##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
AscI AseI AsnI Asp718I AspEI
BbeI BcgI BciVI BfrI BsaBI
BsmBI BspDI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I ClaI DraI
EagI Eam1105I EclXI Eco52I EcoRV
EcoT22I FseI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
PvuI SacII SalI ScaI SfiI
SfoI SfuI SmaI SnaBI SpeI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 2455 GTCTAC GT^[AC][GT]AC
AccII 1345 CGCG CG^CG
AccIII 1808 TCCGGA T^CCGGA
AclI 2650 AACGTT AA^CGTT
AcyI 444 GACGCC G[GA]^CG[CT]C
AflIII 4081 ACACGT A^C[GA][CT]GT
AspI 1801 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1984 TGCGCA TGC^GCA
AvrII 2713 CCTAGG C^CTAGG
BbrPI 4311 CACGTG CAC^GTG
BclI 4103 TGATCA T^GATCA
BlnI 2713 CCTAGG C^CTAGG
BlpI 2331 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2331 GCTGAGC GC^T[ACGT]AGC
BsaAI 4311 CACGTG [CT]AC^GT[GA]
BsaHI 444 GACGCC G[GA]^CG[CT]C
BseAI 1808 TCCGGA T^CCGGA
BsiEI 1949 CGACCG CG[GA][CT]^CG
BsiWI 1347 CGTACG C^GTACG
BsmI 2560 GAATGC GAATGC(1/-1)
Bsp1407I 2910 TGTACA T^GTACA
BspEI 1808 TCCGGA T^CCGGA
BspHI 1094 TCATGA T^CATGA
BsrBI 803 CCGCTC CCGCTC(-3/-3)
BsrDI 2939 CATTGC GCAATG(2/0)
BsrGI 2910 TGTACA T^GTACA
BstUI 1345 CGCG CG^CG
CelII 2331 GCTGAGC GC^T[ACGT]AGC
CpoI 714 CGGACCG CG^G[AT]CCG
FbaI 4103 TGATCA T^GATCA
FspI 1984 TGCGCA TGC^GCA
Hin1I 444 GACGCC G[GA]^CG[CT]C
HincII 2660 GTTAAC GT[CT]^[GA]AC
HindII 2660 GTTAAC GT[CT]^[GA]AC
HpaI 2660 GTTAAC GTT^AAC
MroI 1808 TCCGGA T^CCGGA
MvnI 1345 CGCG CG^CG
NheI 4071 GCTAGC G^CTAGC
PflFI 1801 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4311 CACGTG CAC^GTG
PmlI 4311 CACGTG CAC^GTG
PshAI 1581 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2650 AACGTT AA^CGTT
RcaI 1094 TCATGA T^CATGA
RsrII 714 CGGACCG CG^G[AT]CCG
SapI 502 GAAGAGC GCTCTTC(1/4)
SbfI 3441 CCTGCAGG CCTGCA^GG
SexAI 1802 ACCTGGT A^CC[AT]GGT
SgrAI 2626 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 3441 CCTGCAGG CCTGCA^GG
SspBI 2910 TGTACA T^GTACA
Tth111I 1801 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3867 TCTAGA T^CTAGA
XcmI 519 CCACAAATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1051 GTGCAC G^TGCAC
2472 GTGCAC
Aor51HI 814 AGCGCT AGC^GCT
4002 AGCGCT
ApaI 1716 GGGCCC GGGCC^C
2174 GGGCCC
ApaLI 1051 GTGCAC G^TGCAC
2472 GTGCAC
BamHI 2837 GGATCC G^GATCC
4130 GGATCC
BglI 1429 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1709 GCCTTGGGGGC
BglII 312 AGATCT A^GATCT
2498 AGATCT
BsaWI 1577 ACCGGA [AT]^CCGG[AT]
1808 TCCGGA
BsrFI 2141 GCCGGC [GA]^CCGG[CT]
2627 GCCGGC
BstEII 3352 GGTGACC G^GT[ACGT]ACC
3876 GGTTACC
BstPI 3352 GGTGACC G^GT[ACGT]ACC
3876 GGTTACC
Cfr10I 2141 GCCGGC [GA]^CCGG[CT]
2627 GCCGGC
DrdI 127 GACATCGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2539 GACACCACAGTC
Eco47III 814 AGCGCT AGC^GCT
4002 AGCGCT
EcoNI 2889 CCTCCGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3887 CCTCCCTGAGG
EcoO65I 3352 GGTGACC G^GT[ACGT]ACC
3876 GGTTACC
HgaI 444 GACGC GACGC(5/10)
3284 GCGTC
HindIII 721 AAGCTT A^AGCTT
3918 AAGCTT
NaeI 2141 GCCGGC GCC^GGC
2627 GCCGGC
NcoI 1127 CCATGG C^CATGG
1668 CCATGG
NgoMIV 2141 GCCGGC G^CCGGC
2627 GCCGGC
PpuMI 1586 GGGTCCC [GA]G^G[AT]CC[CT]
2117 GGGACCT
SphI 1499 GCATGC GCATG^C
2234 GCATGC
SspI 3014 AATATT AAT^ATT
4362 AATATT
StuI 947 AGGCCT AGG^CCT
3123 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI