################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII AccIII AflIII AgeI Aor51HI ApaI AscI AseI AsnI AspI AviII AvrII BalI BamHI BbrPI BcgI BciVI BclI BglI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraIII Eco47III EcoO65I EcoRV FbaI FseI FspI KspI MamI MluI MluNI MroI MscI NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 1537 GGTACC G^GTACC AccII 193 CGCG CG^CG AclI 2477 AACGTT AA^CGTT AhdI 2015 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1081 CCTGAGG CC^T[ACGT]AGG Asp700I 347 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1537 GGTACC G^GTACC AspEI 2015 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 182 CTCGAG C^[CT]CG[GA]G BbeI 107 GGCGCC GGCGC^C BbsI 1073 GAAGAC GAAGAC(2/6) BpuAI 1073 GAAGAC GAAGAC(2/6) BsmI 1102 GAATGC GAATGC(1/-1) BsoBI 182 CTCGAG C^[CT]CG[GA]G BspHI 4553 TCATGA T^CATGA BstUI 193 CGCG CG^CG Bsu36I 1081 CCTGAGG CC^T[ACGT]AGG DrdI 4407 GACTAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2015 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1081 CCTGAGG CC^T[ACGT]AGG EcoNI 3097 CCTGAGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 648 GAATTC G^AATTC EcoT22I 2837 ATGCAT ATGCA^T HaeII 107 GGCGCC [GA]GCGC^[CT] HpaI 2640 GTTAAC GTT^AAC KasI 107 GGCGCC G^GCGCC KpnI 1537 GGTACC GGTAC^C MvnI 193 CGCG CG^CG NarI 107 GGCGCC GG^CGCC NsiI 2837 ATGCAT ATGCA^T PaeR7I 182 CTCGAG C^TCGAG Psp1406I 2477 AACGTT AA^CGTT RcaI 4553 TCATGA T^CATGA SapI 1730 GAAGAGC GCTCTTC(1/4) ScaI 794 AGTACT AGT^ACT SfoI 107 GGCGCC GGC^GCC SpeI 3997 ACTAGT A^CTAGT XhoI 182 CTCGAG C^TCGAG XmnI 347 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccI 3147 GTAGAC GT^[AC][GT]AC 4674 GTAGAC AcyI 107 GGCGCC G[GA]^CG[CT]C 1076 GACGCC AflII 3433 CTTAAG C^TTAAG 3485 CTTAAG Alw44I 3215 GTGCAC G^TGCAC 4138 GTGCAC ApaLI 3215 GTGCAC G^TGCAC 4138 GTGCAC BanII 1041 GAGCTC G[GA]GC[CT]^C 1628 GAGCTC BbvCI 971 GCTGAGG CCTCAGC(-5/-2) 2658 GCTGAGG BcnI 136 CCCGG CC^[GC]GG 1584 CCGGG BfrI 3433 CTTAAG C^TTAAG 3485 CTTAAG BsaHI 107 GGCGCC G[GA]^CG[CT]C 1076 GACGCC BsiEI 171 CGGCCG CG[GA][CT]^CG 201 CGGCCG BspMI 3603 ACCTGC ACCTGC(4/8) 4560 ACCTGC BsrFI 199 GCCGGC [GA]^CCGG[CT] 1735 GCCGGC BstAPI 2489 GCATTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3943 GCAGTCACTGC BstYI 1115 GGATCT [GA]^GATC[CT] 3972 GGATCT Cfr10I 199 GCCGGC [GA]^CCGG[CT] 1735 GCCGGC DraII 1903 GGGTCCT [GA]G^G[ACGT]CC[CT] 3405 AGGACCT EagI 171 CGGCCG C^GGCCG 201 CGGCCG EclXI 171 CGGCCG C^GGCCG 201 CGGCCG Eco52I 171 CGGCCG C^GGCCG 201 CGGCCG EcoO109I 1903 GGGTCCT [GA]G^G[ACGT]CC[CT] 3405 AGGACCT Hin1I 107 GGCGCC G[GA]^CG[CT]C 1076 GACGCC HincII 2640 GTTAAC GT[CT]^[GA]AC 4640 GTTGAC HindII 2640 GTTAAC GT[CT]^[GA]AC 4640 GTTGAC MfeI 3663 CAATTG C^AATTG 4901 CAATTG MflI 1115 GGATCT [GA]^GATC[CT] 3972 GGATCT MunI 3663 CAATTG C^AATTG 4901 CAATTG NaeI 199 GCCGGC GCC^GGC 1735 GCCGGC NciI 136 CCCGG CC^[GC]GG 1584 CCGGG NdeI 1830 CATATG CA^TATG 3423 CATATG NgoMIV 199 GCCGGC G^CCGGC 1735 GCCGGC PflMI 3194 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4076 CCAACTCCTGG PpuMI 1903 GGGTCCT [GA]G^G[AT]CC[CT] 3405 AGGACCT SacI 1041 GAGCTC GAGCT^C 1628 GAGCTC SexAI 3018 ACCAGGT A^CC[AT]GGT 3151 ACCAGGT Van91I 3194 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4076 CCAACTCCTGG XhoII 1115 GGATCT [GA]^GATC[CT] 3972 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI