##################################
# NO CUT ENZYMES (   50 enzymes) #
##################################

AccIII      AclI        AgeI        AscI        AseI        
AsnI        BamHI       BbrPI       BcgI        BciVI       
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        EagI        EclXI       Eco52I      EcoRV       
FseI        HgaI        HindIII     MluI        MroI        
NdeI        NotI        NruI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SalI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SwaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII        3056  GACGTC               GACGT^C
Acc65I       4324  GGTACC               G^GTACC
AflII         282  CTTAAG               C^TTAAG
AhdI         1402  GACCTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        737  GTGCAC               G^TGCAC
Aor51HI       669  AGCGCT               AGC^GCT
ApaLI         737  GTGCAC               G^TGCAC
Asp718I      4324  GGTACC               G^GTACC
AspEI        1402  GACCTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         4099  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        3508  TGCGCA               TGC^GCA
BbvCI        4186  GCTGAGG              CCTCAGC(-5/-2)
BclI         3856  TGATCA               T^GATCA
BfrI          282  CTTAAG               C^TTAAG
BglI          315  GCCGTGACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1942  AGATCT               A^GATCT
BlpI         4117  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     4117  GCTCAGC              GC^T[ACGT]AGC
BsaAI        4879  CACGTA               [CT]AC^GT[GA]
BsaBI        2406  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        3058  CGTCTC               CGTCTC(1/5)
Bsp1407I     2154  TGTACA               T^GTACA
BsrBI        4238  GAGCGG               CCGCTC(-3/-3)
BsrGI        2154  TGTACA               T^GTACA
BssSI        4136  CTCGTG               CACGAG(-5/-1)
CelII        4117  GCTCAGC              GC^T[ACGT]AGC
DraIII       1812  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1402  GACCTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      669  AGCGCT               AGC^GCT
EcoNI        3464  CCTGGGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      2880  ATGCAT               ATGCA^T
FbaI         3856  TGATCA               T^GATCA
FspI         3508  TGCGCA               TGC^GCA
HpaI         1301  GTTAAC               GTT^AAC
KpnI         4324  GGTACC               GGTAC^C
MamI         2406  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         5394  CAATTG               C^AATTG
MunI         5394  CAATTG               C^AATTG
NsiI         2880  ATGCAT               ATGCA^T
PaeR7I       4806  CTCGAG               C^TCGAG
PflFI        4099  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacI         3477  GAGCTC               GAGCT^C
SbfI          623  CCTGCAGG             CCTGCA^GG
SexAI        2293  ACCAGGT              A^CC[AT]GGT
Sse8387I      623  CCTGCAGG             CCTGCA^GG
SspBI        2154  TGTACA               T^GTACA
Tth111I      4099  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         4806  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         1383  GTCTAC               GT^[AC][GT]AC
             1796  GTCTAC              
AvrII        3962  CCTAGG               C^CTAGG
             4906  CCTAGG              
BbeI           84  GGCGCC               GGCGC^C
             4245  GGCGCC              
BbsI         3611  GAAGAC               GAAGAC(2/6)
             4277  GAAGAC              
BlnI         3962  CCTAGG               C^CTAGG
             4906  CCTAGG              
BpuAI        3611  GAAGAC               GAAGAC(2/6)
             4277  GAAGAC              
BsaWI        3872  ACCGGA               [AT]^CCGG[AT]
             4596  TCCGGT              
BsmI         3197  GAATGC               GAATGC(1/-1)
             4937  GCATTC              
BspHI        1903  TCATGA               T^CATGA
             2920  TCATGA              
BspLU11I     5143  ACATGT               A^CATGT
             6054  ACATGT              
DrdI         1787  GACCACAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6042  GACATCGTGGTC        
EcoRI         748  GAATTC               G^AATTC
             5266  GAATTC              
KasI           84  GGCGCC               G^GCGCC
             4245  GGCGCC              
KspI           36  CCGCGG               CCGC^GG
              174  CCGCGG              
NaeI           16  GCCGGC               GCC^GGC
             4024  GCCGGC              
NarI           84  GGCGCC               GG^CGCC
             4245  GGCGCC              
NgoMIV         16  GCCGGC               G^CCGGC
             4024  GCCGGC              
PshAI        2532  GACCCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4947  GACAGCTGTC          
RcaI         1903  TCATGA               T^CATGA
             2920  TCATGA              
SacII          36  CCGCGG               CCGC^GG
              174  CCGCGG              
SapI         3474  GAAGAGC              GCTCTTC(1/4)
             1498  GCTCTTC             
ScaI         1180  AGTACT               AGT^ACT
             1194  AGTACT              
SfoI           84  GGCGCC               GGC^GCC
             4245  GGCGCC              
SmaI         3282  CCCGGG               CCC^GGG
             5887  CCCGGG              
SphI         2622  GCATGC               GCATG^C
             3971  GCATGC              
SspI         2968  AATATT               AAT^ATT
             4408  AATATT              
XbaI         1929  TCTAGA               T^CTAGA
             1939  TCTAGA              
XmaCI        3282  CCCGGG               C^CCGGG
             5887  CCCGGG              
XmaI         3282  CCCGGG               C^CCGGG
             5887  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApaI        ApoI        Asp700I     AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoO65I     EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NheI        NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmnI