################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AccIII AclI AhdI Aor51HI ApaI AscI AspEI AspI AviII AvrII BbeI BbvCI BcnI BglI BlnI BseAI BsiEI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoT14I FseI FspI HpaI KasI KspI MluI MroI NarI NciI NcoI NheI NotI NruI NspV PacI PaeR7I PflFI PmeI PshAI Psp1406I PvuI PvuII RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI StyI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 732 GACGTC GACGT^C Acc65I 5361 GGTACC G^GTACC AccI 5180 GTAGAC GT^[AC][GT]AC AccII 104 CGCG CG^CG AflII 5621 CTTAAG C^TTAAG AgeI 1503 ACCGGT A^CCGGT Alw44I 4830 GTGCAC G^TGCAC AlwNI 1892 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1844 CCTGAGG CC^T[ACGT]AGG ApaLI 4830 GTGCAC G^TGCAC Asp718I 5361 GGTACC G^GTACC AvaI 2533 CTCGGG C^[CT]CG[GA]G BalI 548 TGGCCA TGG^CCA BamHI 26 GGATCC G^GATCC BbrPI 2648 CACGTG CAC^GTG BfrI 5621 CTTAAG C^TTAAG BsaBI 1407 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1503 ACCGGT [AT]^CCGG[AT] BsiWI 1186 CGTACG C^GTACG BsoBI 2533 CTCGGG C^[CT]CG[GA]G BsrBI 1780 CCGCTC CCGCTC(-3/-3) BssSI 141 CACGAG CACGAG(-5/-1) BstAPI 4196 GCATCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1907 GGTCACC G^GT[ACGT]ACC BstPI 1907 GGTCACC G^GT[ACGT]ACC BstUI 104 CGCG CG^CG Bsu36I 1844 CCTGAGG CC^T[ACGT]AGG DraIII 1558 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1844 CCTGAGG CC^T[ACGT]AGG EcoO65I 1907 GGTCACC G^GT[ACGT]ACC EcoRV 507 GATATC GAT^ATC EcoT22I 2466 ATGCAT ATGCA^T FauI 4549 GCGGG CCCGC(4/6) HaeII 700 AGCGCC [GA]GCGC^[CT] KpnI 5361 GGTACC GGTAC^C MamI 1407 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 548 TGGCCA TGG^CCA MscI 548 TGGCCA TGG^CCA MspA1I 4375 CAGCGG C[AC]G^C[GT]G MvnI 104 CGCG CG^CG NaeI 593 GCCGGC GCC^GGC NdeI 4146 CATATG CA^TATG NgoMIV 593 GCCGGC G^CCGGC NsiI 2466 ATGCAT ATGCA^T PinAI 1503 ACCGGT A^CCGGT PmaCI 2648 CACGTG CAC^GTG PmlI 2648 CACGTG CAC^GTG PpuMI 670 AGGTCCC [GA]G^G[AT]CC[CT] SacI 627 GAGCTC GAGCT^C ScaI 1242 AGTACT AGT^ACT SnaBI 1184 TACGTA TAC^GTA StuI 3605 AGGCCT AGG^CCT XbaI 3515 TCTAGA T^CTAGA XcmI 2741 CCAAGCTACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 732 GACGTC G[GA]^CG[CT]C 4586 GGCGTC BcgI 1823 GCATTCGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4832 GCACAGGGATCG BciVI 2396 GTATCC GTATCC(6/5) 5293 GTATCC BclI 5103 TGATCA T^GATCA 5236 TGATCA BlpI 422 GCTGAGC GC^T[ACGT]AGC 2091 GCTAAGC Bpu1102I 422 GCTGAGC GC^T[ACGT]AGC 2091 GCTAAGC BsaAI 1184 TACGTA [CT]AC^GT[GA] 2648 CACGTG BsaHI 732 GACGTC G[GA]^CG[CT]C 4586 GGCGTC BsaI 4470 GAGACC GGTCTC(1/5) 4532 GAGACC BspMI 2418 ACCTGC ACCTGC(4/8) 4473 ACCTGC BsrFI 593 GCCGGC [GA]^CCGG[CT] 1503 ACCGGT BstXI 2826 CCAAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3374 CCAGTGATGTGG CelII 422 GCTGAGC GC^T[ACGT]AGC 2091 GCTAAGC Cfr10I 593 GCCGGC [GA]^CCGG[CT] 1503 ACCGGT DrdI 83 GACTTCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 732 GACGTCAATGTC DsaI 1957 CCGTGG C^C[GA][CT]GG 2726 CCGTGG FbaI 5103 TGATCA T^GATCA 5236 TGATCA HapII 594 CCGG C^CGG 1504 CCGG Hin1I 732 GACGTC G[GA]^CG[CT]C 4586 GGCGTC HpaII 594 CCGG C^CGG 1504 CCGG MfeI 4570 CAATTG C^AATTG 5266 CAATTG MspI 594 CCGG C^CGG 1504 CCGG MunI 4570 CAATTG C^AATTG 5266 CAATTG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BanI BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I BspHI BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoNI EcoO109I EcoRII Fnu4HI FokI HaeIII HgaI HhaI HinP1I HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PstI RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI