################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AccIII AclI AcsI AgeI AhdI ApoI AscI AseI AsnI AspEI AviII BcgI BglII BsaBI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FseI FspI HgaI HpaI MamI MfeI MluI MroI MunI NdeI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Alw44I 3702 GTGCAC G^TGCAC Aor51HI 1280 AGCGCT AGC^GCT ApaLI 3702 GTGCAC G^TGCAC Asp700I 1278 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 12 CCTAGG C^CTAGG BamHI 3146 GGATCC G^GATCC BbeI 1827 GGCGCC GGCGC^C BbrPI 2915 CACGTG CAC^GTG BciVI 4532 GTATCC GTATCC(6/5) BclI 2513 TGATCA T^GATCA BglI 2701 GCCACGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 12 CCTAGG C^CTAGG BsiEI 4556 CGGCCG CG[GA][CT]^CG BspLU11I 1784 ACATGT A^CATGT BsrBI 4210 GAGCGG CCGCTC(-3/-3) BstAPI 159 GCACAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1390 GGTGACC G^GT[ACGT]ACC BstPI 1390 GGTGACC G^GT[ACGT]ACC DrdI 1815 GACGTGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 4556 CGGCCG C^GGCCG EclXI 4556 CGGCCG C^GGCCG Eco47III 1280 AGCGCT AGC^GCT Eco52I 4556 CGGCCG C^GGCCG EcoO65I 1390 GGTGACC G^GT[ACGT]ACC FbaI 2513 TGATCA T^GATCA HincII 2758 GTCAAC GT[CT]^[GA]AC HindII 2758 GTCAAC GT[CT]^[GA]AC KasI 1827 GGCGCC G^GCGCC KspI 2567 CCGCGG CCGC^GG NaeI 28 GCCGGC GCC^GGC NarI 1827 GGCGCC GG^CGCC NgoMIV 28 GCCGGC G^CCGGC NheI 2284 GCTAGC G^CTAGC NotI 4555 GCGGCCGC GC^GGCCGC PaeR7I 1231 CTCGAG C^TCGAG PmaCI 2915 CACGTG CAC^GTG PmlI 2915 CACGTG CAC^GTG PshAI 1129 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 2567 CCGCGG CCGC^GG SexAI 2489 ACCTGGT A^CC[AT]GGT SfoI 1827 GGCGCC GGC^GCC SmaI 2084 CCCGGG CCC^GGG SphI 2162 GCATGC GCATG^C XhoI 1231 CTCGAG C^TCGAG XmaCI 2084 CCCGGG C^CCGGG XmaI 2084 CCCGGG C^CCGGG XmnI 1278 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AatII 1821 GACGTC GACGT^C 3669 GACGTC Acc65I 590 GGTACC G^GTACC 3554 GGTACC AccII 2568 CGCG CG^CG 3404 CGCG AflII 1617 CTTAAG C^TTAAG 3763 CTTAAG AocI 662 CCTCAGG CC^T[ACGT]AGG 3601 CCTAAGG ApaI 3452 GGGCCC GGGCC^C 4214 GGGCCC Asp718I 590 GGTACC G^GTACC 3554 GGTACC AvaI 1231 CTCGAG C^[CT]CG[GA]G 2084 CCCGGG BbvCI 4724 CCTCAGC CCTCAGC(-5/-2) 4110 GCTGAGG BfrI 1617 CTTAAG C^TTAAG 3763 CTTAAG BsaI 3 GGTCTC GGTCTC(1/5) 744 GGTCTC BsmBI 250 GAGACG CGTCTC(1/5) 1813 GAGACG BsoBI 1231 CTCGAG C^[CT]CG[GA]G 2084 CCCGGG Bsp1407I 3122 TGTACA T^GTACA 3398 TGTACA BsrGI 3122 TGTACA T^GTACA 3398 TGTACA BssSI 753 CACGAG CACGAG(-5/-1) 3380 CTCGTG BstUI 2568 CGCG CG^CG 3404 CGCG Bsu36I 662 CCTCAGG CC^T[ACGT]AGG 3601 CCTAAGG DraIII 160 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG 3295 CACAGCGTG Eco81I 662 CCTCAGG CC^T[ACGT]AGG 3601 CCTAAGG HindIII 334 AAGCTT A^AGCTT 3526 AAGCTT KpnI 590 GGTACC GGTAC^C 3554 GGTACC MvnI 2568 CGCG CG^CG 3404 CGCG SapI 4207 GAAGAGC GCTCTTC(1/4) 1995 GCTCTTC SspBI 3122 TGTACA T^GTACA 3398 TGTACA StuI 3278 AGGCCT AGG^CCT 4188 AGGCCT Tsp509I 2485 AATT ^AATT 4396 AATT XbaI 367 TCTAGA T^CTAGA 3573 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AciI AcyI AfaI AflIII AluI AlwI AlwNI AspHI AspI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI Tth111I TthHB8I Van91I XcmI XhoII