##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII AccII AgeI AocI ApaI
AscI Asp700I AviII AvrII BamHI
BbrPI BlnI BsiWI BsrBI BssHII
BstUI Bsu36I CpoI DrdI Eco81I
EcoRV FseI FspI KspI MfeI
MluI MunI MvnI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PvuI RsrII SacII SalI SbfI
SfiI SgrAI SnaBI SpeI Sse8387I
XmnI
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# ONE CUT ENZYMES ( 70 enzymes) #
###################################
Acc65I 996 GGTACC G^GTACC
AccIII 1925 TCCGGA T^CCGGA
AclI 4055 AACGTT AA^CGTT
AcyI 2989 GGCGCC G[GA]^CG[CT]C
AflII 4822 CTTAAG C^TTAAG
AhdI 589 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5989 GTGCAC G^TGCAC
ApaLI 5989 GTGCAC G^TGCAC
Asp718I 996 GGTACC G^GTACC
AspEI 589 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2444 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2989 GGCGCC GGCGC^C
BbvCI 333 GCTGAGG CCTCAGC(-5/-2)
BciVI 2750 GTATCC GTATCC(6/5)
BfrI 4822 CTTAAG C^TTAAG
BglI 698 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2209 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2989 GGCGCC G[GA]^CG[CT]C
BsaWI 1925 TCCGGA [AT]^CCGG[AT]
BseAI 1925 TCCGGA T^CCGGA
BsiEI 5978 CGGCCG CG[GA][CT]^CG
BsmBI 3126 CGTCTC CGTCTC(1/5)
BspEI 1925 TCCGGA T^CCGGA
BspHI 4074 TCATGA T^CATGA
BspMI 3000 GCAGGT ACCTGC(4/8)
BsrFI 5976 GCCGGC [GA]^CCGG[CT]
BssSI 5048 CTCGTG CACGAG(-5/-1)
Bst1107I 3774 GTATAC GTA^TAC
BstBI 2018 TTCGAA TT^CGAA
BstEII 1548 GGTCACC G^GT[ACGT]ACC
BstPI 1548 GGTCACC G^GT[ACGT]ACC
BstXI 1204 CCACCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3774 GTATAC GTA^TAC
Cfr10I 5976 GCCGGC [GA]^CCGG[CT]
EagI 5978 CGGCCG C^GGCCG
Eam1105I 589 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5978 CGGCCG C^GGCCG
Eco52I 5978 CGGCCG C^GGCCG
EcoNI 153 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1548 GGTCACC G^GT[ACGT]ACC
EcoT22I 5626 ATGCAT ATGCA^T
HgaI 2788 GACGC GACGC(5/10)
Hin1I 2989 GGCGCC G[GA]^CG[CT]C
HpaI 3492 GTTAAC GTT^AAC
KasI 2989 GGCGCC G^GCGCC
KpnI 996 GGTACC GGTAC^C
MamI 2209 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1925 TCCGGA T^CCGGA
NaeI 5976 GCCGGC GCC^GGC
NarI 2989 GGCGCC GG^CGCC
NgoMIV 5976 GCCGGC G^CCGGC
NheI 3729 GCTAGC G^CTAGC
NsiI 5626 ATGCAT ATGCA^T
NspV 2018 TTCGAA TT^CGAA
PaeR7I 2600 CTCGAG C^TCGAG
PflFI 2444 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4715 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4055 AACGTT AA^CGTT
PvuII 3755 CAGCTG CAG^CTG
RcaI 4074 TCATGA T^CATGA
ScaI 1099 AGTACT AGT^ACT
SexAI 1727 ACCTGGT A^CC[AT]GGT
SfoI 2989 GGCGCC GGC^GCC
SfuI 2018 TTCGAA TT^CGAA
SmaI 1775 CCCGGG CCC^GGG
SwaI 4963 ATTTAAAT ATTT^AAAT
Tth111I 2444 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2600 CTCGAG C^TCGAG
XmaCI 1775 CCCGGG C^CCGGG
XmaI 1775 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1745 ACATGT A^C[GA][CT]GT
5601 ACATGT
Aor51HI 1706 AGCGCT AGC^GCT
6048 AGCGCT
AseI 5550 ATTAAT AT^TAAT
5638 ATTAAT
AsnI 5550 ATTAAT AT^TAAT
5638 ATTAAT
BcgI 3376 CGACGTTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
985 GCATTCGAGTCG
BclI 1316 TGATCA T^GATCA
1797 TGATCA
BlpI 561 GCTCAGC GC^T[ACGT]AGC
2398 GCTAAGC
Bpu1102I 561 GCTCAGC GC^T[ACGT]AGC
2398 GCTAAGC
BsaAI 3429 TACGTG [CT]AC^GT[GA]
4494 TACGTG
Bsp1407I 5227 TGTACA T^GTACA
5943 TGTACA
BspLU11I 1745 ACATGT A^CATGT
5601 ACATGT
BsrGI 5227 TGTACA T^GTACA
5943 TGTACA
CelII 561 GCTCAGC GC^T[ACGT]AGC
2398 GCTAAGC
DraIII 3238 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5128 CACTCTGTG
Eco47III 1706 AGCGCT AGC^GCT
6048 AGCGCT
EcoRI 2869 GAATTC G^AATTC
5387 GAATTC
FauI 2306 CCCGC CCCGC(4/6)
3357 GCGGG
FbaI 1316 TGATCA T^GATCA
1797 TGATCA
HindIII 1497 AAGCTT A^AGCTT
2124 AAGCTT
PshBI 5550 ATTAAT AT^TAAT
5638 ATTAAT
SacI 2533 GAGCTC GAGCT^C
2629 GAGCTC
SphI 470 GCATGC GCATG^C
1614 GCATGC
SspBI 5227 TGTACA T^GTACA
5943 TGTACA
StuI 1162 AGGCCT AGG^CCT
1946 AGGCCT
XbaI 1960 TCTAGA T^CTAGA
2919 TCTAGA
XcmI 4187 CCAAGGGTTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4804 CCACAGACAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspDI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
ClaI DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII