##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

AatII       AccIII      AclI        AhdI        ApaI        
AscI        AseI        AsnI        AspEI       BbeI        
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BssHII      ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco52I      EcoRV       FseI        KasI        
KspI        MluI        MroI        NarI        NdeI        
NotI        NruI        PacI        PmeI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SwaI        XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I       3629  GGTACC               G^GTACC
AccII        5521  CGCG                 CG^CG
AcyI         4333  GACGCC               G[GA]^CG[CT]C
AgeI         2324  ACCGGT               A^CCGGT
Aor51HI      3195  AGCGCT               AGC^GCT
Asp718I      3629  GGTACC               G^GTACC
AspI         3703  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1467  TGCGCA               TGC^GCA
AvrII        4598  CCTAGG               C^CTAGG
BcgI         2090  CGATCACTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         5043  TGATCA               T^GATCA
BglII        1899  AGATCT               A^GATCT
BlnI         4598  CCTAGG               C^CTAGG
BsaBI        4428  GATTATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        4333  GACGCC               G[GA]^CG[CT]C
BsaI         1551  GGTCTC               GGTCTC(1/5)
BsmBI         434  CGTCTC               CGTCTC(1/5)
BssSI        3373  CACGAG               CACGAG(-5/-1)
Bst1107I     6352  GTATAC               GTA^TAC
BstEII       1819  GGTGACC              G^GT[ACGT]ACC
BstPI        1819  GGTGACC              G^GT[ACGT]ACC
BstUI        5521  CGCG                 CG^CG
BstZ17I      6352  GTATAC               GTA^TAC
DrdI         3112  GACCTCTGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3195  AGCGCT               AGC^GCT
EcoO65I      1819  GGTGACC              G^GT[ACGT]ACC
FbaI         5043  TGATCA               T^GATCA
FspI         1467  TGCGCA               TGC^GCA
Hin1I        4333  GACGCC               G[GA]^CG[CT]C
HindIII      4270  AAGCTT               A^AGCTT
HpaI          232  GTTAAC               GTT^AAC
KpnI         3629  GGTACC               GGTAC^C
MamI         4428  GATTATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         6181  CAATTG               C^AATTG
MunI         6181  CAATTG               C^AATTG
MvnI         5521  CGCG                 CG^CG
NaeI         4732  GCCGGC               GCC^GGC
NcoI         2210  CCATGG               C^CATGG
NgoMIV       4732  GCCGGC               G^CCGGC
PaeR7I       1718  CTCGAG               C^TCGAG
PflFI        3703  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        2324  ACCGGT               A^CCGGT
PshAI        2386  GACTCTAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI         4465  GAAGAGC              GCTCTTC(1/4)
Tth111I      3703  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1494  CCAATTTAATAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1718  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AflII        2812  CTTAAG               C^TTAAG
             3711  CTTAAG              
AflIII        849  ACATGT               A^C[GA][CT]GT
             4301  ACATGT              
Alw44I       2704  GTGCAC               G^TGCAC
             6079  GTGCAC              
ApaLI        2704  GTGCAC               G^TGCAC
             6079  GTGCAC              
BamHI         505  GGATCC               G^GATCC
             4163  GGATCC              
BbrPI        2158  CACGTG               CAC^GTG
             4324  CACGTG              
BfrI         2812  CTTAAG               C^TTAAG
             3711  CTTAAG              
BglI         3084  GCCAGCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4732  GCCGGCTAGGC         
BlpI           73  GCTGAGC              GC^T[ACGT]AGC
             4717  GCTCAGC             
Bpu1102I       73  GCTGAGC              GC^T[ACGT]AGC
             4717  GCTCAGC             
BsaAI        2158  CACGTG               [CT]AC^GT[GA]
             4324  CACGTG              
BspHI        1788  TCATGA               T^CATGA
             3054  TCATGA              
BspLU11I      849  ACATGT               A^CATGT
             4301  ACATGT              
BspMI        5254  ACCTGC               ACCTGC(4/8)
              279  GCAGGT              
BsrBI        3466  GAGCGG               CCGCTC(-3/-3)
             4532  GAGCGG              
BsrDI         452  GCAATG               GCAATG(2/0)
             2363  GCAATG              
BstBI        4241  TTCGAA               TT^CGAA
             5887  TTCGAA              
CelII          73  GCTGAGC              GC^T[ACGT]AGC
             4717  GCTCAGC             
EcoNI        2281  CCTCCACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4916  CCTGCCCCAGG         
EcoRI         817  GAATTC               G^AATTC
             2165  GAATTC              
EcoT22I      3315  ATGCAT               ATGCA^T
             4564  ATGCAT              
HincII        232  GTTAAC               GT[CT]^[GA]AC
              621  GTCAAC              
HindII        232  GTTAAC               GT[CT]^[GA]AC
              621  GTCAAC              
NheI           78  GCTAGC               G^CTAGC
             3192  GCTAGC              
NsiI         3315  ATGCAT               ATGCA^T
             4564  ATGCAT              
NspV         4241  TTCGAA               TT^CGAA
             5887  TTCGAA              
PflMI         109  CCATTCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4167  CCAGGGACTGG         
PmaCI        2158  CACGTG               CAC^GTG
             4324  CACGTG              
PmlI         2158  CACGTG               CAC^GTG
             4324  CACGTG              
RcaI         1788  TCATGA               T^CATGA
             3054  TCATGA              
SexAI        3704  ACCTGGT              A^CC[AT]GGT
             6000  ACCAGGT             
SfuI         4241  TTCGAA               TT^CGAA
             5887  TTCGAA              
Van91I        109  CCATTCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4167  CCAGGGACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BciVI       
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BsrFI       BsrGI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       SspI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI