################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AhdI ApaI AscI AspEI AviII AvrII BlnI BsaBI BsiWI BspHI Bst1107I BstBI BstZ17I CpoI EagI Eam1105I EclXI Eco52I EcoNI FseI FspI HindIII HpaI MamI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PflMI PmeI PvuI RcaI RsrII SalI SexAI SfiI SfuI SgrAI SnaBI SpeI SwaI Van91I XbaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 2544 GACGTC GACGT^C Acc65I 1114 GGTACC G^GTACC AgeI 417 ACCGGT A^CCGGT Aor51HI 3781 AGCGCT AGC^GCT Asp718I 1114 GGTACC G^GTACC BbeI 76 GGCGCC GGCGC^C BcgI 2016 GCATCCACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 68 GCCCGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsmBI 3563 CGTCTC CGTCTC(1/5) BspDI 3849 ATCGAT AT^CGAT BssHII 170 GCGCGC G^CGCGC BstAPI 3515 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 3849 ATCGAT AT^CGAT DrdI 6478 GACTGTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3781 AGCGCT AGC^GCT EcoT22I 1269 ATGCAT ATGCA^T HincII 2766 GTCAAC GT[CT]^[GA]AC HindII 2766 GTCAAC GT[CT]^[GA]AC KasI 76 GGCGCC G^GCGCC KpnI 1114 GGTACC GGTAC^C KspI 26 CCGCGG CCGC^GG NarI 76 GGCGCC GG^CGCC NsiI 1269 ATGCAT ATGCA^T PaeR7I 5988 CTCGAG C^TCGAG PinAI 417 ACCGGT A^CCGGT PshAI 4370 GACGTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 26 CCGCGG CCGC^GG SbfI 114 CCTGCAGG CCTGCA^GG ScaI 294 AGTACT AGT^ACT SfoI 76 GGCGCC GGC^GCC SmaI 3410 CCCGGG CCC^GGG SphI 4227 GCATGC GCATG^C Sse8387I 114 CCTGCAGG CCTGCA^GG XhoI 5988 CTCGAG C^TCGAG XmaCI 3410 CCCGGG C^CCGGG XmaI 3410 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccIII 3246 TCCGGA T^CCGGA 4029 TCCGGA AclI 4450 AACGTT AA^CGTT 6161 AACGTT AflII 4728 CTTAAG C^TTAAG 5897 CTTAAG Alw44I 1400 GTGCAC G^TGCAC 1657 GTGCAC AlwNI 118 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 3516 CAGAACCTG AocI 1440 CCTGAGG CC^T[ACGT]AGG 4537 CCTAAGG ApaLI 1400 GTGCAC G^TGCAC 1657 GTGCAC AseI 2208 ATTAAT AT^TAAT 5877 ATTAAT AsnI 2208 ATTAAT AT^TAAT 5877 ATTAAT Asp700I 561 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1093 GAAGGCATTC BamHI 2176 GGATCC G^GATCC 5853 GGATCC BbrPI 2568 CACGTG CAC^GTG 3483 CACGTG BbvCI 261 CCTCAGC CCTCAGC(-5/-2) 1940 GCTGAGG BclI 313 TGATCA T^GATCA 4937 TGATCA BfrI 4728 CTTAAG C^TTAAG 5897 CTTAAG BglII 632 AGATCT A^GATCT 2749 AGATCT BseAI 3246 TCCGGA T^CCGGA 4029 TCCGGA BsiEI 415 CGACCG CG[GA][CT]^CG 419 CGGTCG BspEI 3246 TCCGGA T^CCGGA 4029 TCCGGA BssSI 2285 CACGAG CACGAG(-5/-1) 4800 CACGAG BstEII 605 GGTGACC G^GT[ACGT]ACC 5587 GGTGACC BstPI 605 GGTGACC G^GT[ACGT]ACC 5587 GGTGACC BstXI 3301 CCATCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6604 CCAGATAGCTGG Bsu36I 1440 CCTGAGG CC^T[ACGT]AGG 4537 CCTAAGG DraII 94 GGGTCCC [GA]G^G[ACGT]CC[CT] 1365 GGGACCC DraIII 6386 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 6944 CACTGTGTG Eco81I 1440 CCTGAGG CC^T[ACGT]AGG 4537 CCTAAGG EcoO109I 94 GGGTCCC [GA]G^G[ACGT]CC[CT] 1365 GGGACCC EcoO65I 605 GGTGACC G^GT[ACGT]ACC 5587 GGTGACC EcoRI 965 GAATTC G^AATTC 4958 GAATTC EcoRV 744 GATATC GAT^ATC 6212 GATATC FbaI 313 TGATCA T^GATCA 4937 TGATCA MroI 3246 TCCGGA T^CCGGA 4029 TCCGGA PmaCI 2568 CACGTG CAC^GTG 3483 CACGTG PmlI 2568 CACGTG CAC^GTG 3483 CACGTG PpuMI 94 GGGTCCC [GA]G^G[AT]CC[CT] 1365 GGGACCC PshBI 2208 ATTAAT AT^TAAT 5877 ATTAAT Psp1406I 4450 AACGTT AA^CGTT 6161 AACGTT StuI 3632 AGGCCT AGG^CCT 6345 AGGCCT XmnI 561 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1093 GAAGGCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII