC GTA^TAC
BstEII 1313 GGTCACC G^GT[ACGT]ACC
BstPI 1313 GGTCACC G^GT[ACGT]ACC
BstUI 31 CGCG CG^CG
BstZ17I 4370 GTATAC GTA^TAC
CelII 3241 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2091 ACCGGC [GA]^CCGG[CT]
DraIII 4478 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2047 GACTTGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 344 AGCGCT AGC^GCT
EcoO65I 1313 GGTCACC G^GT[ACGT]ACC
EcoRI 2317 GAATTC G^AATTC
HindIII 3362 AAGCTT A^AGCTT
HpaI 1111 GTTAAC GTT^AAC
KasI 106 GGCGCC G^GCGCC
KpnI 557 GGTACC GGTAC^C
MvnI 31 CGCG CG^CG
NarI 106 GGCGCC GG^CGCC
NheI 4318 GCTAGC G^CTAGC
PaeR7I 4174 CTCGAG C^TCGAG
PshBI 5901 ATTAAT AT^TAAT
Psp1406I 4265 AACGTT AA^CGTT
SacI 2103 GAGCTC GAGCT^C
SapI 5138 GAAGAGC GCTCTTC(1/4)
SexAI 1598 ACCAGGT A^CC[AT]GGT
SfoI 106 GGCGCC GGC^GCC
SmaI 2396 CCCGGG CCC^GGG
XhoI 4174 CTCGAG C^TCGAG
XmaCI 2396 CCCGGG C^CCGGG
XmaI 2396 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 106 GGCGCC G[GA]^CG[CT]C
189 GACGCC
AflII 3161 CTTAAG C^TTAAG
5218 CTTAAG
AocI 315 CCTCAGG CC^T[ACGT]AGG
5864 CCTAAGG
AvaI 2396 CCCGGG C^[CT]CG[GA]G
4174 CTCGAG
BciVI 4879 GGATAC GTATCC(6/5)
5591 GGATAC
BfrI 3161 CTTAAG C^TTAAG
5218 CTTAAG
BsaHI 106 GGCGCC G[GA]^CG[CT]C
189 GACGCC
BsmBI 3498 CGTCTC CGTCTC(1/5)
250 GAGACG
BsoBI 2396 CCCGGG C^[CT]CG[GA]G
4174 CTCGAG
BspHI 65 TCATGA T^CATGA
170 TCATGA
BssSI 3467 CACGAG CACGAG(-5/-1)
3648 CACGAG
BstAPI 930 GCAATTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3653 GCAGTCTCTGC
Bsu36I 315 CCTCAGG CC^T[ACGT]AGG
5864 CCTAAGG
Eco81I 315 CCTCAGG CC^T[ACGT]AGG
5864 CCTAAGG
HgaI 189 GACGC GACGC(5/10)
3055 GCGTC
Hin1I 106 GGCGCC G[GA]^CG[CT]C
189 GACGCC
PshAI 3492 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3519 GACTATAGTC
PvuII 640 CAGCTG CAG^CTG
4736 CAGCTG
RcaI 65 TCATGA T^CATGA
170 TCATGA
XcmI 1329 CCACAGACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3325 CCAAAGAGAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI ApaI
ApoI AspEI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI Eam1105I
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SpeI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoII