################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AscI Asp700I Asp718I AviII BclI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspEI BsrBI BssSI BstBI CpoI EagI EclXI Eco52I EcoNI EcoRI EcoRV FbaI FseI FspI HindIII HpaI KpnI KspI MluI MroI NdeI NotI NruI NspV PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfuI SgrAI SmaI SnaBI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AcyI 3065 GGCGCC G[GA]^CG[CT]C AflII 4537 CTTAAG C^TTAAG AhdI 3020 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 60 GTGCAC G^TGCAC ApaI 1445 GGGCCC GGGCC^C ApaLI 60 GTGCAC G^TGCAC AseI 4979 ATTAAT AT^TAAT AsnI 4979 ATTAAT AT^TAAT AspEI 3020 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 344 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 232 TGGCCA TGG^CCA BamHI 4001 GGATCC G^GATCC BbeI 3065 GGCGCC GGCGC^C BbrPI 3178 CACGTG CAC^GTG BcgI 3654 CGACCTATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 4537 CTTAAG C^TTAAG BglI 2793 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 3065 GGCGCC G[GA]^CG[CT]C Bsp1407I 4344 TGTACA T^GTACA BspDI 3794 ATCGAT AT^CGAT BspLU11I 419 ACATGT A^CATGT BsrFI 1787 GCCGGC [GA]^CCGG[CT] BsrGI 4344 TGTACA T^GTACA Bst1107I 1934 GTATAC GTA^TAC BstXI 2567 CCACAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1934 GTATAC GTA^TAC Cfr10I 1787 GCCGGC [GA]^CCGG[CT] ClaI 3794 ATCGAT AT^CGAT DrdI 705 GACATGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3020 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Hin1I 3065 GGCGCC G[GA]^CG[CT]C HincII 737 GTTGAC GT[CT]^[GA]AC HindII 737 GTTGAC GT[CT]^[GA]AC KasI 3065 GGCGCC G^GCGCC MfeI 4177 CAATTG C^AATTG MluNI 232 TGGCCA TGG^CCA MscI 232 TGGCCA TGG^CCA MunI 4177 CAATTG C^AATTG NaeI 1787 GCCGGC GCC^GGC NarI 3065 GGCGCC GG^CGCC NcoI 1541 CCATGG C^CATGG NgoMIV 1787 GCCGGC G^CCGGC NheI 2364 GCTAGC G^CTAGC PacI 2454 TTAATTAA TTAAT^TAA PflFI 344 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2291 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3178 CACGTG CAC^GTG PmlI 3178 CACGTG CAC^GTG PshBI 4979 ATTAAT AT^TAAT SacI 324 GAGCTC GAGCT^C SbfI 4548 CCTGCAGG CCTGCA^GG ScaI 4527 AGTACT AGT^ACT SfoI 3065 GGCGCC GGC^GCC Sse8387I 4548 CCTGCAGG CCTGCA^GG SspBI 4344 TGTACA T^GTACA SspI 3257 AATATT AAT^ATT SwaI 532 ATTTAAAT ATTT^AAAT Tth111I 344 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2291 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccII 3367 CGCG CG^CG 3369 CGCG AflIII 419 ACATGT A^C[GA][CT]GT 3177 ACACGT AocI 1794 CCTCAGG CC^T[ACGT]AGG 3647 CCTGAGG Aor51HI 1516 AGCGCT AGC^GCT 2656 AGCGCT AvaI 321 CTCGAG C^[CT]CG[GA]G 2611 CTCGAG AvrII 1805 CCTAGG C^CTAGG 4303 CCTAGG BbvCI 366 GCTGAGG CCTCAGC(-5/-2) 4898 GCTGAGG BciVI 4729 GGATAC GTATCC(6/5) 5022 GGATAC BlnI 1805 CCTAGG C^CTAGG 4303 CCTAGG BsaAI 3178 CACGTG [CT]AC^GT[GA] 3317 TACGTG BsaI 1840 GAGACC GGTCTC(1/5) 892 GGTCTC BseRI 1792 CTCCTC GAGGAG(10/8) 2729 CTCCTC BsoBI 321 CTCGAG C^[CT]CG[GA]G 2611 CTCGAG BspHI 196 TCATGA T^CATGA 1247 TCATGA BssHII 3366 GCGCGC G^CGCGC 3368 GCGCGC BstEII 173 GGTGACC G^GT[ACGT]ACC 2682 GGTTACC BstPI 173 GGTGACC G^GT[ACGT]ACC 2682 GGTTACC BstUI 3367 CGCG CG^CG 3369 CGCG Bsu36I 1794 CCTCAGG CC^T[ACGT]AGG 3647 CCTGAGG DraIII 2292 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG 3155 CACCATGTG EaeI 232 TGGCCA [CT]^GGCC[GA] 4163 TGGCCG Eco47III 1516 AGCGCT AGC^GCT 2656 AGCGCT Eco81I 1794 CCTCAGG CC^T[ACGT]AGG 3647 CCTGAGG EcoO65I 173 GGTGACC G^GT[ACGT]ACC 2682 GGTTACC EcoT22I 2787 ATGCAT ATGCA^T 3912 ATGCAT FauI 2157 CCCGC CCCGC(4/6) 2186 CCCGC HgaI 690 GACGC GACGC(5/10) 606 GCGTC MvnI 3367 CGCG CG^CG 3369 CGCG NsiI 2787 ATGCAT ATGCA^T 3912 ATGCAT PaeR7I 321 CTCGAG C^TCGAG 2611 CTCGAG PvuII 991 CAGCTG CAG^CTG 2243 CAGCTG RcaI 196 TCATGA T^CATGA 1247 TCATGA SapI 1235 GAAGAGC GCTCTTC(1/4) 1512 GAAGAGC SpeI 2988 ACTAGT A^CTAGT 4662 ACTAGT StuI 3002 AGGCCT AGG^CCT 3644 AGGCCT XhoI 321 CTCGAG C^TCGAG 2611 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII