##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AcyI AgeI Aor51HI
AscI Asp718I AspI AviII AvrII
BalI BbeI BcgI BciVI BclI
BglI BlnI BsaBI BsaHI BsgI
BsiEI BsiWI BsmBI BspDI BspHI
BspLU11I BsrFI BstBI Cfr10I ClaI
CpoI EagI EclXI Eco47III Eco52I
FbaI FseI FspI HaeII Hin1I
HpaI KasI KpnI KspI MamI
MluI MluNI MscI NaeI NarI
NgoMIV NotI NruI NspV PflFI
PinAI PmeI PshAI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 55 AACGTT AA^CGTT
AflII 4106 CTTAAG C^TTAAG
AflIII 3816 ACACGT A^C[GA][CT]GT
AhdI 1217 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2362 CCTCAGG CC^T[ACGT]AGG
AseI 1569 ATTAAT AT^TAAT
AsnI 1569 ATTAAT AT^TAAT
AspEI 1217 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2667 GGATCC G^GATCC
BbrPI 3817 CACGTG CAC^GTG
BbvCI 178 GCTGAGG CCTCAGC(-5/-2)
BfrI 4106 CTTAAG C^TTAAG
BglII 5736 AGATCT A^GATCT
BsaAI 3817 CACGTG [CT]AC^GT[GA]
Bsp1407I 4520 TGTACA T^GTACA
BsrBI 3440 CCGCTC CCGCTC(-3/-3)
BsrGI 4520 TGTACA T^GTACA
BssHII 4953 GCGCGC G^CGCGC
BssSI 1754 CACGAG CACGAG(-5/-1)
Bst1107I 877 GTATAC GTA^TAC
BstXI 1899 CCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 877 GTATAC GTA^TAC
Bsu36I 2362 CCTCAGG CC^T[ACGT]AGG
DraIII 4698 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2582 TGGCCG [CT]^GGCC[GA]
Eam1105I 1217 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2362 CCTCAGG CC^T[ACGT]AGG
EcoRI 4002 GAATTC G^AATTC
EcoRV 2930 GATATC GAT^ATC
EcoT22I 3424 ATGCAT ATGCA^T
HincII 1442 GTCAAC GT[CT]^[GA]AC
HindII 1442 GTCAAC GT[CT]^[GA]AC
HindIII 1776 AAGCTT A^AGCTT
NsiI 3424 ATGCAT ATGCA^T
PacI 5767 TTAATTAA TTAAT^TAA
PmaCI 3817 CACGTG CAC^GTG
PmlI 3817 CACGTG CAC^GTG
PshBI 1569 ATTAAT AT^TAAT
Psp1406I 55 AACGTT AA^CGTT
SexAI 2069 ACCAGGT A^CC[AT]GGT
SmaI 3723 CCCGGG CCC^GGG
SphI 627 GCATGC GCATG^C
SspBI 4520 TGTACA T^GTACA
XbaI 2484 TCTAGA T^CTAGA
XmaCI 3723 CCCGGG C^CCGGG
XmaI 3723 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 2721 TCCGGA T^CCGGA
3733 TCCGGA
Alw44I 3666 GTGCAC G^TGCAC
4704 GTGCAC
ApaI 3433 GGGCCC GGGCC^C
4565 GGGCCC
ApaLI 3666 GTGCAC G^TGCAC
4704 GTGCAC
BanI 3337 GGCACC G^G[CT][GA]CC
3493 GGTGCC
BsaWI 2721 TCCGGA [AT]^CCGG[AT]
3733 TCCGGA
BseAI 2721 TCCGGA T^CCGGA
3733 TCCGGA
BsmI 5422 GCATTC GAATGC(1/-1)
5439 GCATTC
BspEI 2721 TCCGGA T^CCGGA
3733 TCCGGA
BspMI 2229 GCAGGT ACCTGC(4/8)
3597 GCAGGT
BstEII 1728 GGTCACC G^GT[ACGT]ACC
2639 GGTCACC
BstPI 1728 GGTCACC G^GT[ACGT]ACC
2639 GGTCACC
DraI 1735 TTTAAA TTT^AAA
2842 TTTAAA
DrdI 1821 GACTCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3051 GACCCCATGGTC
EcoNI 617 CCTACTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4066 CCTGTGAGAGG
EcoO65I 1728 GGTCACC G^GT[ACGT]ACC
2639 GGTCACC
HgaI 498 GACGC GACGC(5/10)
4611 GCGTC
MfeI 2224 CAATTG C^AATTG
4902 CAATTG
MroI 2721 TCCGGA T^CCGGA
3733 TCCGGA
MunI 2224 CAATTG C^AATTG
4902 CAATTG
NdeI 1955 CATATG CA^TATG
4483 CATATG
NheI 391 GCTAGC G^CTAGC
3322 GCTAGC
PaeR7I 4221 CTCGAG C^TCGAG
4663 CTCGAG
SacI 2703 GAGCTC GAGCT^C
2869 GAGCTC
SapI 2761 GAAGAGC GCTCTTC(1/4)
5130 GCTCTTC
SspI 121 AATATT AAT^ATT
4863 AATATT
XhoI 4221 CTCGAG C^TCGAG
4663 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI