##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AclI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbrPI BbvCI BcgI
BlnI BsaAI BsaBI BsgI BsiEI
BsiWI BspDI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI FseI
FspI HincII HindII HpaI KasI
KpnI KspI MamI MluI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2254 GACGTC GACGT^C
AccI 2229 GTCTAC GT^[AC][GT]AC
AccII 48 CGCG CG^CG
AccIII 2968 TCCGGA T^CCGGA
AflII 5120 CTTAAG C^TTAAG
AflIII 2637 ACATGT A^C[GA][CT]GT
AgeI 4200 ACCGGT A^CCGGT
AspI 366 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 302 TGATCA T^GATCA
BcnI 5912 CCGGG CC^[GC]GG
BfrI 5120 CTTAAG C^TTAAG
BglII 5851 AGATCT A^GATCT
BsaI 769 GGTCTC GGTCTC(1/5)
BseAI 2968 TCCGGA T^CCGGA
BsmBI 1158 GAGACG CGTCTC(1/5)
BspEI 2968 TCCGGA T^CCGGA
BspLU11I 2637 ACATGT A^CATGT
BstUI 48 CGCG CG^CG
DrdI 621 GACAGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 222 GATATC GAT^ATC
FbaI 302 TGATCA T^GATCA
HindIII 5412 AAGCTT A^AGCTT
MfeI 2681 CAATTG C^AATTG
MroI 2968 TCCGGA T^CCGGA
MunI 2681 CAATTG C^AATTG
MvnI 48 CGCG CG^CG
NciI 5912 CCGGG CC^[GC]GG
NcoI 5712 CCATGG C^CATGG
NheI 3567 GCTAGC G^CTAGC
PflFI 366 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4200 ACCGGT A^CCGGT
PvuII 4400 CAGCTG CAG^CTG
SapI 4174 GCTCTTC GCTCTTC(1/4)
SbfI 74 CCTGCAGG CCTGCA^GG
Sse8387I 74 CCTGCAGG CCTGCA^GG
StuI 3459 AGGCCT AGG^CCT
SwaI 2168 ATTTAAAT ATTT^AAAT
Tth111I 366 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1257 GTGCAC G^TGCAC
1581 GTGCAC
AlwNI 3559 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
4571 CAGTATCTG
ApaI 4869 GGGCCC GGGCC^C
5914 GGGCCC
ApaLI 1257 GTGCAC G^TGCAC
1581 GTGCAC
BamHI 3047 GGATCC G^GATCC
5087 GGATCC
BbsI 2850 GTCTTC GAAGAC(2/6)
4390 GTCTTC
BciVI 5130 GGATAC GTATCC(6/5)
5240 GGATAC
BglI 3576 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5646 GCCAGCCAGGC
BpmI 2876 CTCCAG CTGGAG(16/14)
2990 CTCCAG
BpuAI 2850 GTCTTC GAAGAC(2/6)
4390 GTCTTC
BsaWI 2968 TCCGGA [AT]^CCGG[AT]
4200 ACCGGT
BspHI 3180 TCATGA T^CATGA
3712 TCATGA
BsrDI 2047 CATTGC GCAATG(2/0)
759 GCAATG
BsrFI 696 ACCGGC [GA]^CCGG[CT]
4200 ACCGGT
BssSI 3149 CTCGTG CACGAG(-5/-1)
3643 CTCGTG
BstAPI 1526 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6133 GCAAGTACTGC
Cfr10I 696 ACCGGC [GA]^CCGG[CT]
4200 ACCGGT
DsaI 5332 CCGTGG C^C[GA][CT]GG
5712 CCATGG
FauI 3534 CCCGC CCCGC(4/6)
5614 CCCGC
PshAI 1297 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5602 GACCATTGTC
PstI 75 CTGCAG CTGCA^G
688 CTGCAG
RcaI 3180 TCATGA T^CATGA
3712 TCATGA
ScaI 408 AGTACT AGT^ACT
6136 AGTACT
SphI 3638 GCATGC GCATG^C
4271 GCATGC
XcmI 590 CCAGCCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2962 CCACCATCCGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AocI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbvI BfaI BlpI BmyI Bpu1102I
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI