##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       AclI        AgeI        Alw44I      AocI        
ApaLI       AscI        AseI        AsnI        AviII       
AvrII       BbeI        BclI        BglII       BlnI        
BsiEI       BspDI       BspLU11I    BsrBI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      ClaI        CpoI        
DrdI        EagI        EclXI       Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KasI        
KspI        MfeI        MluI        MunI        NarI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SfoI        SfuI        SgrAI       
SmaI        SphI        Sse8387I    SspI        SwaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I       3345  GGTACC               G^GTACC
AccII        4201  CGCG                 CG^CG
AccIII       2005  TCCGGA               T^CCGGA
AflII         378  CTTAAG               C^TTAAG
AhdI         5273  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       511  AGCGCT               AGC^GCT
Asp718I      3345  GGTACC               G^GTACC
AspEI        5273  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1718  CCCGAG               C^[CT]CG[GA]G
BamHI        5212  GGATCC               G^GATCC
BbsI         4315  GTCTTC               GAAGAC(2/6)
BbvCI         633  GCTGAGG              CCTCAGC(-5/-2)
BfrI          378  CTTAAG               C^TTAAG
BglI         3248  GCCGAGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        4315  GTCTTC               GAAGAC(2/6)
BseAI        2005  TCCGGA               T^CCGGA
BsiWI        1299  CGTACG               C^GTACG
BsoBI        1718  CCCGAG               C^[CT]CG[GA]G
BspEI        2005  TCCGGA               T^CCGGA
BstUI        4201  CGCG                 CG^CG
Eam1105I     5273  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      511  AGCGCT               AGC^GCT
HincII       3764  GTTGAC               GT[CT]^[GA]AC
HindII       3764  GTTGAC               GT[CT]^[GA]AC
KpnI         3345  GGTACC               GGTAC^C
MroI         2005  TCCGGA               T^CCGGA
MvnI         4201  CGCG                 CG^CG
NaeI         4181  GCCGGC               GCC^GGC
NgoMIV       4181  GCCGGC               G^CCGGC
PflMI        4108  CCAAGATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI         3840  AGTACT               AGT^ACT
SexAI        3302  ACCTGGT              A^CC[AT]GGT
SfiI         3247  GGCCGAGTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI        3032  TACGTA               TAC^GTA
SpeI          151  ACTAGT               A^CTAGT
Van91I       4108  CCAAGATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1527  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AflIII        106  ACGTGT               A^C[GA][CT]GT
             5253  ACGTGT              
BalI         2635  TGGCCA               TGG^CCA
             3604  TGGCCA              
BcgI         3520  CGAAGGAGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4046  GCATTCCCCTCG        
BsaWI        2005  TCCGGA               [AT]^CCGG[AT]
             2754  TCCGGT              
BsmBI        2664  GAGACG               CGTCTC(1/5)
             4714  GAGACG              
BspHI         850  TCATGA               T^CATGA
             1535  TCATGA              
BsrDI        1369  GCAATG               GCAATG(2/0)
             4343  GCAATG              
BsrFI        1900  GCCGGT               [GA]^CCGG[CT]
             4181  GCCGGC              
BstAPI       2440  GCAAGGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3387  GCATCTCCTGC         
Cfr10I       1900  GCCGGT               [GA]^CCGG[CT]
             4181  GCCGGC              
FauI          204  GCGGG                CCCGC(4/6)
             1995  GCGGG               
HaeII         511  AGCGCT               [GA]GCGC^[CT]
             4526  GGCGCT              
MluNI        2635  TGGCCA               TGG^CCA
             3604  TGGCCA              
MscI         2635  TGGCCA               TGG^CCA
             3604  TGGCCA              
NheI          588  GCTAGC               G^CTAGC
             4016  GCTAGC              
RcaI          850  TCATGA               T^CATGA
             1535  TCATGA              
SacI           27  GAGCTC               GAGCT^C
             1531  GAGCTC              
SapI         3749  GAAGAGC              GCTCTTC(1/4)
             4421  GAAGAGC             
SmlI          378  CTTAAG               C^T[CT][GA]AG
             4874  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
Asp700I     AspHI       AspI        AvaII       BanI        
BanII       BbrPI       BbvI        BciVI       BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BspMI       BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DraIII      DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflFI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SspBI       
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     XcmI        XhoII       XmnI