################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII AcyI AgeI AhdI AscI AspEI AviII AvrII BbeI BclI BlnI BsaBI BsaHI BsiEI BssHII BstAPI BstBI CpoI EagI Eam1105I EclXI Eco52I FbaI FseI FspI Hin1I HindIII KasI KspI MamI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmeI PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccIII 1299 TCCGGA T^CCGGA AclI 3623 AACGTT AA^CGTT Alw44I 374 GTGCAC G^TGCAC ApaLI 374 GTGCAC G^TGCAC AseI 5031 ATTAAT AT^TAAT AsnI 5031 ATTAAT AT^TAAT AspI 655 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1302 GGATCC G^GATCC BglII 1912 AGATCT A^GATCT BlpI 4017 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4017 GCTGAGC GC^T[ACGT]AGC BseAI 1299 TCCGGA T^CCGGA BsiWI 457 CGTACG C^GTACG BspDI 2752 ATCGAT AT^CGAT BspEI 1299 TCCGGA T^CCGGA Bst1107I 3611 GTATAC GTA^TAC BstZ17I 3611 GTATAC GTA^TAC CelII 4017 GCTGAGC GC^T[ACGT]AGC ClaI 2752 ATCGAT AT^CGAT EcoRV 71 GATATC GAT^ATC EcoT22I 4994 ATGCAT ATGCA^T HpaI 2696 GTTAAC GTT^AAC MfeI 5345 CAATTG C^AATTG MroI 1299 TCCGGA T^CCGGA MunI 5345 CAATTG C^AATTG NsiI 4994 ATGCAT ATGCA^T PflFI 655 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 5031 ATTAAT AT^TAAT Psp1406I 3623 AACGTT AA^CGTT Tth111I 655 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3110 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 2848 GGTACC G^GTACC 3868 GGTACC AflII 3156 CTTAAG C^TTAAG 3949 CTTAAG Aor51HI 438 AGCGCT AGC^GCT 4703 AGCGCT ApaI 1245 GGGCCC GGGCC^C 3724 GGGCCC Asp718I 2848 GGTACC G^GTACC 3868 GGTACC BbvCI 3791 CCTCAGC CCTCAGC(-5/-2) 3814 CCTCAGC BcgI 627 CGACAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3381 CGAGCCAGGTGC BfrI 3156 CTTAAG C^TTAAG 3949 CTTAAG BglI 399 GCCACGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 926 GCCCTCTGGGC BsaWI 1299 TCCGGA [AT]^CCGG[AT] 2049 ACCGGA BsmBI 1665 CGTCTC CGTCTC(1/5) 2393 GAGACG BsmI 1882 GCATTC GAATGC(1/-1) 2818 GCATTC Bsp1407I 4299 TGTACA T^GTACA 4446 TGTACA BspHI 3402 TCATGA T^CATGA 5267 TCATGA BspLU11I 2604 ACATGT A^CATGT 4253 ACATGT BsrDI 1161 GCAATG GCAATG(2/0) 5512 GCAATG BsrFI 1115 GCCGGT [GA]^CCGG[CT] 2851 ACCGGC BsrGI 4299 TGTACA T^GTACA 4446 TGTACA BstEII 2199 GGTCACC G^GT[ACGT]ACC 3765 GGTCACC BstPI 2199 GGTCACC G^GT[ACGT]ACC 3765 GGTCACC Cfr10I 1115 GCCGGT [GA]^CCGG[CT] 2851 ACCGGC DraI 3000 TTTAAA TTT^AAA 4892 TTTAAA DraIII 959 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG 5316 CACTGTGTG DrdI 1657 GACCGACGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1771 GACAGCCTGGTC Eco47III 438 AGCGCT AGC^GCT 4703 AGCGCT EcoO65I 2199 GGTCACC G^GT[ACGT]ACC 3765 GGTCACC EcoRI 2123 GAATTC G^AATTC 3114 GAATTC KpnI 2848 GGTACC GGTAC^C 3868 GGTACC MluI 1662 ACGCGT A^CGCGT 2396 ACGCGT NcoI 2424 CCATGG C^CATGG 3437 CCATGG NheI 11 GCTAGC G^CTAGC 4271 GCTAGC PshAI 1732 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5100 GACCTCTGTC RcaI 3402 TCATGA T^CATGA 5267 TCATGA ScaI 2090 AGTACT AGT^ACT 3844 AGTACT SexAI 1023 ACCAGGT A^CC[AT]GGT 4325 ACCAGGT SmlI 3156 CTTAAG C^T[CT][GA]AG 3949 CTTAAG SspBI 4299 TGTACA T^GTACA 4446 TGTACA SspI 1529 AATATT AAT^ATT 5504 AATATT StuI 265 AGGCCT AGG^CCT 4763 AGGCCT XcmI 93 CCAACGATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4467 CCACGTGGGTACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI XmnI