##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BciVI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiWI
BsoBI BspDI BspEI BspLU11I BsrFI
Bst1107I BstBI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
Eco47III Eco81I EcoRI FseI FspI
HaeII Hin1I HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 653 AACGTT AA^CGTT
AhdI 2653 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 283 GGGCCC GGGCC^C
AseI 3055 ATTAAT AT^TAAT
AsnI 3055 ATTAAT AT^TAAT
AspEI 2653 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 455 GAGCTC G[AT]GC[AT]^C
BcgI 3962 GCAGAAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4016 TGATCA T^GATCA
BglI 62 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2781 GAGACC GGTCTC(1/5)
BsiEI 163 CGGCCG CG[GA][CT]^CG
BsiHKAI 455 GAGCTC G[AT]GC[AT]^C
BsmBI 984 GAGACG CGTCTC(1/5)
Bsp1407I 3438 TGTACA T^GTACA
BspHI 3024 TCATGA T^CATGA
BsrBI 31 CCGCTC CCGCTC(-3/-3)
BsrDI 1850 GCAATG GCAATG(2/0)
BsrGI 3438 TGTACA T^GTACA
BssHII 7 GCGCGC G^CGCGC
BstEII 889 GGTTACC G^GT[ACGT]ACC
BstPI 889 GGTTACC G^GT[ACGT]ACC
EagI 163 CGGCCG C^GGCCG
Eam1105I 2653 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 163 CGGCCG C^GGCCG
Eco52I 163 CGGCCG C^GGCCG
EcoNI 2118 CCTTATAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 889 GGTTACC G^GT[ACGT]ACC
EcoRV 3922 GATATC GAT^ATC
EcoT22I 2246 ATGCAT ATGCA^T
FbaI 4016 TGATCA T^GATCA
HgaI 2217 GCGTC GACGC(5/10)
HincII 3249 GTCAAC GT[CT]^[GA]AC
HindII 3249 GTCAAC GT[CT]^[GA]AC
HindIII 1706 AAGCTT A^AGCTT
MfeI 836 CAATTG C^AATTG
MunI 836 CAATTG C^AATTG
NcoI 66 CCATGG C^CATGG
NsiI 2246 ATGCAT ATGCA^T
PflMI 1883 CCATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3055 ATTAAT AT^TAAT
Psp1406I 653 AACGTT AA^CGTT
RcaI 3024 TCATGA T^CATGA
SacI 455 GAGCTC GAGCT^C
SapI 695 GAAGAGC GCTCTTC(1/4)
SexAI 3322 ACCTGGT A^CC[AT]GGT
SspBI 3438 TGTACA T^GTACA
Van91I 1883 CCATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3276 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 359 CTTAAG C^TTAAG
2868 CTTAAG
BalI 1059 TGGCCA TGG^CCA
3447 TGGCCA
BanI 2188 GGTGCC G^G[CT][GA]CC
4023 GGTGCC
BcnI 343 CCGGG CC^[GC]GG
936 CCCGG
BfrI 359 CTTAAG C^TTAAG
2868 CTTAAG
BglII 1368 AGATCT A^GATCT
1450 AGATCT
BsaBI 548 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1541 GATGAAAATC
BsmI 931 GCATTC GAATGC(1/-1)
2248 GCATTC
BssSI 1088 CACGAG CACGAG(-5/-1)
3970 CTCGTG
BstXI 84 CCACAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2655 CCAGTGGTCTGG
DsaI 66 CCATGG C^C[GA][CT]GG
90 CCGTGG
EcoT14I 66 CCATGG C^C[AT][AT]GG
4027 CCAAGG
HapII 343 CCGG C^CGG
937 CCGG
HpaII 343 CCGG C^CGG
937 CCGG
MaeII 540 ACGT A^CGT
654 ACGT
MamI 548 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1541 GATGAAAATC
MluNI 1059 TGGCCA TGG^CCA
3447 TGGCCA
MscI 1059 TGGCCA TGG^CCA
3447 TGGCCA
MspI 343 CCGG C^CGG
937 CCGG
NciI 343 CCGGG CC^[GC]GG
936 CCCGG
NspI 1878 ACATGC [GA]CATG^[CT]
2039 GCATGT
PpuMI 78 AGGACCC [GA]G^G[AT]CC[CT]
573 AGGACCT
ScaI 1950 AGTACT AGT^ACT
3998 AGTACT
SmlI 359 CTTAAG C^T[CT][GA]AG
2868 CTTAAG
StyI 66 CCATGG C^C[AT][AT]GG
4027 CCAAGG
Tsp45I 2219 GTCAC ^GT[GC]AC
3136 GTGAC
XcmI 1062 CCATGAAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1251 CCATGAGGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII XmnI