##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccIII AgeI AscI AseI AsnI
AviII BbeI BclI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HpaI KasI
MamI MfeI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI PvuI RsrII SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1337 GACGTC GACGT^C
AclI 5631 AACGTT AA^CGTT
Alw44I 4017 GTGCAC G^TGCAC
AocI 3450 CCTCAGG CC^T[ACGT]AGG
ApaLI 4017 GTGCAC G^TGCAC
AspI 5854 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5073 GGATCC G^GATCC
BciVI 4239 GGATAC GTATCC(6/5)
BglII 5266 AGATCT A^GATCT
BlpI 4077 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4077 GCTCAGC GC^T[ACGT]AGC
BsiEI 6315 CGGCCG CG[GA][CT]^CG
BsmI 967 GAATGC GAATGC(1/-1)
BspHI 4690 TCATGA T^CATGA
BspLU11I 4851 ACATGT A^CATGT
Bsu36I 3450 CCTCAGG CC^T[ACGT]AGG
CelII 4077 GCTCAGC GC^T[ACGT]AGC
DrdI 20 GACTCGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 6315 CGGCCG C^GGCCG
EclXI 6315 CGGCCG C^GGCCG
Eco52I 6315 CGGCCG C^GGCCG
Eco81I 3450 CCTCAGG CC^T[ACGT]AGG
EcoNI 2509 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KspI 3800 CCGCGG CCGC^GG
MluI 1076 ACGCGT A^CGCGT
NcoI 4459 CCATGG C^CATGG
NdeI 5078 CATATG CA^TATG
PflFI 5854 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4594 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 5631 AACGTT AA^CGTT
RcaI 4690 TCATGA T^CATGA
SacII 3800 CCGCGG CCGC^GG
SalI 29 GTCGAC G^TCGAC
SpeI 3186 ACTAGT A^CTAGT
Tth111I 5854 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4594 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 1118 GGTACC G^GTACC
3818 GGTACC
AccII 1077 CGCG CG^CG
3801 CGCG
AflII 6217 CTTAAG C^TTAAG
6283 CTTAAG
Asp700I 5870 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6481 GAAGGGGTTC
Asp718I 1118 GGTACC G^GTACC
3818 GGTACC
AvrII 1717 CCTAGG C^CTAGG
2878 CCTAGG
BbrPI 594 CACGTG CAC^GTG
3068 CACGTG
BcgI 1753 CGATAAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1825 CGAAGGCACTGC
BfrI 6217 CTTAAG C^TTAAG
6283 CTTAAG
BlnI 1717 CCTAGG C^CTAGG
2878 CCTAGG
BsaAI 594 CACGTG [CT]AC^GT[GA]
3068 CACGTG
BsmBI 1664 GAGACG CGTCTC(1/5)
1778 GAGACG
Bsp1407I 5968 TGTACA T^GTACA
6530 TGTACA
BsrBI 3351 CCGCTC CCGCTC(-3/-3)
3280 GAGCGG
BsrGI 5968 TGTACA T^GTACA
6530 TGTACA
BstUI 1077 CGCG CG^CG
3801 CGCG
HincII 29 GTCGAC GT[CT]^[GA]AC
1630 GTCAAC
HindII 29 GTCGAC GT[CT]^[GA]AC
1630 GTCAAC
HindIII 3760 AAGCTT A^AGCTT
6568 AAGCTT
KpnI 1118 GGTACC GGTAC^C
3818 GGTACC
MvnI 1077 CGCG CG^CG
3801 CGCG
PmaCI 594 CACGTG CAC^GTG
3068 CACGTG
PmlI 594 CACGTG CAC^GTG
3068 CACGTG
PshAI 4135 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5816 GACCTAAGTC
SapI 4164 GAAGAGC GCTCTTC(1/4)
4511 GCTCTTC
SexAI 5619 ACCTGGT A^CC[AT]GGT
6457 ACCAGGT
SmaI 123 CCCGGG CCC^GGG
6322 CCCGGG
SspBI 5968 TGTACA T^GTACA
6530 TGTACA
XmaCI 123 CCCGGG C^CCGGG
6322 CCCGGG
XmaI 123 CCCGGG C^CCGGG
6322 CCCGGG
XmnI 5870 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6481 GAAGGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
Aor51HI ApaI ApoI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI Eam1105I EarI
Eco47III EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII