################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AviII AvrII BamHI BbeI BbvCI BclI BcnI BfrI BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII Hin1I HpaII KasI KpnI KspI MluI MroI MspI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PvuI RcaI RsrII SacI SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AclI 2375 AACGTT AA^CGTT AlwI 28 GGATC GGATC(4/5) Asp700I 1411 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 2785 TGGCCA TGG^CCA BanII 70 GAGCCC G[GA]GC[CT]^C BbrPI 175 CACGTG CAC^GTG BcgI 850 GCAGAGGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 4752 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4954 AGATCT A^GATCT BsaAI 175 CACGTG [CT]AC^GT[GA] BsmBI 4814 CGTCTC CGTCTC(1/5) BstYI 4954 AGATCT [GA]^GATC[CT] DrdI 3461 GACATTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 905 CCATGG C^C[GA][CT]GG HpaI 4801 GTTAAC GTT^AAC MflI 4954 AGATCT [GA]^GATC[CT] MluNI 2785 TGGCCA TGG^CCA MscI 2785 TGGCCA TGG^CCA NcoI 905 CCATGG C^CATGG PmaCI 175 CACGTG CAC^GTG PmlI 175 CACGTG CAC^GTG Psp1406I 2375 AACGTT AA^CGTT SbfI 1169 CCTGCAGG CCTGCA^GG SexAI 692 ACCAGGT A^CC[AT]GGT SpeI 4964 ACTAGT A^CTAGT SphI 491 GCATGC GCATG^C Sse8387I 1169 CCTGCAGG CCTGCA^GG XcmI 1383 CCAAGACACTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 4954 AGATCT [GA]^GATC[CT] XmnI 1411 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AocI 413 CCTCAGG CC^T[ACGT]AGG 3861 CCTGAGG AseI 2981 ATTAAT AT^TAAT 4900 ATTAAT AsnI 2981 ATTAAT AT^TAAT 4900 ATTAAT AspI 1723 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3590 GACATAGTC AvaI 73 CCCGAG C^[CT]CG[GA]G 4519 CCCGAG BciVI 2933 GGATAC GTATCC(6/5) 4643 GTATCC BpmI 3756 CTCCAG CTGGAG(16/14) 976 CTGGAG BsaBI 1284 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2980 GATTAATATC BsaI 328 GAGACC GGTCTC(1/5) 3857 GAGACC BsgI 1581 CTGCAC GTGCAG(16/14) 1624 CTGCAC BsoBI 73 CCCGAG C^[CT]CG[GA]G 4519 CCCGAG BspLU11I 665 ACATGT A^CATGT 830 ACATGT BstXI 2736 CCATTTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4415 CCAGTTCCTTGG Bsu36I 413 CCTCAGG CC^T[ACGT]AGG 3861 CCTGAGG CfoI 489 GCGC GCG^C 1038 GCGC DraI 1475 TTTAAA TTT^AAA 5251 TTTAAA EaeI 670 TGGCCG [CT]^GGCC[GA] 2785 TGGCCA Eco81I 413 CCTCAGG CC^T[ACGT]AGG 3861 CCTGAGG HgaI 1227 GACGC GACGC(5/10) 1499 GCGTC HhaI 489 GCGC GCG^C 1038 GCGC HinP1I 489 GCGC G^CGC 1038 GCGC HincII 782 GTTGAC GT[CT]^[GA]AC 4801 GTTAAC HindII 782 GTTGAC GT[CT]^[GA]AC 4801 GTTAAC HindIII 2943 AAGCTT A^AGCTT 3493 AAGCTT MamI 1284 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2980 GATTAATATC NdeI 1819 CATATG CA^TATG 2617 CATATG PflFI 1723 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3590 GACATAGTC PpuMI 408 GGGTCCC [GA]G^G[AT]CC[CT] 736 GGGACCT PshBI 2981 ATTAAT AT^TAAT 4900 ATTAAT PstI 562 CTGCAG CTGCA^G 1170 CTGCAG SapI 3840 GCTCTTC GCTCTTC(1/4) 4396 GCTCTTC TaqI 21 TCGA T^CGA 388 TCGA Tth111I 1723 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3590 GACATAGTC TthHB8I 21 TCGA T^CGA 388 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI Alw44I AlwNI ApaLI ApoI AspHI AvaII BanI BbsI BbvI BfaI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI Cac8I Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MnlI MseI MslI MspA1I MunI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I