##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI Asp700I Asp718I AviII BclI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspEI BsrBI BssSI BstBI
CpoI EagI EclXI Eco52I EcoNI
EcoRI EcoRV FbaI FseI FspI
HindIII HpaI KpnI KspI MluI
MroI NdeI NotI NruI NspV
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RsrII SacII SalI SexAI
SfiI SfuI SgrAI SmaI SnaBI
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AcyI 3065 GGCGCC G[GA]^CG[CT]C
AflII 4537 CTTAAG C^TTAAG
AhdI 3020 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 60 GTGCAC G^TGCAC
ApaI 1445 GGGCCC GGGCC^C
ApaLI 60 GTGCAC G^TGCAC
AseI 4979 ATTAAT AT^TAAT
AsnI 4979 ATTAAT AT^TAAT
AspEI 3020 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 344 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 232 TGGCCA TGG^CCA
BamHI 4001 GGATCC G^GATCC
BbeI 3065 GGCGCC GGCGC^C
BbrPI 3178 CACGTG CAC^GTG
BcgI 3654 CGACCTATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4537 CTTAAG C^TTAAG
BglI 2793 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 3065 GGCGCC G[GA]^CG[CT]C
Bsp1407I 4344 TGTACA T^GTACA
BspDI 3794 ATCGAT AT^CGAT
BspLU11I 419 ACATGT A^CATGT
BsrFI 1787 GCCGGC [GA]^CCGG[CT]
BsrGI 4344 TGTACA T^GTACA
Bst1107I 1934 GTATAC GTA^TAC
BstXI 2567 CCACAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1934 GTATAC GTA^TAC
Cfr10I 1787 GCCGGC [GA]^CCGG[CT]
ClaI 3794 ATCGAT AT^CGAT
DrdI 705 GACATGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3020 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 3065 GGCGCC G[GA]^CG[CT]C
HincII 737 GTTGAC GT[CT]^[GA]AC
HindII 737 GTTGAC GT[CT]^[GA]AC
KasI 3065 GGCGCC G^GCGCC
MfeI 4177 CAATTG C^AATTG
MluNI 232 TGGCCA TGG^CCA
MscI 232 TGGCCA TGG^CCA
MunI 4177 CAATTG C^AATTG
NaeI 1787 GCCGGC GCC^GGC
NarI 3065 GGCGCC GG^CGCC
NcoI 1541 CCATGG C^CATGG
NgoMIV 1787 GCCGGC G^CCGGC
NheI 2364 GCTAGC G^CTAGC
PacI 2454 TTAATTAA TTAAT^TAA
PflFI 344 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2291 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3178 CACGTG CAC^GTG
PmlI 3178 CACGTG CAC^GTG
PshBI 4979 ATTAAT AT^TAAT
SacI 324 GAGCTC GAGCT^C
SbfI 4548 CCTGCAGG CCTGCA^GG
ScaI 4527 AGTACT AGT^ACT
SfoI 3065 GGCGCC GGC^GCC
Sse8387I 4548 CCTGCAGG CCTGCA^GG
SspBI 4344 TGTACA T^GTACA
SspI 3257 AATATT AAT^ATT
SwaI 532 ATTTAAAT ATTT^AAAT
Tth111I 344 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2291 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 3367 CGCG CG^CG
3369 CGCG
AflIII 419 ACATGT A^C[GA][CT]GT
3177 ACACGT
AocI 1794 CCTCAGG CC^T[ACGT]AGG
3647 CCTGAGG
Aor51HI 1516 AGCGCT AGC^GCT
2656 AGCGCT
AvaI 321 CTCGAG C^[CT]CG[GA]G
2611 CTCGAG
AvrII 1805 CCTAGG C^CTAGG
4303 CCTAGG
BbvCI 366 GCTGAGG CCTCAGC(-5/-2)
4898 GCTGAGG
BciVI 4729 GGATAC GTATCC(6/5)
5022 GGATAC
BlnI 1805 CCTAGG C^CTAGG
4303 CCTAGG
BsaAI 3178 CACGTG [CT]AC^GT[GA]
3317 TACGTG
BsaI 1840 GAGACC GGTCTC(1/5)
892 GGTCTC
BseRI 1792 CTCCTC GAGGAG(10/8)
2729 CTCCTC
BsoBI 321 CTCGAG C^[CT]CG[GA]G
2611 CTCGAG
BspHI 196 TCATGA T^CATGA
1247 TCATGA
BssHII 3366 GCGCGC G^CGCGC
3368 GCGCGC
BstEII 173 GGTGACC G^GT[ACGT]ACC
2682 GGTTACC
BstPI 173 GGTGACC G^GT[ACGT]ACC
2682 GGTTACC
BstUI 3367 CGCG CG^CG
3369 CGCG
Bsu36I 1794 CCTCAGG CC^T[ACGT]AGG
3647 CCTGAGG
DraIII 2292 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3155 CACCATGTG
EaeI 232 TGGCCA [CT]^GGCC[GA]
4163 TGGCCG
Eco47III 1516 AGCGCT AGC^GCT
2656 AGCGCT
Eco81I 1794 CCTCAGG CC^T[ACGT]AGG
3647 CCTGAGG
EcoO65I 173 GGTGACC G^GT[ACGT]ACC
2682 GGTTACC
EcoT22I 2787 ATGCAT ATGCA^T
3912 ATGCAT
FauI 2157 CCCGC CCCGC(4/6)
2186 CCCGC
HgaI 690 GACGC GACGC(5/10)
606 GCGTC
MvnI 3367 CGCG CG^CG
3369 CGCG
NsiI 2787 ATGCAT ATGCA^T
3912 ATGCAT
PaeR7I 321 CTCGAG C^TCGAG
2611 CTCGAG
PvuII 991 CAGCTG CAG^CTG
2243 CAGCTG
RcaI 196 TCATGA T^CATGA
1247 TCATGA
SapI 1235 GAAGAGC GCTCTTC(1/4)
1512 GAAGAGC
SpeI 2988 ACTAGT A^CTAGT
4662 ACTAGT
StuI 3002 AGGCCT AGG^CCT
3644 AGGCCT
XhoI 321 CTCGAG C^TCGAG
2611 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII