##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccI AclI AgeI Aor51HI
AscI AspI AviII BbrPI BciVI
BsaAI BsaBI BsiWI BsmI BspDI
BspHI BspLU11I Bst1107I BstBI BstZ17I
ClaI CpoI DrdI Eco47III EcoRI
EcoRV FseI FspI HincII HindII
HpaI MamI MfeI MunI NcoI
NdeI NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 1081 TCCGGA T^CCGGA
AcsI 4492 AAATTT [GA]^AATT[CT]
AflII 3727 CTTAAG C^TTAAG
ApoI 4492 AAATTT [GA]^AATT[CT]
AseI 4461 ATTAAT AT^TAAT
AsnI 4461 ATTAAT AT^TAAT
Asp700I 3747 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 388 CCTAGG C^CTAGG
BbvCI 967 CCTCAGC CCTCAGC(-5/-2)
BcgI 2529 GCATGAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3727 CTTAAG C^TTAAG
BglII 4435 AGATCT A^GATCT
BlnI 388 CCTAGG C^CTAGG
BsaWI 1081 TCCGGA [AT]^CCGG[AT]
BseAI 1081 TCCGGA T^CCGGA
BsgI 3569 GTGCAG GTGCAG(16/14)
BsmBI 2095 GAGACG CGTCTC(1/5)
Bsp1407I 2724 TGTACA T^GTACA
BspEI 1081 TCCGGA T^CCGGA
BsrGI 2724 TGTACA T^GTACA
BssSI 321 CACGAG CACGAG(-5/-1)
DraI 3950 TTTAAA TTT^AAA
DraIII 3625 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2625 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3635 ATGCAT ATGCA^T
KspI 343 CCGCGG CCGC^GG
MluI 2515 ACGCGT A^CGCGT
MroI 1081 TCCGGA T^CCGGA
NsiI 3635 ATGCAT ATGCA^T
PaeR7I 1598 CTCGAG C^TCGAG
PshBI 4461 ATTAAT AT^TAAT
SacII 343 CCGCGG CCGC^GG
SspBI 2724 TGTACA T^GTACA
SspI 4479 AATATT AAT^ATT
XhoI 1598 CTCGAG C^TCGAG
XmnI 3747 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 1375 GGTACC G^GTACC
2082 GGTACC
AflIII 981 ACACGT A^C[GA][CT]GT
2515 ACGCGT
AhdI 950 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4174 GACACAGAGTC
Alw44I 773 GTGCAC G^TGCAC
3501 GTGCAC
ApaLI 773 GTGCAC G^TGCAC
3501 GTGCAC
Asp718I 1375 GGTACC G^GTACC
2082 GGTACC
AspEI 950 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4174 GACACAGAGTC
BalI 2521 TGGCCA TGG^CCA
2857 TGGCCA
BamHI 688 GGATCC G^GATCC
1326 GGATCC
BbsI 2489 GAAGAC GAAGAC(2/6)
3092 GTCTTC
BclI 51 TGATCA T^GATCA
1857 TGATCA
BpuAI 2489 GAAGAC GAAGAC(2/6)
3092 GTCTTC
BsiEI 143 CGGCCG CG[GA][CT]^CG
2077 CGGCCG
BstEII 1657 GGTGACC G^GT[ACGT]ACC
3339 GGTAACC
BstPI 1657 GGTGACC G^GT[ACGT]ACC
3339 GGTAACC
EagI 143 CGGCCG C^GGCCG
2077 CGGCCG
Eam1105I 950 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4174 GACACAGAGTC
EclXI 143 CGGCCG C^GGCCG
2077 CGGCCG
Eco52I 143 CGGCCG C^GGCCG
2077 CGGCCG
EcoO65I 1657 GGTGACC G^GT[ACGT]ACC
3339 GGTAACC
FbaI 51 TGATCA T^GATCA
1857 TGATCA
HindIII 313 AAGCTT A^AGCTT
3618 AAGCTT
KpnI 1375 GGTACC GGTAC^C
2082 GGTACC
MluNI 2521 TGGCCA TGG^CCA
2857 TGGCCA
MscI 2521 TGGCCA TGG^CCA
2857 TGGCCA
SapI 929 GAAGAGC GCTCTTC(1/4)
1984 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI