################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII Acc65I AcyI AgeI Aor51HI AscI Asp718I AspI AviII AvrII BalI BbeI BcgI BciVI BclI BglI BlnI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI BspDI BspHI BspLU11I BsrFI BstBI Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I FbaI FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MamI MluI MluNI MscI NaeI NarI NgoMIV NotI NruI NspV PflFI PinAI PmeI PshAI PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI Tth111I ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AclI 55 AACGTT AA^CGTT AflII 4106 CTTAAG C^TTAAG AflIII 3816 ACACGT A^C[GA][CT]GT AhdI 1217 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2362 CCTCAGG CC^T[ACGT]AGG AseI 1569 ATTAAT AT^TAAT AsnI 1569 ATTAAT AT^TAAT AspEI 1217 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 2667 GGATCC G^GATCC BbrPI 3817 CACGTG CAC^GTG BbvCI 178 GCTGAGG CCTCAGC(-5/-2) BfrI 4106 CTTAAG C^TTAAG BglII 5736 AGATCT A^GATCT BsaAI 3817 CACGTG [CT]AC^GT[GA] Bsp1407I 4520 TGTACA T^GTACA BsrBI 3440 CCGCTC CCGCTC(-3/-3) BsrGI 4520 TGTACA T^GTACA BssHII 4953 GCGCGC G^CGCGC BssSI 1754 CACGAG CACGAG(-5/-1) Bst1107I 877 GTATAC GTA^TAC BstXI 1899 CCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 877 GTATAC GTA^TAC Bsu36I 2362 CCTCAGG CC^T[ACGT]AGG DraIII 4698 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2582 TGGCCG [CT]^GGCC[GA] Eam1105I 1217 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2362 CCTCAGG CC^T[ACGT]AGG EcoRI 4002 GAATTC G^AATTC EcoRV 2930 GATATC GAT^ATC EcoT22I 3424 ATGCAT ATGCA^T HincII 1442 GTCAAC GT[CT]^[GA]AC HindII 1442 GTCAAC GT[CT]^[GA]AC HindIII 1776 AAGCTT A^AGCTT NsiI 3424 ATGCAT ATGCA^T PacI 5767 TTAATTAA TTAAT^TAA PmaCI 3817 CACGTG CAC^GTG PmlI 3817 CACGTG CAC^GTG PshBI 1569 ATTAAT AT^TAAT Psp1406I 55 AACGTT AA^CGTT SexAI 2069 ACCAGGT A^CC[AT]GGT SmaI 3723 CCCGGG CCC^GGG SphI 627 GCATGC GCATG^C SspBI 4520 TGTACA T^GTACA XbaI 2484 TCTAGA T^CTAGA XmaCI 3723 CCCGGG C^CCGGG XmaI 3723 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccIII 2721 TCCGGA T^CCGGA 3733 TCCGGA Alw44I 3666 GTGCAC G^TGCAC 4704 GTGCAC ApaI 3433 GGGCCC GGGCC^C 4565 GGGCCC ApaLI 3666 GTGCAC G^TGCAC 4704 GTGCAC BanI 3337 GGCACC G^G[CT][GA]CC 3493 GGTGCC BsaWI 2721 TCCGGA [AT]^CCGG[AT] 3733 TCCGGA BseAI 2721 TCCGGA T^CCGGA 3733 TCCGGA BsmI 5422 GCATTC GAATGC(1/-1) 5439 GCATTC BspEI 2721 TCCGGA T^CCGGA 3733 TCCGGA BspMI 2229 GCAGGT ACCTGC(4/8) 3597 GCAGGT BstEII 1728 GGTCACC G^GT[ACGT]ACC 2639 GGTCACC BstPI 1728 GGTCACC G^GT[ACGT]ACC 2639 GGTCACC DraI 1735 TTTAAA TTT^AAA 2842 TTTAAA DrdI 1821 GACTCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3051 GACCCCATGGTC EcoNI 617 CCTACTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4066 CCTGTGAGAGG EcoO65I 1728 GGTCACC G^GT[ACGT]ACC 2639 GGTCACC HgaI 498 GACGC GACGC(5/10) 4611 GCGTC MfeI 2224 CAATTG C^AATTG 4902 CAATTG MroI 2721 TCCGGA T^CCGGA 3733 TCCGGA MunI 2224 CAATTG C^AATTG 4902 CAATTG NdeI 1955 CATATG CA^TATG 4483 CATATG NheI 391 GCTAGC G^CTAGC 3322 GCTAGC PaeR7I 4221 CTCGAG C^TCGAG 4663 CTCGAG SacI 2703 GAGCTC GAGCT^C 2869 GAGCTC SapI 2761 GAAGAGC GCTCTTC(1/4) 5130 GCTCTTC SspI 121 AATATT AAT^ATT 4863 AATATT XhoI 4221 CTCGAG C^TCGAG 4663 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI