################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AccI AclI AgeI Aor51HI AscI AspI AviII BbrPI BciVI BsaAI BsaBI BsiWI BsmI BspDI BspHI BspLU11I Bst1107I BstBI BstZ17I ClaI CpoI DrdI Eco47III EcoRI EcoRV FseI FspI HincII HindII HpaI MamI MfeI MunI NcoI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccIII 1081 TCCGGA T^CCGGA AcsI 4492 AAATTT [GA]^AATT[CT] AflII 3727 CTTAAG C^TTAAG ApoI 4492 AAATTT [GA]^AATT[CT] AseI 4461 ATTAAT AT^TAAT AsnI 4461 ATTAAT AT^TAAT Asp700I 3747 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 388 CCTAGG C^CTAGG BbvCI 967 CCTCAGC CCTCAGC(-5/-2) BcgI 2529 GCATGAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 3727 CTTAAG C^TTAAG BglII 4435 AGATCT A^GATCT BlnI 388 CCTAGG C^CTAGG BsaWI 1081 TCCGGA [AT]^CCGG[AT] BseAI 1081 TCCGGA T^CCGGA BsgI 3569 GTGCAG GTGCAG(16/14) BsmBI 2095 GAGACG CGTCTC(1/5) Bsp1407I 2724 TGTACA T^GTACA BspEI 1081 TCCGGA T^CCGGA BsrGI 2724 TGTACA T^GTACA BssSI 321 CACGAG CACGAG(-5/-1) DraI 3950 TTTAAA TTT^AAA DraIII 3625 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 2625 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 3635 ATGCAT ATGCA^T KspI 343 CCGCGG CCGC^GG MluI 2515 ACGCGT A^CGCGT MroI 1081 TCCGGA T^CCGGA NsiI 3635 ATGCAT ATGCA^T PaeR7I 1598 CTCGAG C^TCGAG PshBI 4461 ATTAAT AT^TAAT SacII 343 CCGCGG CCGC^GG SspBI 2724 TGTACA T^GTACA SspI 4479 AATATT AAT^ATT XhoI 1598 CTCGAG C^TCGAG XmnI 3747 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 1375 GGTACC G^GTACC 2082 GGTACC AflIII 981 ACACGT A^C[GA][CT]GT 2515 ACGCGT AhdI 950 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4174 GACACAGAGTC Alw44I 773 GTGCAC G^TGCAC 3501 GTGCAC ApaLI 773 GTGCAC G^TGCAC 3501 GTGCAC Asp718I 1375 GGTACC G^GTACC 2082 GGTACC AspEI 950 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4174 GACACAGAGTC BalI 2521 TGGCCA TGG^CCA 2857 TGGCCA BamHI 688 GGATCC G^GATCC 1326 GGATCC BbsI 2489 GAAGAC GAAGAC(2/6) 3092 GTCTTC BclI 51 TGATCA T^GATCA 1857 TGATCA BpuAI 2489 GAAGAC GAAGAC(2/6) 3092 GTCTTC BsiEI 143 CGGCCG CG[GA][CT]^CG 2077 CGGCCG BstEII 1657 GGTGACC G^GT[ACGT]ACC 3339 GGTAACC BstPI 1657 GGTGACC G^GT[ACGT]ACC 3339 GGTAACC EagI 143 CGGCCG C^GGCCG 2077 CGGCCG Eam1105I 950 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4174 GACACAGAGTC EclXI 143 CGGCCG C^GGCCG 2077 CGGCCG Eco52I 143 CGGCCG C^GGCCG 2077 CGGCCG EcoO65I 1657 GGTGACC G^GT[ACGT]ACC 3339 GGTAACC FbaI 51 TGATCA T^GATCA 1857 TGATCA HindIII 313 AAGCTT A^AGCTT 3618 AAGCTT KpnI 1375 GGTACC GGTAC^C 2082 GGTACC MluNI 2521 TGGCCA TGG^CCA 2857 TGGCCA MscI 2521 TGGCCA TGG^CCA 2857 TGGCCA SapI 929 GAAGAGC GCTCTTC(1/4) 1984 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI