################################## # NO CUT ENZYMES ( 46 enzymes) # ################################## AatII AccII AgeI AocI ApaI AscI Asp700I AviII AvrII BamHI BbrPI BlnI BsiWI BsrBI BssHII BstUI Bsu36I CpoI DrdI Eco81I EcoRV FseI FspI KspI MfeI MluI MunI MvnI NotI NruI PacI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SbfI SfiI SgrAI SnaBI SpeI Sse8387I XmnI ################################### # ONE CUT ENZYMES ( 70 enzymes) # ################################### Acc65I 996 GGTACC G^GTACC AccIII 1925 TCCGGA T^CCGGA AclI 4055 AACGTT AA^CGTT AcyI 2989 GGCGCC G[GA]^CG[CT]C AflII 4822 CTTAAG C^TTAAG AhdI 589 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 5989 GTGCAC G^TGCAC ApaLI 5989 GTGCAC G^TGCAC Asp718I 996 GGTACC G^GTACC AspEI 589 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2444 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 2989 GGCGCC GGCGC^C BbvCI 333 GCTGAGG CCTCAGC(-5/-2) BciVI 2750 GTATCC GTATCC(6/5) BfrI 4822 CTTAAG C^TTAAG BglI 698 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 2209 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2989 GGCGCC G[GA]^CG[CT]C BsaWI 1925 TCCGGA [AT]^CCGG[AT] BseAI 1925 TCCGGA T^CCGGA BsiEI 5978 CGGCCG CG[GA][CT]^CG BsmBI 3126 CGTCTC CGTCTC(1/5) BspEI 1925 TCCGGA T^CCGGA BspHI 4074 TCATGA T^CATGA BspMI 3000 GCAGGT ACCTGC(4/8) BsrFI 5976 GCCGGC [GA]^CCGG[CT] BssSI 5048 CTCGTG CACGAG(-5/-1) Bst1107I 3774 GTATAC GTA^TAC BstBI 2018 TTCGAA TT^CGAA BstEII 1548 GGTCACC G^GT[ACGT]ACC BstPI 1548 GGTCACC G^GT[ACGT]ACC BstXI 1204 CCACCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3774 GTATAC GTA^TAC Cfr10I 5976 GCCGGC [GA]^CCGG[CT] EagI 5978 CGGCCG C^GGCCG Eam1105I 589 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 5978 CGGCCG C^GGCCG Eco52I 5978 CGGCCG C^GGCCG EcoNI 153 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1548 GGTCACC G^GT[ACGT]ACC EcoT22I 5626 ATGCAT ATGCA^T HgaI 2788 GACGC GACGC(5/10) Hin1I 2989 GGCGCC G[GA]^CG[CT]C HpaI 3492 GTTAAC GTT^AAC KasI 2989 GGCGCC G^GCGCC KpnI 996 GGTACC GGTAC^C MamI 2209 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1925 TCCGGA T^CCGGA NaeI 5976 GCCGGC GCC^GGC NarI 2989 GGCGCC GG^CGCC NgoMIV 5976 GCCGGC G^CCGGC NheI 3729 GCTAGC G^CTAGC NsiI 5626 ATGCAT ATGCA^T NspV 2018 TTCGAA TT^CGAA PaeR7I 2600 CTCGAG C^TCGAG PflFI 2444 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 4715 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 4055 AACGTT AA^CGTT PvuII 3755 CAGCTG CAG^CTG RcaI 4074 TCATGA T^CATGA ScaI 1099 AGTACT AGT^ACT SexAI 1727 ACCTGGT A^CC[AT]GGT SfoI 2989 GGCGCC GGC^GCC SfuI 2018 TTCGAA TT^CGAA SmaI 1775 CCCGGG CCC^GGG SwaI 4963 ATTTAAAT ATTT^AAAT Tth111I 2444 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2600 CTCGAG C^TCGAG XmaCI 1775 CCCGGG C^CCGGG XmaI 1775 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 1745 ACATGT A^C[GA][CT]GT 5601 ACATGT Aor51HI 1706 AGCGCT AGC^GCT 6048 AGCGCT AseI 5550 ATTAAT AT^TAAT 5638 ATTAAT AsnI 5550 ATTAAT AT^TAAT 5638 ATTAAT BcgI 3376 CGACGTTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 985 GCATTCGAGTCG BclI 1316 TGATCA T^GATCA 1797 TGATCA BlpI 561 GCTCAGC GC^T[ACGT]AGC 2398 GCTAAGC Bpu1102I 561 GCTCAGC GC^T[ACGT]AGC 2398 GCTAAGC BsaAI 3429 TACGTG [CT]AC^GT[GA] 4494 TACGTG Bsp1407I 5227 TGTACA T^GTACA 5943 TGTACA BspLU11I 1745 ACATGT A^CATGT 5601 ACATGT BsrGI 5227 TGTACA T^GTACA 5943 TGTACA CelII 561 GCTCAGC GC^T[ACGT]AGC 2398 GCTAAGC DraIII 3238 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG 5128 CACTCTGTG Eco47III 1706 AGCGCT AGC^GCT 6048 AGCGCT EcoRI 2869 GAATTC G^AATTC 5387 GAATTC FauI 2306 CCCGC CCCGC(4/6) 3357 GCGGG FbaI 1316 TGATCA T^GATCA 1797 TGATCA HindIII 1497 AAGCTT A^AGCTT 2124 AAGCTT PshBI 5550 ATTAAT AT^TAAT 5638 ATTAAT SacI 2533 GAGCTC GAGCT^C 2629 GAGCTC SphI 470 GCATGC GCATG^C 1614 GCATGC SspBI 5227 TGTACA T^GTACA 5943 TGTACA StuI 1162 AGGCCT AGG^CCT 1946 AGGCCT XbaI 1960 TCTAGA T^CTAGA 2919 TCTAGA XcmI 4187 CCAAGGGTTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4804 CCACAGACAGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspDI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I ClaI DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII