##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AccIII AclI AgeI AscI AseI
AsnI BamHI BbrPI BcgI BciVI
BseAI BsiEI BsiWI BspDI BspEI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI EclXI Eco52I EcoRV
FseI HgaI HindIII MluI MroI
NdeI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SfiI
SfuI SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 3056 GACGTC GACGT^C
Acc65I 4324 GGTACC G^GTACC
AflII 282 CTTAAG C^TTAAG
AhdI 1402 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 737 GTGCAC G^TGCAC
Aor51HI 669 AGCGCT AGC^GCT
ApaLI 737 GTGCAC G^TGCAC
Asp718I 4324 GGTACC G^GTACC
AspEI 1402 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4099 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3508 TGCGCA TGC^GCA
BbvCI 4186 GCTGAGG CCTCAGC(-5/-2)
BclI 3856 TGATCA T^GATCA
BfrI 282 CTTAAG C^TTAAG
BglI 315 GCCGTGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1942 AGATCT A^GATCT
BlpI 4117 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4117 GCTCAGC GC^T[ACGT]AGC
BsaAI 4879 CACGTA [CT]AC^GT[GA]
BsaBI 2406 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3058 CGTCTC CGTCTC(1/5)
Bsp1407I 2154 TGTACA T^GTACA
BsrBI 4238 GAGCGG CCGCTC(-3/-3)
BsrGI 2154 TGTACA T^GTACA
BssSI 4136 CTCGTG CACGAG(-5/-1)
CelII 4117 GCTCAGC GC^T[ACGT]AGC
DraIII 1812 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1402 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 669 AGCGCT AGC^GCT
EcoNI 3464 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2880 ATGCAT ATGCA^T
FbaI 3856 TGATCA T^GATCA
FspI 3508 TGCGCA TGC^GCA
HpaI 1301 GTTAAC GTT^AAC
KpnI 4324 GGTACC GGTAC^C
MamI 2406 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5394 CAATTG C^AATTG
MunI 5394 CAATTG C^AATTG
NsiI 2880 ATGCAT ATGCA^T
PaeR7I 4806 CTCGAG C^TCGAG
PflFI 4099 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 3477 GAGCTC GAGCT^C
SbfI 623 CCTGCAGG CCTGCA^GG
SexAI 2293 ACCAGGT A^CC[AT]GGT
Sse8387I 623 CCTGCAGG CCTGCA^GG
SspBI 2154 TGTACA T^GTACA
Tth111I 4099 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4806 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1383 GTCTAC GT^[AC][GT]AC
1796 GTCTAC
AvrII 3962 CCTAGG C^CTAGG
4906 CCTAGG
BbeI 84 GGCGCC GGCGC^C
4245 GGCGCC
BbsI 3611 GAAGAC GAAGAC(2/6)
4277 GAAGAC
BlnI 3962 CCTAGG C^CTAGG
4906 CCTAGG
BpuAI 3611 GAAGAC GAAGAC(2/6)
4277 GAAGAC
BsaWI 3872 ACCGGA [AT]^CCGG[AT]
4596 TCCGGT
BsmI 3197 GAATGC GAATGC(1/-1)
4937 GCATTC
BspHI 1903 TCATGA T^CATGA
2920 TCATGA
BspLU11I 5143 ACATGT A^CATGT
6054 ACATGT
DrdI 1787 GACCACAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6042 GACATCGTGGTC
EcoRI 748 GAATTC G^AATTC
5266 GAATTC
KasI 84 GGCGCC G^GCGCC
4245 GGCGCC
KspI 36 CCGCGG CCGC^GG
174 CCGCGG
NaeI 16 GCCGGC GCC^GGC
4024 GCCGGC
NarI 84 GGCGCC GG^CGCC
4245 GGCGCC
NgoMIV 16 GCCGGC G^CCGGC
4024 GCCGGC
PshAI 2532 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4947 GACAGCTGTC
RcaI 1903 TCATGA T^CATGA
2920 TCATGA
SacII 36 CCGCGG CCGC^GG
174 CCGCGG
SapI 3474 GAAGAGC GCTCTTC(1/4)
1498 GCTCTTC
ScaI 1180 AGTACT AGT^ACT
1194 AGTACT
SfoI 84 GGCGCC GGC^GCC
4245 GGCGCC
SmaI 3282 CCCGGG CCC^GGG
5887 CCCGGG
SphI 2622 GCATGC GCATG^C
3971 GCATGC
SspI 2968 AATATT AAT^ATT
4408 AATATT
XbaI 1929 TCTAGA T^CTAGA
1939 TCTAGA
XmaCI 3282 CCCGGG C^CCGGG
5887 CCCGGG
XmaI 3282 CCCGGG C^CCGGG
5887 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NheI NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI