##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AcyI AgeI AhdI AscI
AspEI AviII AvrII BbeI BclI
BlnI BsaBI BsaHI BsiEI BssHII
BstAPI BstBI CpoI EagI Eam1105I
EclXI Eco52I FbaI FseI FspI
Hin1I HindIII KasI KspI MamI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmeI PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 1299 TCCGGA T^CCGGA
AclI 3623 AACGTT AA^CGTT
Alw44I 374 GTGCAC G^TGCAC
ApaLI 374 GTGCAC G^TGCAC
AseI 5031 ATTAAT AT^TAAT
AsnI 5031 ATTAAT AT^TAAT
AspI 655 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1302 GGATCC G^GATCC
BglII 1912 AGATCT A^GATCT
BlpI 4017 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4017 GCTGAGC GC^T[ACGT]AGC
BseAI 1299 TCCGGA T^CCGGA
BsiWI 457 CGTACG C^GTACG
BspDI 2752 ATCGAT AT^CGAT
BspEI 1299 TCCGGA T^CCGGA
Bst1107I 3611 GTATAC GTA^TAC
BstZ17I 3611 GTATAC GTA^TAC
CelII 4017 GCTGAGC GC^T[ACGT]AGC
ClaI 2752 ATCGAT AT^CGAT
EcoRV 71 GATATC GAT^ATC
EcoT22I 4994 ATGCAT ATGCA^T
HpaI 2696 GTTAAC GTT^AAC
MfeI 5345 CAATTG C^AATTG
MroI 1299 TCCGGA T^CCGGA
MunI 5345 CAATTG C^AATTG
NsiI 4994 ATGCAT ATGCA^T
PflFI 655 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 5031 ATTAAT AT^TAAT
Psp1406I 3623 AACGTT AA^CGTT
Tth111I 655 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3110 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 2848 GGTACC G^GTACC
3868 GGTACC
AflII 3156 CTTAAG C^TTAAG
3949 CTTAAG
Aor51HI 438 AGCGCT AGC^GCT
4703 AGCGCT
ApaI 1245 GGGCCC GGGCC^C
3724 GGGCCC
Asp718I 2848 GGTACC G^GTACC
3868 GGTACC
BbvCI 3791 CCTCAGC CCTCAGC(-5/-2)
3814 CCTCAGC
BcgI 627 CGACAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3381 CGAGCCAGGTGC
BfrI 3156 CTTAAG C^TTAAG
3949 CTTAAG
BglI 399 GCCACGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
926 GCCCTCTGGGC
BsaWI 1299 TCCGGA [AT]^CCGG[AT]
2049 ACCGGA
BsmBI 1665 CGTCTC CGTCTC(1/5)
2393 GAGACG
BsmI 1882 GCATTC GAATGC(1/-1)
2818 GCATTC
Bsp1407I 4299 TGTACA T^GTACA
4446 TGTACA
BspHI 3402 TCATGA T^CATGA
5267 TCATGA
BspLU11I 2604 ACATGT A^CATGT
4253 ACATGT
BsrDI 1161 GCAATG GCAATG(2/0)
5512 GCAATG
BsrFI 1115 GCCGGT [GA]^CCGG[CT]
2851 ACCGGC
BsrGI 4299 TGTACA T^GTACA
4446 TGTACA
BstEII 2199 GGTCACC G^GT[ACGT]ACC
3765 GGTCACC
BstPI 2199 GGTCACC G^GT[ACGT]ACC
3765 GGTCACC
Cfr10I 1115 GCCGGT [GA]^CCGG[CT]
2851 ACCGGC
DraI 3000 TTTAAA TTT^AAA
4892 TTTAAA
DraIII 959 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
5316 CACTGTGTG
DrdI 1657 GACCGACGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1771 GACAGCCTGGTC
Eco47III 438 AGCGCT AGC^GCT
4703 AGCGCT
EcoO65I 2199 GGTCACC G^GT[ACGT]ACC
3765 GGTCACC
EcoRI 2123 GAATTC G^AATTC
3114 GAATTC
KpnI 2848 GGTACC GGTAC^C
3868 GGTACC
MluI 1662 ACGCGT A^CGCGT
2396 ACGCGT
NcoI 2424 CCATGG C^CATGG
3437 CCATGG
NheI 11 GCTAGC G^CTAGC
4271 GCTAGC
PshAI 1732 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5100 GACCTCTGTC
RcaI 3402 TCATGA T^CATGA
5267 TCATGA
ScaI 2090 AGTACT AGT^ACT
3844 AGTACT
SexAI 1023 ACCAGGT A^CC[AT]GGT
4325 ACCAGGT
SmlI 3156 CTTAAG C^T[CT][GA]AG
3949 CTTAAG
SspBI 4299 TGTACA T^GTACA
4446 TGTACA
SspI 1529 AATATT AAT^ATT
5504 AATATT
StuI 265 AGGCCT AGG^CCT
4763 AGGCCT
XcmI 93 CCAACGATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4467 CCACGTGGGTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI XmnI