##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccIII AclI AhdI ApaI
AscI AseI AsnI AspEI BbeI
BseAI BsiEI BsiWI BspDI BspEI
BssHII ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoRV FseI KasI
KspI MluI MroI NarI NdeI
NotI NruI PacI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3629 GGTACC G^GTACC
AccII 5521 CGCG CG^CG
AcyI 4333 GACGCC G[GA]^CG[CT]C
AgeI 2324 ACCGGT A^CCGGT
Aor51HI 3195 AGCGCT AGC^GCT
Asp718I 3629 GGTACC G^GTACC
AspI 3703 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1467 TGCGCA TGC^GCA
AvrII 4598 CCTAGG C^CTAGG
BcgI 2090 CGATCACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5043 TGATCA T^GATCA
BglII 1899 AGATCT A^GATCT
BlnI 4598 CCTAGG C^CTAGG
BsaBI 4428 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4333 GACGCC G[GA]^CG[CT]C
BsaI 1551 GGTCTC GGTCTC(1/5)
BsmBI 434 CGTCTC CGTCTC(1/5)
BssSI 3373 CACGAG CACGAG(-5/-1)
Bst1107I 6352 GTATAC GTA^TAC
BstEII 1819 GGTGACC G^GT[ACGT]ACC
BstPI 1819 GGTGACC G^GT[ACGT]ACC
BstUI 5521 CGCG CG^CG
BstZ17I 6352 GTATAC GTA^TAC
DrdI 3112 GACCTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3195 AGCGCT AGC^GCT
EcoO65I 1819 GGTGACC G^GT[ACGT]ACC
FbaI 5043 TGATCA T^GATCA
FspI 1467 TGCGCA TGC^GCA
Hin1I 4333 GACGCC G[GA]^CG[CT]C
HindIII 4270 AAGCTT A^AGCTT
HpaI 232 GTTAAC GTT^AAC
KpnI 3629 GGTACC GGTAC^C
MamI 4428 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6181 CAATTG C^AATTG
MunI 6181 CAATTG C^AATTG
MvnI 5521 CGCG CG^CG
NaeI 4732 GCCGGC GCC^GGC
NcoI 2210 CCATGG C^CATGG
NgoMIV 4732 GCCGGC G^CCGGC
PaeR7I 1718 CTCGAG C^TCGAG
PflFI 3703 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2324 ACCGGT A^CCGGT
PshAI 2386 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 4465 GAAGAGC GCTCTTC(1/4)
Tth111I 3703 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1494 CCAATTTAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1718 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflII 2812 CTTAAG C^TTAAG
3711 CTTAAG
AflIII 849 ACATGT A^C[GA][CT]GT
4301 ACATGT
Alw44I 2704 GTGCAC G^TGCAC
6079 GTGCAC
ApaLI 2704 GTGCAC G^TGCAC
6079 GTGCAC
BamHI 505 GGATCC G^GATCC
4163 GGATCC
BbrPI 2158 CACGTG CAC^GTG
4324 CACGTG
BfrI 2812 CTTAAG C^TTAAG
3711 CTTAAG
BglI 3084 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4732 GCCGGCTAGGC
BlpI 73 GCTGAGC GC^T[ACGT]AGC
4717 GCTCAGC
Bpu1102I 73 GCTGAGC GC^T[ACGT]AGC
4717 GCTCAGC
BsaAI 2158 CACGTG [CT]AC^GT[GA]
4324 CACGTG
BspHI 1788 TCATGA T^CATGA
3054 TCATGA
BspLU11I 849 ACATGT A^CATGT
4301 ACATGT
BspMI 5254 ACCTGC ACCTGC(4/8)
279 GCAGGT
BsrBI 3466 GAGCGG CCGCTC(-3/-3)
4532 GAGCGG
BsrDI 452 GCAATG GCAATG(2/0)
2363 GCAATG
BstBI 4241 TTCGAA TT^CGAA
5887 TTCGAA
CelII 73 GCTGAGC GC^T[ACGT]AGC
4717 GCTCAGC
EcoNI 2281 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4916 CCTGCCCCAGG
EcoRI 817 GAATTC G^AATTC
2165 GAATTC
EcoT22I 3315 ATGCAT ATGCA^T
4564 ATGCAT
HincII 232 GTTAAC GT[CT]^[GA]AC
621 GTCAAC
HindII 232 GTTAAC GT[CT]^[GA]AC
621 GTCAAC
NheI 78 GCTAGC G^CTAGC
3192 GCTAGC
NsiI 3315 ATGCAT ATGCA^T
4564 ATGCAT
NspV 4241 TTCGAA TT^CGAA
5887 TTCGAA
PflMI 109 CCATTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4167 CCAGGGACTGG
PmaCI 2158 CACGTG CAC^GTG
4324 CACGTG
PmlI 2158 CACGTG CAC^GTG
4324 CACGTG
RcaI 1788 TCATGA T^CATGA
3054 TCATGA
SexAI 3704 ACCTGGT A^CC[AT]GGT
6000 ACCAGGT
SfuI 4241 TTCGAA TT^CGAA
5887 TTCGAA
Van91I 109 CCATTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4167 CCAGGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI