##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AscI Asp718I AvrII BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BseAI BsiWI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
CpoI EcoO65I EcoRI FbaI FseI
HpaI KpnI MluI MroI NdeI
NheI NotI NruI NspV PacI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SnaBI Sse8387I StuI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 4146 GACGTC GACGT^C
AccI 3284 GTAGAC GT^[AC][GT]AC
AocI 4864 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2849 AGCGCT AGC^GCT
AviII 1213 TGCGCA TGC^GCA
BamHI 2466 GGATCC G^GATCC
BbrPI 1814 CACGTG CAC^GTG
BglI 2018 GCCAGTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 590 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3273 GGTCTC GGTCTC(1/5)
BsaWI 961 ACCGGA [AT]^CCGG[AT]
BsgI 3452 CTGCAC GTGCAG(16/14)
BsiEI 161 CGGCCG CG[GA][CT]^CG
BssSI 1805 CACGAG CACGAG(-5/-1)
BstAPI 2943 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1186 CCAAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4864 CCTTAGG CC^T[ACGT]AGG
DrdI 423 GACCTGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 161 CGGCCG C^GGCCG
EclXI 161 CGGCCG C^GGCCG
Eco47III 2849 AGCGCT AGC^GCT
Eco52I 161 CGGCCG C^GGCCG
Eco81I 4864 CCTTAGG CC^T[ACGT]AGG
EcoT22I 2893 ATGCAT ATGCA^T
FspI 1213 TGCGCA TGC^GCA
HincII 4683 GTCAAC GT[CT]^[GA]AC
HindII 4683 GTCAAC GT[CT]^[GA]AC
HindIII 3244 AAGCTT A^AGCTT
MamI 590 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 102 CAATTG C^AATTG
MunI 102 CAATTG C^AATTG
NsiI 2893 ATGCAT ATGCA^T
PaeR7I 46 CTCGAG C^TCGAG
PmaCI 1814 CACGTG CAC^GTG
PmlI 1814 CACGTG CAC^GTG
SexAI 1285 ACCAGGT A^CC[AT]GGT
SgrAI 236 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1688 CCCGGG CCC^GGG
SpeI 1316 ACTAGT A^CTAGT
SphI 3979 GCATGC GCATG^C
SspI 664 AATATT AAT^ATT
XhoI 46 CTCGAG C^TCGAG
XmaCI 1688 CCCGGG C^CCGGG
XmaI 1688 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AhdI 1507 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3480 GACCCCAAGTC
Alw44I 797 GTGCAC G^TGCAC
1819 GTGCAC
ApaI 1591 GGGCCC GGGCC^C
3847 GGGCCC
ApaLI 797 GTGCAC G^TGCAC
1819 GTGCAC
AseI 3065 ATTAAT AT^TAAT
4269 ATTAAT
AsnI 3065 ATTAAT AT^TAAT
4269 ATTAAT
AspEI 1507 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3480 GACCCCAAGTC
AspI 2391 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3268 GACAGGGTC
BbeI 198 GGCGCC GGCGC^C
234 GGCGCC
BbvCI 1466 CCTCAGC CCTCAGC(-5/-2)
3915 GCTGAGG
BglII 2037 AGATCT A^GATCT
4803 AGATCT
BsaAI 1814 CACGTG [CT]AC^GT[GA]
3317 TACGTG
BsmBI 870 CGTCTC CGTCTC(1/5)
3821 CGTCTC
BspDI 1359 ATCGAT AT^CGAT
4176 ATCGAT
BspHI 4313 TCATGA T^CATGA
4882 TCATGA
BspLU11I 1300 ACATGT A^CATGT
2083 ACATGT
BsrDI 2948 CATTGC GCAATG(2/0)
763 GCAATG
ClaI 1359 ATCGAT AT^CGAT
4176 ATCGAT
Eam1105I 1507 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3480 GACCCCAAGTC
EcoNI 3947 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4214 CCTCTCAGAGG
EcoRV 1608 GATATC GAT^ATC
4852 GATATC
KasI 198 GGCGCC G^GCGCC
234 GGCGCC
KspI 23 CCGCGG CCGC^GG
275 CCGCGG
NarI 198 GGCGCC GG^CGCC
234 GGCGCC
PflFI 2391 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3268 GACAGGGTC
PshBI 3065 ATTAAT AT^TAAT
4269 ATTAAT
RcaI 4313 TCATGA T^CATGA
4882 TCATGA
SacI 1485 GAGCTC GAGCT^C
3684 GAGCTC
SacII 23 CCGCGG CCGC^GG
275 CCGCGG
SapI 1713 GAAGAGC GCTCTTC(1/4)
1775 GAAGAGC
SfoI 198 GGCGCC GGC^GCC
234 GGCGCC
Tth111I 2391 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3268 GACAGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI