################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Alw44I Aor51HI ApaLI AscI Asp718I AviII BanI BbeI BbrPI BfrI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BssHII BstEII BstPI ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I FseI FspI KasI KpnI KspI MfeI MluI MroI MunI NarI NdeI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SmaI SpeI Sse8387I SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 1446 CGCG CG^CG AcyI 60 GGCGTC G[GA]^CG[CT]C AhdI 301 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2689 CCTAAGG CC^T[ACGT]AGG AspEI 301 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3245 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1159 CTCGGG C^[CT]CG[GA]G BalI 2501 TGGCCA TGG^CCA BamHI 3176 GGATCC G^GATCC BciVI 642 GGATAC GTATCC(6/5) BglII 1884 AGATCT A^GATCT BsaAI 457 TACGTA [CT]AC^GT[GA] BsaHI 60 GGCGTC G[GA]^CG[CT]C BsoBI 1159 CTCGGG C^[CT]CG[GA]G Bst1107I 4685 GTATAC GTA^TAC BstAPI 3509 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3838 TTCGAA TT^CGAA BstUI 1446 CGCG CG^CG BstXI 3498 CCATCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 4685 GTATAC GTA^TAC Bsu36I 2689 CCTAAGG CC^T[ACGT]AGG DraIII 4822 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 301 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1064 GAAGAG CTCTTC(1/4) Eco81I 2689 CCTAAGG CC^T[ACGT]AGG EcoRI 4085 GAATTC G^AATTC EcoRV 144 GATATC GAT^ATC EcoT22I 13 ATGCAT ATGCA^T FauI 4539 GCGGG CCCGC(4/6) HgaI 61 GCGTC GACGC(5/10) Hin1I 60 GGCGTC G[GA]^CG[CT]C HpaI 3185 GTTAAC GTT^AAC Ksp632I 1064 GAAGAG CTCTTC(1/4) MluNI 2501 TGGCCA TGG^CCA MscI 2501 TGGCCA TGG^CCA MvnI 1446 CGCG CG^CG NaeI 829 GCCGGC GCC^GGC NgoMIV 829 GCCGGC G^CCGGC NheI 3951 GCTAGC G^CTAGC NsiI 13 ATGCAT ATGCA^T NspV 3838 TTCGAA TT^CGAA PflFI 3245 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC ScaI 3895 AGTACT AGT^ACT SfuI 3838 TTCGAA TT^CGAA SgrAI 1492 CACCGGCG C[GA]^CCGG[CT]G SnaBI 457 TACGTA TAC^GTA StuI 4787 AGGCCT AGG^CCT Tth111I 3245 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 1656 GTCTAC GT^[AC][GT]AC 4685 GTATAC ApaI 3003 GGGCCC GGGCC^C 3493 GGGCCC AseI 1973 ATTAAT AT^TAAT 4665 ATTAAT AsnI 1973 ATTAAT AT^TAAT 4665 ATTAAT Asp700I 360 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3652 GAAGGTGTTC AvrII 2578 CCTAGG C^CTAGG 4790 CCTAGG BbvCI 1811 CCTCAGC CCTCAGC(-5/-2) 2653 GCTGAGG BclI 1209 TGATCA T^GATCA 2371 TGATCA BglI 2165 GCCGAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2631 GCCGTGCTGGC BlnI 2578 CCTAGG C^CTAGG 4790 CCTAGG BlpI 191 GCTGAGC GC^T[ACGT]AGC 3405 GCTAAGC BpmI 208 CTCCAG CTGGAG(16/14) 792 CTCCAG Bpu1102I 191 GCTGAGC GC^T[ACGT]AGC 3405 GCTAAGC BsaWI 521 TCCGGT [AT]^CCGG[AT] 1668 TCCGGT BsgI 228 CTGCAC GTGCAG(16/14) 730 CTGCAC BspHI 1100 TCATGA T^CATGA 4745 TCATGA BssSI 593 CACGAG CACGAG(-5/-1) 3563 CTCGTG CelII 191 GCTGAGC GC^T[ACGT]AGC 3405 GCTAAGC EaeI 932 TGGCCG [CT]^GGCC[GA] 2501 TGGCCA FbaI 1209 TGATCA T^GATCA 2371 TGATCA HindIII 2853 AAGCTT A^AGCTT 2942 AAGCTT NcoI 2967 CCATGG C^CATGG 3172 CCATGG PleI 1215 GACTC GAGTC(4/5) 470 GAGTC PshBI 1973 ATTAAT AT^TAAT 4665 ATTAAT PstI 555 CTGCAG CTGCA^G 1469 CTGCAG RcaI 1100 TCATGA T^CATGA 4745 TCATGA SexAI 302 ACCTGGT A^CC[AT]GGT 2115 ACCAGGT SphI 770 GCATGC GCATG^C 1260 GCATGC SspI 2304 AATATT AAT^ATT 2360 AATATT XcmI 2159 CCAGGAGCCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3819 CCAGTTATCAGATGG XmnI 360 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3652 GAAGGTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BcgI BcnI BfaI BmyI BpuAI BsaBI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII