##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AccI AclI AscI AseI
AsnI Asp718I BclI BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EcoRV EcoT22I FbaI FseI
HindIII KpnI MamI MfeI MluI
MunI NcoI NdeI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SfiI SfuI SnaBI
SspBI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 554 GACGTC GACGT^C
AccIII 33 TCCGGA T^CCGGA
AgeI 1989 ACCGGT A^CCGGT
AhdI 2297 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2396 CCTAAGG CC^T[ACGT]AGG
Aor51HI 27 AGCGCT AGC^GCT
Asp700I 3506 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2297 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2897 TGCGCA TGC^GCA
BamHI 3774 GGATCC G^GATCC
BbsI 4592 GTCTTC GAAGAC(2/6)
BglII 3710 AGATCT A^GATCT
BpuAI 4592 GTCTTC GAAGAC(2/6)
BsaI 3393 GAGACC GGTCTC(1/5)
BseAI 33 TCCGGA T^CCGGA
BspEI 33 TCCGGA T^CCGGA
BsrDI 3861 CATTGC GCAATG(2/0)
BssSI 386 CTCGTG CACGAG(-5/-1)
BstEII 3611 GGTCACC G^GT[ACGT]ACC
BstPI 3611 GGTCACC G^GT[ACGT]ACC
Bsu36I 2396 CCTAAGG CC^T[ACGT]AGG
DrdI 1389 GACCTCGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 129 CGGCCG C^GGCCG
Eam1105I 2297 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 129 CGGCCG C^GGCCG
Eco47III 27 AGCGCT AGC^GCT
Eco52I 129 CGGCCG C^GGCCG
Eco81I 2396 CCTAAGG CC^T[ACGT]AGG
EcoO65I 3611 GGTCACC G^GT[ACGT]ACC
EcoRI 3647 GAATTC G^AATTC
FspI 2897 TGCGCA TGC^GCA
HincII 1819 GTTAAC GT[CT]^[GA]AC
HindII 1819 GTTAAC GT[CT]^[GA]AC
HpaI 1819 GTTAAC GTT^AAC
KspI 276 CCGCGG CCGC^GG
MroI 33 TCCGGA T^CCGGA
NheI 350 GCTAGC G^CTAGC
NotI 128 GCGGCCGC GC^GGCCGC
PinAI 1989 ACCGGT A^CCGGT
SacII 276 CCGCGG CCGC^GG
SbfI 2026 CCTGCAGG CCTGCA^GG
SgrAI 531 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3451 ACTAGT A^CTAGT
Sse8387I 2026 CCTGCAGG CCTGCA^GG
XmnI 3506 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 3141 GAATTT [GA]^AATT[CT]
3647 GAATTC
AflIII 2301 ACGTGT A^C[GA][CT]GT
4935 ACGTGT
ApoI 3141 GAATTT [GA]^AATT[CT]
3647 GAATTC
AvrII 3088 CCTAGG C^CTAGG
4332 CCTAGG
BalI 2004 TGGCCA TGG^CCA
4295 TGGCCA
BbeI 175 GGCGCC GGCGC^C
2125 GGCGCC
BbrPI 2300 CACGTG CAC^GTG
4934 CACGTG
BcgI 436 CGAGCTGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2562 CGAAGATTCTGC
BciVI 1663 GGATAC GTATCC(6/5)
3992 GGATAC
BlnI 3088 CCTAGG C^CTAGG
4332 CCTAGG
BsaAI 2300 CACGTG [CT]AC^GT[GA]
4934 CACGTG
BsiEI 129 CGGCCG CG[GA][CT]^CG
261 CGACCG
BsrBI 629 GAGCGG CCGCTC(-3/-3)
1325 GAGCGG
DraIII 1029 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2636 CACCAAGTG
KasI 175 GGCGCC G^GCGCC
2125 GGCGCC
MluNI 2004 TGGCCA TGG^CCA
4295 TGGCCA
MscI 2004 TGGCCA TGG^CCA
4295 TGGCCA
NarI 175 GGCGCC GG^CGCC
2125 GGCGCC
NspI 4600 GCATGC [GA]CATG^[CT]
4838 GCATGC
PmaCI 2300 CACGTG CAC^GTG
4934 CACGTG
PmlI 2300 CACGTG CAC^GTG
4934 CACGTG
ScaI 3216 AGTACT AGT^ACT
4964 AGTACT
SexAI 339 ACCTGGT A^CC[AT]GGT
837 ACCTGGT
SfoI 175 GGCGCC GGC^GCC
2125 GGCGCC
SphI 4600 GCATGC GCATG^C
4838 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AflII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AspI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BfrI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI