################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AccIII AclI AgeI AhdI AscI AspEI AviII AvrII BbvCI BlnI BseAI BsiEI BsiWI BsmBI BspEI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV FseI FspI HindIII HpaI KspI MfeI MluI MroI MunI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacII SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AatII 2041 GACGTC GACGT^C AflII 256 CTTAAG C^TTAAG Alw44I 4877 GTGCAC G^TGCAC Aor51HI 2543 AGCGCT AGC^GCT ApaI 13 GGGCCC GGGCC^C ApaLI 4877 GTGCAC G^TGCAC Asp700I 6251 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 3584 GGATCC G^GATCC BbeI 1975 GGCGCC GGCGC^C BfrI 256 CTTAAG C^TTAAG BglI 4731 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 36 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspDI 2293 ATCGAT AT^CGAT BssSI 2566 CTCGTG CACGAG(-5/-1) ClaI 2293 ATCGAT AT^CGAT Eco47III 2543 AGCGCT AGC^GCT EcoRI 5119 GAATTC G^AATTC KasI 1975 GGCGCC G^GCGCC MamI 36 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1975 GGCGCC GG^CGCC NdeI 3092 CATATG CA^TATG SacI 3463 GAGCTC GAGCT^C SalI 2659 GTCGAC G^TCGAC SexAI 3174 ACCAGGT A^CC[AT]GGT SfoI 1975 GGCGCC GGC^GCC SmaI 3197 CCCGGG CCC^GGG StuI 2269 AGGCCT AGG^CCT XmaCI 3197 CCCGGG C^CCGGG XmaI 3197 CCCGGG C^CCGGG XmnI 6251 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 2659 GTCGAC GT^[AC][GT]AC 3547 GTAGAC AseI 5085 ATTAAT AT^TAAT 5787 ATTAAT AsnI 5085 ATTAAT AT^TAAT 5787 ATTAAT AspI 1422 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3049 GACCTTGTC BalI 2001 TGGCCA TGG^CCA 4396 TGGCCA BbrPI 559 CACGTG CAC^GTG 2490 CACGTG BcgI 635 GCACCATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1711 GCAGAGGGGTCG BciVI 3271 GGATAC GTATCC(6/5) 3454 GTATCC BglII 201 AGATCT A^GATCT 4017 AGATCT BsaAI 559 CACGTG [CT]AC^GT[GA] 2490 CACGTG BsaWI 30 ACCGGA [AT]^CCGG[AT] 3569 TCCGGT BspLU11I 2582 ACATGT A^CATGT 5948 ACATGT DraIII 652 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG 2145 CACAACGTG EaeI 2001 TGGCCA [CT]^GGCC[GA] 4396 TGGCCA EcoNI 1431 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3693 CCTTGATGAGG HaeII 1975 GGCGCC [GA]GCGC^[CT] 2543 AGCGCT MluNI 2001 TGGCCA TGG^CCA 4396 TGGCCA MscI 2001 TGGCCA TGG^CCA 4396 TGGCCA NaeI 440 GCCGGC GCC^GGC 4000 GCCGGC NgoMIV 440 GCCGGC G^CCGGC 4000 GCCGGC PflFI 1422 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3049 GACCTTGTC PmaCI 559 CACGTG CAC^GTG 2490 CACGTG PmlI 559 CACGTG CAC^GTG 2490 CACGTG PshAI 396 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2338 GACTTCCGTC PshBI 5085 ATTAAT AT^TAAT 5787 ATTAAT Tth111I 1422 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3049 GACCTTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### Acc65I AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp718I AspHI AvaI AvaII BanI BanII BbsI BbvI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII