##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccIII AclI AhdI Aor51HI ApaI
AscI AspEI AspI AviII AvrII
BbeI BbvCI BcnI BglI BlnI
BseAI BsiEI Bsp1407I BspDI BspEI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoT14I FseI
FspI HpaI KasI KspI MluI
MroI NarI NciI NcoI NheI
NotI NruI NspV PacI PaeR7I
PflFI PmeI PshAI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspBI
StyI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 732 GACGTC GACGT^C
Acc65I 5361 GGTACC G^GTACC
AccI 5180 GTAGAC GT^[AC][GT]AC
AccII 104 CGCG CG^CG
AflII 5621 CTTAAG C^TTAAG
AgeI 1503 ACCGGT A^CCGGT
Alw44I 4830 GTGCAC G^TGCAC
AlwNI 1892 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1844 CCTGAGG CC^T[ACGT]AGG
ApaLI 4830 GTGCAC G^TGCAC
Asp718I 5361 GGTACC G^GTACC
AvaI 2533 CTCGGG C^[CT]CG[GA]G
BalI 548 TGGCCA TGG^CCA
BamHI 26 GGATCC G^GATCC
BbrPI 2648 CACGTG CAC^GTG
BfrI 5621 CTTAAG C^TTAAG
BsaBI 1407 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1503 ACCGGT [AT]^CCGG[AT]
BsiWI 1186 CGTACG C^GTACG
BsoBI 2533 CTCGGG C^[CT]CG[GA]G
BsrBI 1780 CCGCTC CCGCTC(-3/-3)
BssSI 141 CACGAG CACGAG(-5/-1)
BstAPI 4196 GCATCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1907 GGTCACC G^GT[ACGT]ACC
BstPI 1907 GGTCACC G^GT[ACGT]ACC
BstUI 104 CGCG CG^CG
Bsu36I 1844 CCTGAGG CC^T[ACGT]AGG
DraIII 1558 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1844 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1907 GGTCACC G^GT[ACGT]ACC
EcoRV 507 GATATC GAT^ATC
EcoT22I 2466 ATGCAT ATGCA^T
FauI 4549 GCGGG CCCGC(4/6)
HaeII 700 AGCGCC [GA]GCGC^[CT]
KpnI 5361 GGTACC GGTAC^C
MamI 1407 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 548 TGGCCA TGG^CCA
MscI 548 TGGCCA TGG^CCA
MspA1I 4375 CAGCGG C[AC]G^C[GT]G
MvnI 104 CGCG CG^CG
NaeI 593 GCCGGC GCC^GGC
NdeI 4146 CATATG CA^TATG
NgoMIV 593 GCCGGC G^CCGGC
NsiI 2466 ATGCAT ATGCA^T
PinAI 1503 ACCGGT A^CCGGT
PmaCI 2648 CACGTG CAC^GTG
PmlI 2648 CACGTG CAC^GTG
PpuMI 670 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 627 GAGCTC GAGCT^C
ScaI 1242 AGTACT AGT^ACT
SnaBI 1184 TACGTA TAC^GTA
StuI 3605 AGGCCT AGG^CCT
XbaI 3515 TCTAGA T^CTAGA
XcmI 2741 CCAAGCTACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 732 GACGTC G[GA]^CG[CT]C
4586 GGCGTC
BcgI 1823 GCATTCGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4832 GCACAGGGATCG
BciVI 2396 GTATCC GTATCC(6/5)
5293 GTATCC
BclI 5103 TGATCA T^GATCA
5236 TGATCA
BlpI 422 GCTGAGC GC^T[ACGT]AGC
2091 GCTAAGC
Bpu1102I 422 GCTGAGC GC^T[ACGT]AGC
2091 GCTAAGC
BsaAI 1184 TACGTA [CT]AC^GT[GA]
2648 CACGTG
BsaHI 732 GACGTC G[GA]^CG[CT]C
4586 GGCGTC
BsaI 4470 GAGACC GGTCTC(1/5)
4532 GAGACC
BspMI 2418 ACCTGC ACCTGC(4/8)
4473 ACCTGC
BsrFI 593 GCCGGC [GA]^CCGG[CT]
1503 ACCGGT
BstXI 2826 CCAAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3374 CCAGTGATGTGG
CelII 422 GCTGAGC GC^T[ACGT]AGC
2091 GCTAAGC
Cfr10I 593 GCCGGC [GA]^CCGG[CT]
1503 ACCGGT
DrdI 83 GACTTCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
732 GACGTCAATGTC
DsaI 1957 CCGTGG C^C[GA][CT]GG
2726 CCGTGG
FbaI 5103 TGATCA T^GATCA
5236 TGATCA
HapII 594 CCGG C^CGG
1504 CCGG
Hin1I 732 GACGTC G[GA]^CG[CT]C
4586 GGCGTC
HpaII 594 CCGG C^CGG
1504 CCGG
MfeI 4570 CAATTG C^AATTG
5266 CAATTG
MspI 594 CCGG C^CGG
1504 CCGG
MunI 4570 CAATTG C^AATTG
5266 CAATTG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI Bsp1286I BspHI BspLU11I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoNI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI PstI RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI