##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccIII AclI AgeI AhdI AscI
AspEI AviII AvrII BbvCI BlnI
BseAI BsiEI BsiWI BsmBI BspEI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FseI
FspI HindIII HpaI KspI MfeI
MluI MroI MunI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SacII
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 2041 GACGTC GACGT^C
AflII 256 CTTAAG C^TTAAG
Alw44I 4877 GTGCAC G^TGCAC
Aor51HI 2543 AGCGCT AGC^GCT
ApaI 13 GGGCCC GGGCC^C
ApaLI 4877 GTGCAC G^TGCAC
Asp700I 6251 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 3584 GGATCC G^GATCC
BbeI 1975 GGCGCC GGCGC^C
BfrI 256 CTTAAG C^TTAAG
BglI 4731 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 36 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 2293 ATCGAT AT^CGAT
BssSI 2566 CTCGTG CACGAG(-5/-1)
ClaI 2293 ATCGAT AT^CGAT
Eco47III 2543 AGCGCT AGC^GCT
EcoRI 5119 GAATTC G^AATTC
KasI 1975 GGCGCC G^GCGCC
MamI 36 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1975 GGCGCC GG^CGCC
NdeI 3092 CATATG CA^TATG
SacI 3463 GAGCTC GAGCT^C
SalI 2659 GTCGAC G^TCGAC
SexAI 3174 ACCAGGT A^CC[AT]GGT
SfoI 1975 GGCGCC GGC^GCC
SmaI 3197 CCCGGG CCC^GGG
StuI 2269 AGGCCT AGG^CCT
XmaCI 3197 CCCGGG C^CCGGG
XmaI 3197 CCCGGG C^CCGGG
XmnI 6251 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2659 GTCGAC GT^[AC][GT]AC
3547 GTAGAC
AseI 5085 ATTAAT AT^TAAT
5787 ATTAAT
AsnI 5085 ATTAAT AT^TAAT
5787 ATTAAT
AspI 1422 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3049 GACCTTGTC
BalI 2001 TGGCCA TGG^CCA
4396 TGGCCA
BbrPI 559 CACGTG CAC^GTG
2490 CACGTG
BcgI 635 GCACCATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1711 GCAGAGGGGTCG
BciVI 3271 GGATAC GTATCC(6/5)
3454 GTATCC
BglII 201 AGATCT A^GATCT
4017 AGATCT
BsaAI 559 CACGTG [CT]AC^GT[GA]
2490 CACGTG
BsaWI 30 ACCGGA [AT]^CCGG[AT]
3569 TCCGGT
BspLU11I 2582 ACATGT A^CATGT
5948 ACATGT
DraIII 652 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
2145 CACAACGTG
EaeI 2001 TGGCCA [CT]^GGCC[GA]
4396 TGGCCA
EcoNI 1431 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3693 CCTTGATGAGG
HaeII 1975 GGCGCC [GA]GCGC^[CT]
2543 AGCGCT
MluNI 2001 TGGCCA TGG^CCA
4396 TGGCCA
MscI 2001 TGGCCA TGG^CCA
4396 TGGCCA
NaeI 440 GCCGGC GCC^GGC
4000 GCCGGC
NgoMIV 440 GCCGGC G^CCGGC
4000 GCCGGC
PflFI 1422 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3049 GACCTTGTC
PmaCI 559 CACGTG CAC^GTG
2490 CACGTG
PmlI 559 CACGTG CAC^GTG
2490 CACGTG
PshAI 396 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2338 GACTTCCGTC
PshBI 5085 ATTAAT AT^TAAT
5787 ATTAAT
Tth111I 1422 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3049 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI Asp718I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BclI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EarI Eco81I EcoO109I
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII