################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiWI BsoBI BspDI BspEI BspLU11I BsrFI Bst1107I BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI Eco47III Eco81I EcoRI FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AclI 653 AACGTT AA^CGTT AhdI 2653 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 283 GGGCCC GGGCC^C AseI 3055 ATTAAT AT^TAAT AsnI 3055 ATTAAT AT^TAAT AspEI 2653 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 455 GAGCTC G[AT]GC[AT]^C BcgI 3962 GCAGAAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 4016 TGATCA T^GATCA BglI 62 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 2781 GAGACC GGTCTC(1/5) BsiEI 163 CGGCCG CG[GA][CT]^CG BsiHKAI 455 GAGCTC G[AT]GC[AT]^C BsmBI 984 GAGACG CGTCTC(1/5) Bsp1407I 3438 TGTACA T^GTACA BspHI 3024 TCATGA T^CATGA BsrBI 31 CCGCTC CCGCTC(-3/-3) BsrDI 1850 GCAATG GCAATG(2/0) BsrGI 3438 TGTACA T^GTACA BssHII 7 GCGCGC G^CGCGC BstEII 889 GGTTACC G^GT[ACGT]ACC BstPI 889 GGTTACC G^GT[ACGT]ACC EagI 163 CGGCCG C^GGCCG Eam1105I 2653 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 163 CGGCCG C^GGCCG Eco52I 163 CGGCCG C^GGCCG EcoNI 2118 CCTTATAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 889 GGTTACC G^GT[ACGT]ACC EcoRV 3922 GATATC GAT^ATC EcoT22I 2246 ATGCAT ATGCA^T FbaI 4016 TGATCA T^GATCA HgaI 2217 GCGTC GACGC(5/10) HincII 3249 GTCAAC GT[CT]^[GA]AC HindII 3249 GTCAAC GT[CT]^[GA]AC HindIII 1706 AAGCTT A^AGCTT MfeI 836 CAATTG C^AATTG MunI 836 CAATTG C^AATTG NcoI 66 CCATGG C^CATGG NsiI 2246 ATGCAT ATGCA^T PflMI 1883 CCATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 3055 ATTAAT AT^TAAT Psp1406I 653 AACGTT AA^CGTT RcaI 3024 TCATGA T^CATGA SacI 455 GAGCTC GAGCT^C SapI 695 GAAGAGC GCTCTTC(1/4) SexAI 3322 ACCTGGT A^CC[AT]GGT SspBI 3438 TGTACA T^GTACA Van91I 1883 CCATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3276 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AflII 359 CTTAAG C^TTAAG 2868 CTTAAG BalI 1059 TGGCCA TGG^CCA 3447 TGGCCA BanI 2188 GGTGCC G^G[CT][GA]CC 4023 GGTGCC BcnI 343 CCGGG CC^[GC]GG 936 CCCGG BfrI 359 CTTAAG C^TTAAG 2868 CTTAAG BglII 1368 AGATCT A^GATCT 1450 AGATCT BsaBI 548 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1541 GATGAAAATC BsmI 931 GCATTC GAATGC(1/-1) 2248 GCATTC BssSI 1088 CACGAG CACGAG(-5/-1) 3970 CTCGTG BstXI 84 CCACAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2655 CCAGTGGTCTGG DsaI 66 CCATGG C^C[GA][CT]GG 90 CCGTGG EcoT14I 66 CCATGG C^C[AT][AT]GG 4027 CCAAGG HapII 343 CCGG C^CGG 937 CCGG HpaII 343 CCGG C^CGG 937 CCGG MaeII 540 ACGT A^CGT 654 ACGT MamI 548 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1541 GATGAAAATC MluNI 1059 TGGCCA TGG^CCA 3447 TGGCCA MscI 1059 TGGCCA TGG^CCA 3447 TGGCCA MspI 343 CCGG C^CGG 937 CCGG NciI 343 CCGGG CC^[GC]GG 936 CCCGG NspI 1878 ACATGC [GA]CATG^[CT] 2039 GCATGT PpuMI 78 AGGACCC [GA]G^G[AT]CC[CT] 573 AGGACCT ScaI 1950 AGTACT AGT^ACT 3998 AGTACT SmlI 359 CTTAAG C^T[CT][GA]AG 2868 CTTAAG StyI 66 CCATGG C^C[AT][AT]GG 4027 CCAAGG Tsp45I 2219 GTCAC ^GT[GC]AC 3136 GTGAC XcmI 1062 CCATGAAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1251 CCATGAGGCAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI