##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccIII AflIII AgeI Aor51HI
ApaI AscI AseI AsnI AspI
AviII AvrII BalI BamHI BbrPI
BcgI BciVI BclI BglI BglII
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraIII Eco47III EcoO65I EcoRV
FbaI FseI FspI KspI MamI
MluI MluNI MroI MscI NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SbfI SfiI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1537 GGTACC G^GTACC
AccII 193 CGCG CG^CG
AclI 2477 AACGTT AA^CGTT
AhdI 2015 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1081 CCTGAGG CC^T[ACGT]AGG
Asp700I 347 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1537 GGTACC G^GTACC
AspEI 2015 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 182 CTCGAG C^[CT]CG[GA]G
BbeI 107 GGCGCC GGCGC^C
BbsI 1073 GAAGAC GAAGAC(2/6)
BpuAI 1073 GAAGAC GAAGAC(2/6)
BsmI 1102 GAATGC GAATGC(1/-1)
BsoBI 182 CTCGAG C^[CT]CG[GA]G
BspHI 4553 TCATGA T^CATGA
BstUI 193 CGCG CG^CG
Bsu36I 1081 CCTGAGG CC^T[ACGT]AGG
DrdI 4407 GACTAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2015 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1081 CCTGAGG CC^T[ACGT]AGG
EcoNI 3097 CCTGAGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 648 GAATTC G^AATTC
EcoT22I 2837 ATGCAT ATGCA^T
HaeII 107 GGCGCC [GA]GCGC^[CT]
HpaI 2640 GTTAAC GTT^AAC
KasI 107 GGCGCC G^GCGCC
KpnI 1537 GGTACC GGTAC^C
MvnI 193 CGCG CG^CG
NarI 107 GGCGCC GG^CGCC
NsiI 2837 ATGCAT ATGCA^T
PaeR7I 182 CTCGAG C^TCGAG
Psp1406I 2477 AACGTT AA^CGTT
RcaI 4553 TCATGA T^CATGA
SapI 1730 GAAGAGC GCTCTTC(1/4)
ScaI 794 AGTACT AGT^ACT
SfoI 107 GGCGCC GGC^GCC
SpeI 3997 ACTAGT A^CTAGT
XhoI 182 CTCGAG C^TCGAG
XmnI 347 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3147 GTAGAC GT^[AC][GT]AC
4674 GTAGAC
AcyI 107 GGCGCC G[GA]^CG[CT]C
1076 GACGCC
AflII 3433 CTTAAG C^TTAAG
3485 CTTAAG
Alw44I 3215 GTGCAC G^TGCAC
4138 GTGCAC
ApaLI 3215 GTGCAC G^TGCAC
4138 GTGCAC
BanII 1041 GAGCTC G[GA]GC[CT]^C
1628 GAGCTC
BbvCI 971 GCTGAGG CCTCAGC(-5/-2)
2658 GCTGAGG
BcnI 136 CCCGG CC^[GC]GG
1584 CCGGG
BfrI 3433 CTTAAG C^TTAAG
3485 CTTAAG
BsaHI 107 GGCGCC G[GA]^CG[CT]C
1076 GACGCC
BsiEI 171 CGGCCG CG[GA][CT]^CG
201 CGGCCG
BspMI 3603 ACCTGC ACCTGC(4/8)
4560 ACCTGC
BsrFI 199 GCCGGC [GA]^CCGG[CT]
1735 GCCGGC
BstAPI 2489 GCATTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3943 GCAGTCACTGC
BstYI 1115 GGATCT [GA]^GATC[CT]
3972 GGATCT
Cfr10I 199 GCCGGC [GA]^CCGG[CT]
1735 GCCGGC
DraII 1903 GGGTCCT [GA]G^G[ACGT]CC[CT]
3405 AGGACCT
EagI 171 CGGCCG C^GGCCG
201 CGGCCG
EclXI 171 CGGCCG C^GGCCG
201 CGGCCG
Eco52I 171 CGGCCG C^GGCCG
201 CGGCCG
EcoO109I 1903 GGGTCCT [GA]G^G[ACGT]CC[CT]
3405 AGGACCT
Hin1I 107 GGCGCC G[GA]^CG[CT]C
1076 GACGCC
HincII 2640 GTTAAC GT[CT]^[GA]AC
4640 GTTGAC
HindII 2640 GTTAAC GT[CT]^[GA]AC
4640 GTTGAC
MfeI 3663 CAATTG C^AATTG
4901 CAATTG
MflI 1115 GGATCT [GA]^GATC[CT]
3972 GGATCT
MunI 3663 CAATTG C^AATTG
4901 CAATTG
NaeI 199 GCCGGC GCC^GGC
1735 GCCGGC
NciI 136 CCCGG CC^[GC]GG
1584 CCGGG
NdeI 1830 CATATG CA^TATG
3423 CATATG
NgoMIV 199 GCCGGC G^CCGGC
1735 GCCGGC
PflMI 3194 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4076 CCAACTCCTGG
PpuMI 1903 GGGTCCT [GA]G^G[AT]CC[CT]
3405 AGGACCT
SacI 1041 GAGCTC GAGCT^C
1628 GAGCTC
SexAI 3018 ACCAGGT A^CC[AT]GGT
3151 ACCAGGT
Van91I 3194 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4076 CCAACTCCTGG
XhoII 1115 GGATCT [GA]^GATC[CT]
3972 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BstF5I BstNI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI