################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AclI AgeI Alw44I AocI ApaLI AscI AseI AsnI AviII AvrII BbeI BclI BglII BlnI BsiEI BspDI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DrdI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KspI MfeI MluI MunI NarI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfoI SfuI SgrAI SmaI SphI Sse8387I SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 3345 GGTACC G^GTACC AccII 4201 CGCG CG^CG AccIII 2005 TCCGGA T^CCGGA AflII 378 CTTAAG C^TTAAG AhdI 5273 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 511 AGCGCT AGC^GCT Asp718I 3345 GGTACC G^GTACC AspEI 5273 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1718 CCCGAG C^[CT]CG[GA]G BamHI 5212 GGATCC G^GATCC BbsI 4315 GTCTTC GAAGAC(2/6) BbvCI 633 GCTGAGG CCTCAGC(-5/-2) BfrI 378 CTTAAG C^TTAAG BglI 3248 GCCGAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 4315 GTCTTC GAAGAC(2/6) BseAI 2005 TCCGGA T^CCGGA BsiWI 1299 CGTACG C^GTACG BsoBI 1718 CCCGAG C^[CT]CG[GA]G BspEI 2005 TCCGGA T^CCGGA BstUI 4201 CGCG CG^CG Eam1105I 5273 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 511 AGCGCT AGC^GCT HincII 3764 GTTGAC GT[CT]^[GA]AC HindII 3764 GTTGAC GT[CT]^[GA]AC KpnI 3345 GGTACC GGTAC^C MroI 2005 TCCGGA T^CCGGA MvnI 4201 CGCG CG^CG NaeI 4181 GCCGGC GCC^GGC NgoMIV 4181 GCCGGC G^CCGGC PflMI 4108 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ScaI 3840 AGTACT AGT^ACT SexAI 3302 ACCTGGT A^CC[AT]GGT SfiI 3247 GGCCGAGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SnaBI 3032 TACGTA TAC^GTA SpeI 151 ACTAGT A^CTAGT Van91I 4108 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1527 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 106 ACGTGT A^C[GA][CT]GT 5253 ACGTGT BalI 2635 TGGCCA TGG^CCA 3604 TGGCCA BcgI 3520 CGAAGGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4046 GCATTCCCCTCG BsaWI 2005 TCCGGA [AT]^CCGG[AT] 2754 TCCGGT BsmBI 2664 GAGACG CGTCTC(1/5) 4714 GAGACG BspHI 850 TCATGA T^CATGA 1535 TCATGA BsrDI 1369 GCAATG GCAATG(2/0) 4343 GCAATG BsrFI 1900 GCCGGT [GA]^CCGG[CT] 4181 GCCGGC BstAPI 2440 GCAAGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3387 GCATCTCCTGC Cfr10I 1900 GCCGGT [GA]^CCGG[CT] 4181 GCCGGC FauI 204 GCGGG CCCGC(4/6) 1995 GCGGG HaeII 511 AGCGCT [GA]GCGC^[CT] 4526 GGCGCT MluNI 2635 TGGCCA TGG^CCA 3604 TGGCCA MscI 2635 TGGCCA TGG^CCA 3604 TGGCCA NheI 588 GCTAGC G^CTAGC 4016 GCTAGC RcaI 850 TCATGA T^CATGA 1535 TCATGA SacI 27 GAGCTC GAGCT^C 1531 GAGCTC SapI 3749 GAAGAGC GCTCTTC(1/4) 4421 GAAGAGC SmlI 378 CTTAAG C^T[CT][GA]AG 4874 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AspI AvaII BanI BanII BbrPI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII XmnI