##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AviII
AvrII BamHI BbeI BbvCI BclI
BcnI BfrI BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HapII
Hin1I HpaII KasI KpnI KspI
MluI MroI MspI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PvuI RcaI RsrII
SacI SacII SalI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
StuI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AclI 2375 AACGTT AA^CGTT
AlwI 28 GGATC GGATC(4/5)
Asp700I 1411 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2785 TGGCCA TGG^CCA
BanII 70 GAGCCC G[GA]GC[CT]^C
BbrPI 175 CACGTG CAC^GTG
BcgI 850 GCAGAGGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4752 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4954 AGATCT A^GATCT
BsaAI 175 CACGTG [CT]AC^GT[GA]
BsmBI 4814 CGTCTC CGTCTC(1/5)
BstYI 4954 AGATCT [GA]^GATC[CT]
DrdI 3461 GACATTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 905 CCATGG C^C[GA][CT]GG
HpaI 4801 GTTAAC GTT^AAC
MflI 4954 AGATCT [GA]^GATC[CT]
MluNI 2785 TGGCCA TGG^CCA
MscI 2785 TGGCCA TGG^CCA
NcoI 905 CCATGG C^CATGG
PmaCI 175 CACGTG CAC^GTG
PmlI 175 CACGTG CAC^GTG
Psp1406I 2375 AACGTT AA^CGTT
SbfI 1169 CCTGCAGG CCTGCA^GG
SexAI 692 ACCAGGT A^CC[AT]GGT
SpeI 4964 ACTAGT A^CTAGT
SphI 491 GCATGC GCATG^C
Sse8387I 1169 CCTGCAGG CCTGCA^GG
XcmI 1383 CCAAGACACTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 4954 AGATCT [GA]^GATC[CT]
XmnI 1411 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AocI 413 CCTCAGG CC^T[ACGT]AGG
3861 CCTGAGG
AseI 2981 ATTAAT AT^TAAT
4900 ATTAAT
AsnI 2981 ATTAAT AT^TAAT
4900 ATTAAT
AspI 1723 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3590 GACATAGTC
AvaI 73 CCCGAG C^[CT]CG[GA]G
4519 CCCGAG
BciVI 2933 GGATAC GTATCC(6/5)
4643 GTATCC
BpmI 3756 CTCCAG CTGGAG(16/14)
976 CTGGAG
BsaBI 1284 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2980 GATTAATATC
BsaI 328 GAGACC GGTCTC(1/5)
3857 GAGACC
BsgI 1581 CTGCAC GTGCAG(16/14)
1624 CTGCAC
BsoBI 73 CCCGAG C^[CT]CG[GA]G
4519 CCCGAG
BspLU11I 665 ACATGT A^CATGT
830 ACATGT
BstXI 2736 CCATTTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4415 CCAGTTCCTTGG
Bsu36I 413 CCTCAGG CC^T[ACGT]AGG
3861 CCTGAGG
CfoI 489 GCGC GCG^C
1038 GCGC
DraI 1475 TTTAAA TTT^AAA
5251 TTTAAA
EaeI 670 TGGCCG [CT]^GGCC[GA]
2785 TGGCCA
Eco81I 413 CCTCAGG CC^T[ACGT]AGG
3861 CCTGAGG
HgaI 1227 GACGC GACGC(5/10)
1499 GCGTC
HhaI 489 GCGC GCG^C
1038 GCGC
HinP1I 489 GCGC G^CGC
1038 GCGC
HincII 782 GTTGAC GT[CT]^[GA]AC
4801 GTTAAC
HindII 782 GTTGAC GT[CT]^[GA]AC
4801 GTTAAC
HindIII 2943 AAGCTT A^AGCTT
3493 AAGCTT
MamI 1284 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2980 GATTAATATC
NdeI 1819 CATATG CA^TATG
2617 CATATG
PflFI 1723 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3590 GACATAGTC
PpuMI 408 GGGTCCC [GA]G^G[AT]CC[CT]
736 GGGACCT
PshBI 2981 ATTAAT AT^TAAT
4900 ATTAAT
PstI 562 CTGCAG CTGCA^G
1170 CTGCAG
SapI 3840 GCTCTTC GCTCTTC(1/4)
4396 GCTCTTC
TaqI 21 TCGA T^CGA
388 TCGA
Tth111I 1723 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3590 GACATAGTC
TthHB8I 21 TCGA T^CGA
388 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwNI ApaLI ApoI AspHI
AvaII BanI BbsI BbvI BfaI
BmyI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MnlI MseI MslI MspA1I
MunI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I