##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AhdI ApaI AscI AspEI AviII
AvrII BlnI BsaBI BsiWI BspHI
Bst1107I BstBI BstZ17I CpoI EagI
Eam1105I EclXI Eco52I EcoNI FseI
FspI HindIII HpaI MamI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflMI PmeI PvuI RcaI RsrII
SalI SexAI SfiI SfuI SgrAI
SnaBI SpeI SwaI Van91I XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2544 GACGTC GACGT^C
Acc65I 1114 GGTACC G^GTACC
AgeI 417 ACCGGT A^CCGGT
Aor51HI 3781 AGCGCT AGC^GCT
Asp718I 1114 GGTACC G^GTACC
BbeI 76 GGCGCC GGCGC^C
BcgI 2016 GCATCCACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 68 GCCCGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 3563 CGTCTC CGTCTC(1/5)
BspDI 3849 ATCGAT AT^CGAT
BssHII 170 GCGCGC G^CGCGC
BstAPI 3515 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 3849 ATCGAT AT^CGAT
DrdI 6478 GACTGTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3781 AGCGCT AGC^GCT
EcoT22I 1269 ATGCAT ATGCA^T
HincII 2766 GTCAAC GT[CT]^[GA]AC
HindII 2766 GTCAAC GT[CT]^[GA]AC
KasI 76 GGCGCC G^GCGCC
KpnI 1114 GGTACC GGTAC^C
KspI 26 CCGCGG CCGC^GG
NarI 76 GGCGCC GG^CGCC
NsiI 1269 ATGCAT ATGCA^T
PaeR7I 5988 CTCGAG C^TCGAG
PinAI 417 ACCGGT A^CCGGT
PshAI 4370 GACGTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 26 CCGCGG CCGC^GG
SbfI 114 CCTGCAGG CCTGCA^GG
ScaI 294 AGTACT AGT^ACT
SfoI 76 GGCGCC GGC^GCC
SmaI 3410 CCCGGG CCC^GGG
SphI 4227 GCATGC GCATG^C
Sse8387I 114 CCTGCAGG CCTGCA^GG
XhoI 5988 CTCGAG C^TCGAG
XmaCI 3410 CCCGGG C^CCGGG
XmaI 3410 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 3246 TCCGGA T^CCGGA
4029 TCCGGA
AclI 4450 AACGTT AA^CGTT
6161 AACGTT
AflII 4728 CTTAAG C^TTAAG
5897 CTTAAG
Alw44I 1400 GTGCAC G^TGCAC
1657 GTGCAC
AlwNI 118 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3516 CAGAACCTG
AocI 1440 CCTGAGG CC^T[ACGT]AGG
4537 CCTAAGG
ApaLI 1400 GTGCAC G^TGCAC
1657 GTGCAC
AseI 2208 ATTAAT AT^TAAT
5877 ATTAAT
AsnI 2208 ATTAAT AT^TAAT
5877 ATTAAT
Asp700I 561 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1093 GAAGGCATTC
BamHI 2176 GGATCC G^GATCC
5853 GGATCC
BbrPI 2568 CACGTG CAC^GTG
3483 CACGTG
BbvCI 261 CCTCAGC CCTCAGC(-5/-2)
1940 GCTGAGG
BclI 313 TGATCA T^GATCA
4937 TGATCA
BfrI 4728 CTTAAG C^TTAAG
5897 CTTAAG
BglII 632 AGATCT A^GATCT
2749 AGATCT
BseAI 3246 TCCGGA T^CCGGA
4029 TCCGGA
BsiEI 415 CGACCG CG[GA][CT]^CG
419 CGGTCG
BspEI 3246 TCCGGA T^CCGGA
4029 TCCGGA
BssSI 2285 CACGAG CACGAG(-5/-1)
4800 CACGAG
BstEII 605 GGTGACC G^GT[ACGT]ACC
5587 GGTGACC
BstPI 605 GGTGACC G^GT[ACGT]ACC
5587 GGTGACC
BstXI 3301 CCATCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6604 CCAGATAGCTGG
Bsu36I 1440 CCTGAGG CC^T[ACGT]AGG
4537 CCTAAGG
DraII 94 GGGTCCC [GA]G^G[ACGT]CC[CT]
1365 GGGACCC
DraIII 6386 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
6944 CACTGTGTG
Eco81I 1440 CCTGAGG CC^T[ACGT]AGG
4537 CCTAAGG
EcoO109I 94 GGGTCCC [GA]G^G[ACGT]CC[CT]
1365 GGGACCC
EcoO65I 605 GGTGACC G^GT[ACGT]ACC
5587 GGTGACC
EcoRI 965 GAATTC G^AATTC
4958 GAATTC
EcoRV 744 GATATC GAT^ATC
6212 GATATC
FbaI 313 TGATCA T^GATCA
4937 TGATCA
MroI 3246 TCCGGA T^CCGGA
4029 TCCGGA
PmaCI 2568 CACGTG CAC^GTG
3483 CACGTG
PmlI 2568 CACGTG CAC^GTG
3483 CACGTG
PpuMI 94 GGGTCCC [GA]G^G[AT]CC[CT]
1365 GGGACCC
PshBI 2208 ATTAAT AT^TAAT
5877 ATTAAT
Psp1406I 4450 AACGTT AA^CGTT
6161 AACGTT
StuI 3632 AGGCCT AGG^CCT
6345 AGGCCT
XmnI 561 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1093 GAAGGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PleI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII