##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
Asp718I AviII BanI BbeI BbrPI
BfrI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BstEII
BstPI ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I FseI
FspI KasI KpnI KspI MfeI
MluI MroI MunI NarI NdeI
NotI NruI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SmaI SpeI Sse8387I SwaI Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 1446 CGCG CG^CG
AcyI 60 GGCGTC G[GA]^CG[CT]C
AhdI 301 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2689 CCTAAGG CC^T[ACGT]AGG
AspEI 301 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3245 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1159 CTCGGG C^[CT]CG[GA]G
BalI 2501 TGGCCA TGG^CCA
BamHI 3176 GGATCC G^GATCC
BciVI 642 GGATAC GTATCC(6/5)
BglII 1884 AGATCT A^GATCT
BsaAI 457 TACGTA [CT]AC^GT[GA]
BsaHI 60 GGCGTC G[GA]^CG[CT]C
BsoBI 1159 CTCGGG C^[CT]CG[GA]G
Bst1107I 4685 GTATAC GTA^TAC
BstAPI 3509 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3838 TTCGAA TT^CGAA
BstUI 1446 CGCG CG^CG
BstXI 3498 CCATCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4685 GTATAC GTA^TAC
Bsu36I 2689 CCTAAGG CC^T[ACGT]AGG
DraIII 4822 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 301 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1064 GAAGAG CTCTTC(1/4)
Eco81I 2689 CCTAAGG CC^T[ACGT]AGG
EcoRI 4085 GAATTC G^AATTC
EcoRV 144 GATATC GAT^ATC
EcoT22I 13 ATGCAT ATGCA^T
FauI 4539 GCGGG CCCGC(4/6)
HgaI 61 GCGTC GACGC(5/10)
Hin1I 60 GGCGTC G[GA]^CG[CT]C
HpaI 3185 GTTAAC GTT^AAC
Ksp632I 1064 GAAGAG CTCTTC(1/4)
MluNI 2501 TGGCCA TGG^CCA
MscI 2501 TGGCCA TGG^CCA
MvnI 1446 CGCG CG^CG
NaeI 829 GCCGGC GCC^GGC
NgoMIV 829 GCCGGC G^CCGGC
NheI 3951 GCTAGC G^CTAGC
NsiI 13 ATGCAT ATGCA^T
NspV 3838 TTCGAA TT^CGAA
PflFI 3245 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 3895 AGTACT AGT^ACT
SfuI 3838 TTCGAA TT^CGAA
SgrAI 1492 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 457 TACGTA TAC^GTA
StuI 4787 AGGCCT AGG^CCT
Tth111I 3245 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1656 GTCTAC GT^[AC][GT]AC
4685 GTATAC
ApaI 3003 GGGCCC GGGCC^C
3493 GGGCCC
AseI 1973 ATTAAT AT^TAAT
4665 ATTAAT
AsnI 1973 ATTAAT AT^TAAT
4665 ATTAAT
Asp700I 360 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3652 GAAGGTGTTC
AvrII 2578 CCTAGG C^CTAGG
4790 CCTAGG
BbvCI 1811 CCTCAGC CCTCAGC(-5/-2)
2653 GCTGAGG
BclI 1209 TGATCA T^GATCA
2371 TGATCA
BglI 2165 GCCGAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2631 GCCGTGCTGGC
BlnI 2578 CCTAGG C^CTAGG
4790 CCTAGG
BlpI 191 GCTGAGC GC^T[ACGT]AGC
3405 GCTAAGC
BpmI 208 CTCCAG CTGGAG(16/14)
792 CTCCAG
Bpu1102I 191 GCTGAGC GC^T[ACGT]AGC
3405 GCTAAGC
BsaWI 521 TCCGGT [AT]^CCGG[AT]
1668 TCCGGT
BsgI 228 CTGCAC GTGCAG(16/14)
730 CTGCAC
BspHI 1100 TCATGA T^CATGA
4745 TCATGA
BssSI 593 CACGAG CACGAG(-5/-1)
3563 CTCGTG
CelII 191 GCTGAGC GC^T[ACGT]AGC
3405 GCTAAGC
EaeI 932 TGGCCG [CT]^GGCC[GA]
2501 TGGCCA
FbaI 1209 TGATCA T^GATCA
2371 TGATCA
HindIII 2853 AAGCTT A^AGCTT
2942 AAGCTT
NcoI 2967 CCATGG C^CATGG
3172 CCATGG
PleI 1215 GACTC GAGTC(4/5)
470 GAGTC
PshBI 1973 ATTAAT AT^TAAT
4665 ATTAAT
PstI 555 CTGCAG CTGCA^G
1469 CTGCAG
RcaI 1100 TCATGA T^CATGA
4745 TCATGA
SexAI 302 ACCTGGT A^CC[AT]GGT
2115 ACCAGGT
SphI 770 GCATGC GCATG^C
1260 GCATGC
SspI 2304 AATATT AAT^ATT
2360 AATATT
XcmI 2159 CCAGGAGCCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3819 CCAGTTATCAGATGG
XmnI 360 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3652 GAAGGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BcgI BcnI
BfaI BmyI BpuAI BsaBI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII