##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AgeI        AhdI        Aor51HI     
ApaI        AscI        Asp718I     AspEI       AviII       
AvrII       BamHI       BbeI        BbvCI       BclI        
BcnI        BfrI        BlnI        BlpI        Bpu1102I    
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BspHI       BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       FauI        
FbaI        FseI        FspI        HaeII       HapII       
Hin1I       HpaII       KasI        KpnI        KspI        
MluI        MroI        MspI        MvnI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PvuI        RcaI        RsrII       
SacI        SacII       SalI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
StuI        SwaI        XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AclI         2375  AACGTT               AA^CGTT
AlwI           28  GGATC                GGATC(4/5)
Asp700I      1411  GAAAAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         2785  TGGCCA               TGG^CCA
BanII          70  GAGCCC               G[GA]GC[CT]^C
BbrPI         175  CACGTG               CAC^GTG
BcgI          850  GCAGAGGAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         4752  GCCAGTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        4954  AGATCT               A^GATCT
BsaAI         175  CACGTG               [CT]AC^GT[GA]
BsmBI        4814  CGTCTC               CGTCTC(1/5)
BstYI        4954  AGATCT               [GA]^GATC[CT]
DrdI         3461  GACATTAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          905  CCATGG               C^C[GA][CT]GG
HpaI         4801  GTTAAC               GTT^AAC
MflI         4954  AGATCT               [GA]^GATC[CT]
MluNI        2785  TGGCCA               TGG^CCA
MscI         2785  TGGCCA               TGG^CCA
NcoI          905  CCATGG               C^CATGG
PmaCI         175  CACGTG               CAC^GTG
PmlI          175  CACGTG               CAC^GTG
Psp1406I     2375  AACGTT               AA^CGTT
SbfI         1169  CCTGCAGG             CCTGCA^GG
SexAI         692  ACCAGGT              A^CC[AT]GGT
SpeI         4964  ACTAGT               A^CTAGT
SphI          491  GCATGC               GCATG^C
Sse8387I     1169  CCTGCAGG             CCTGCA^GG
XcmI         1383  CCAAGACACTATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        4954  AGATCT               [GA]^GATC[CT]
XmnI         1411  GAAAAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AocI          413  CCTCAGG              CC^T[ACGT]AGG
             3861  CCTGAGG             
AseI         2981  ATTAAT               AT^TAAT
             4900  ATTAAT              
AsnI         2981  ATTAAT               AT^TAAT
             4900  ATTAAT              
AspI         1723  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3590  GACATAGTC           
AvaI           73  CCCGAG               C^[CT]CG[GA]G
             4519  CCCGAG              
BciVI        2933  GGATAC               GTATCC(6/5)
             4643  GTATCC              
BpmI         3756  CTCCAG               CTGGAG(16/14)
              976  CTGGAG              
BsaBI        1284  GATGCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2980  GATTAATATC          
BsaI          328  GAGACC               GGTCTC(1/5)
             3857  GAGACC              
BsgI         1581  CTGCAC               GTGCAG(16/14)
             1624  CTGCAC              
BsoBI          73  CCCGAG               C^[CT]CG[GA]G
             4519  CCCGAG              
BspLU11I      665  ACATGT               A^CATGT
              830  ACATGT              
BstXI        2736  CCATTTTAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4415  CCAGTTCCTTGG        
Bsu36I        413  CCTCAGG              CC^T[ACGT]AGG
             3861  CCTGAGG             
CfoI          489  GCGC                 GCG^C
             1038  GCGC                
DraI         1475  TTTAAA               TTT^AAA
             5251  TTTAAA              
EaeI          670  TGGCCG               [CT]^GGCC[GA]
             2785  TGGCCA              
Eco81I        413  CCTCAGG              CC^T[ACGT]AGG
             3861  CCTGAGG             
HgaI         1227  GACGC                GACGC(5/10)
             1499  GCGTC               
HhaI          489  GCGC                 GCG^C
             1038  GCGC                
HinP1I        489  GCGC                 G^CGC
             1038  GCGC                
HincII        782  GTTGAC               GT[CT]^[GA]AC
             4801  GTTAAC              
HindII        782  GTTGAC               GT[CT]^[GA]AC
             4801  GTTAAC              
HindIII      2943  AAGCTT               A^AGCTT
             3493  AAGCTT              
MamI         1284  GATGCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2980  GATTAATATC          
NdeI         1819  CATATG               CA^TATG
             2617  CATATG              
PflFI        1723  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3590  GACATAGTC           
PpuMI         408  GGGTCCC              [GA]G^G[AT]CC[CT]
              736  GGGACCT             
PshBI        2981  ATTAAT               AT^TAAT
             4900  ATTAAT              
PstI          562  CTGCAG               CTGCA^G
             1170  CTGCAG              
SapI         3840  GCTCTTC              GCTCTTC(1/4)
             4396  GCTCTTC             
TaqI           21  TCGA                 T^CGA
              388  TCGA                
Tth111I      1723  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3590  GACATAGTC           
TthHB8I        21  TCGA                 T^CGA
              388  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
Alw44I      AlwNI       ApaLI       ApoI        AspHI       
AvaII       BanI        BbsI        BbvI        BfaI        
BmyI        BpuAI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    Bsp1407I    BspMI       BsrGI       BsrI        
BssKI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoT14I     EcoT22I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MfeI        MnlI        MseI        MslI        MspA1I      
MunI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PflMI       PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspBI       SspI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Van91I