##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AclI AgeI Alw44I AocI
ApaLI AscI AseI AsnI AviII
AvrII BbeI BclI BglII BlnI
BsiEI BspDI BspLU11I BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DrdI EagI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KspI MfeI MluI MunI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfoI SfuI SgrAI
SmaI SphI Sse8387I SspI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 3345 GGTACC G^GTACC
AccII 4201 CGCG CG^CG
AccIII 2005 TCCGGA T^CCGGA
AflII 378 CTTAAG C^TTAAG
AhdI 5273 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 511 AGCGCT AGC^GCT
Asp718I 3345 GGTACC G^GTACC
AspEI 5273 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1718 CCCGAG C^[CT]CG[GA]G
BamHI 5212 GGATCC G^GATCC
BbsI 4315 GTCTTC GAAGAC(2/6)
BbvCI 633 GCTGAGG CCTCAGC(-5/-2)
BfrI 378 CTTAAG C^TTAAG
BglI 3248 GCCGAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 4315 GTCTTC GAAGAC(2/6)
BseAI 2005 TCCGGA T^CCGGA
BsiWI 1299 CGTACG C^GTACG
BsoBI 1718 CCCGAG C^[CT]CG[GA]G
BspEI 2005 TCCGGA T^CCGGA
BstUI 4201 CGCG CG^CG
Eam1105I 5273 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 511 AGCGCT AGC^GCT
HincII 3764 GTTGAC GT[CT]^[GA]AC
HindII 3764 GTTGAC GT[CT]^[GA]AC
KpnI 3345 GGTACC GGTAC^C
MroI 2005 TCCGGA T^CCGGA
MvnI 4201 CGCG CG^CG
NaeI 4181 GCCGGC GCC^GGC
NgoMIV 4181 GCCGGC G^CCGGC
PflMI 4108 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 3840 AGTACT AGT^ACT
SexAI 3302 ACCTGGT A^CC[AT]GGT
SfiI 3247 GGCCGAGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 3032 TACGTA TAC^GTA
SpeI 151 ACTAGT A^CTAGT
Van91I 4108 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1527 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 106 ACGTGT A^C[GA][CT]GT
5253 ACGTGT
BalI 2635 TGGCCA TGG^CCA
3604 TGGCCA
BcgI 3520 CGAAGGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4046 GCATTCCCCTCG
BsaWI 2005 TCCGGA [AT]^CCGG[AT]
2754 TCCGGT
BsmBI 2664 GAGACG CGTCTC(1/5)
4714 GAGACG
BspHI 850 TCATGA T^CATGA
1535 TCATGA
BsrDI 1369 GCAATG GCAATG(2/0)
4343 GCAATG
BsrFI 1900 GCCGGT [GA]^CCGG[CT]
4181 GCCGGC
BstAPI 2440 GCAAGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3387 GCATCTCCTGC
Cfr10I 1900 GCCGGT [GA]^CCGG[CT]
4181 GCCGGC
FauI 204 GCGGG CCCGC(4/6)
1995 GCGGG
HaeII 511 AGCGCT [GA]GCGC^[CT]
4526 GGCGCT
MluNI 2635 TGGCCA TGG^CCA
3604 TGGCCA
MscI 2635 TGGCCA TGG^CCA
3604 TGGCCA
NheI 588 GCTAGC G^CTAGC
4016 GCTAGC
RcaI 850 TCATGA T^CATGA
1535 TCATGA
SacI 27 GAGCTC GAGCT^C
1531 GAGCTC
SapI 3749 GAAGAGC GCTCTTC(1/4)
4421 GAAGAGC
SmlI 378 CTTAAG C^T[CT][GA]AG
4874 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AspI AvaII BanI
BanII BbrPI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII XmnI