################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII AccIII AclI AhdI ApaI AscI AseI AsnI AspEI BbeI BseAI BsiEI BsiWI BspDI BspEI BssHII ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRV FseI KasI KspI MluI MroI NarI NdeI NotI NruI PacI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 3629 GGTACC G^GTACC AccII 5521 CGCG CG^CG AcyI 4333 GACGCC G[GA]^CG[CT]C AgeI 2324 ACCGGT A^CCGGT Aor51HI 3195 AGCGCT AGC^GCT Asp718I 3629 GGTACC G^GTACC AspI 3703 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1467 TGCGCA TGC^GCA AvrII 4598 CCTAGG C^CTAGG BcgI 2090 CGATCACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 5043 TGATCA T^GATCA BglII 1899 AGATCT A^GATCT BlnI 4598 CCTAGG C^CTAGG BsaBI 4428 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 4333 GACGCC G[GA]^CG[CT]C BsaI 1551 GGTCTC GGTCTC(1/5) BsmBI 434 CGTCTC CGTCTC(1/5) BssSI 3373 CACGAG CACGAG(-5/-1) Bst1107I 6352 GTATAC GTA^TAC BstEII 1819 GGTGACC G^GT[ACGT]ACC BstPI 1819 GGTGACC G^GT[ACGT]ACC BstUI 5521 CGCG CG^CG BstZ17I 6352 GTATAC GTA^TAC DrdI 3112 GACCTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3195 AGCGCT AGC^GCT EcoO65I 1819 GGTGACC G^GT[ACGT]ACC FbaI 5043 TGATCA T^GATCA FspI 1467 TGCGCA TGC^GCA Hin1I 4333 GACGCC G[GA]^CG[CT]C HindIII 4270 AAGCTT A^AGCTT HpaI 232 GTTAAC GTT^AAC KpnI 3629 GGTACC GGTAC^C MamI 4428 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 6181 CAATTG C^AATTG MunI 6181 CAATTG C^AATTG MvnI 5521 CGCG CG^CG NaeI 4732 GCCGGC GCC^GGC NcoI 2210 CCATGG C^CATGG NgoMIV 4732 GCCGGC G^CCGGC PaeR7I 1718 CTCGAG C^TCGAG PflFI 3703 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2324 ACCGGT A^CCGGT PshAI 2386 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 4465 GAAGAGC GCTCTTC(1/4) Tth111I 3703 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1494 CCAATTTAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1718 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflII 2812 CTTAAG C^TTAAG 3711 CTTAAG AflIII 849 ACATGT A^C[GA][CT]GT 4301 ACATGT Alw44I 2704 GTGCAC G^TGCAC 6079 GTGCAC ApaLI 2704 GTGCAC G^TGCAC 6079 GTGCAC BamHI 505 GGATCC G^GATCC 4163 GGATCC BbrPI 2158 CACGTG CAC^GTG 4324 CACGTG BfrI 2812 CTTAAG C^TTAAG 3711 CTTAAG BglI 3084 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4732 GCCGGCTAGGC BlpI 73 GCTGAGC GC^T[ACGT]AGC 4717 GCTCAGC Bpu1102I 73 GCTGAGC GC^T[ACGT]AGC 4717 GCTCAGC BsaAI 2158 CACGTG [CT]AC^GT[GA] 4324 CACGTG BspHI 1788 TCATGA T^CATGA 3054 TCATGA BspLU11I 849 ACATGT A^CATGT 4301 ACATGT BspMI 5254 ACCTGC ACCTGC(4/8) 279 GCAGGT BsrBI 3466 GAGCGG CCGCTC(-3/-3) 4532 GAGCGG BsrDI 452 GCAATG GCAATG(2/0) 2363 GCAATG BstBI 4241 TTCGAA TT^CGAA 5887 TTCGAA CelII 73 GCTGAGC GC^T[ACGT]AGC 4717 GCTCAGC EcoNI 2281 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4916 CCTGCCCCAGG EcoRI 817 GAATTC G^AATTC 2165 GAATTC EcoT22I 3315 ATGCAT ATGCA^T 4564 ATGCAT HincII 232 GTTAAC GT[CT]^[GA]AC 621 GTCAAC HindII 232 GTTAAC GT[CT]^[GA]AC 621 GTCAAC NheI 78 GCTAGC G^CTAGC 3192 GCTAGC NsiI 3315 ATGCAT ATGCA^T 4564 ATGCAT NspV 4241 TTCGAA TT^CGAA 5887 TTCGAA PflMI 109 CCATTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4167 CCAGGGACTGG PmaCI 2158 CACGTG CAC^GTG 4324 CACGTG PmlI 2158 CACGTG CAC^GTG 4324 CACGTG RcaI 1788 TCATGA T^CATGA 3054 TCATGA SexAI 3704 ACCTGGT A^CC[AT]GGT 6000 ACCAGGT SfuI 4241 TTCGAA TT^CGAA 5887 TTCGAA Van91I 109 CCATTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4167 CCAGGGACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI