##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccIII AclI AcsI AgeI AhdI
ApoI AscI AseI AsnI AspEI
AviII BcgI BglII BsaBI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspHI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eam1105I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HpaI MamI MfeI MluI MroI
MunI NdeI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Alw44I 3702 GTGCAC G^TGCAC
Aor51HI 1280 AGCGCT AGC^GCT
ApaLI 3702 GTGCAC G^TGCAC
Asp700I 1278 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 12 CCTAGG C^CTAGG
BamHI 3146 GGATCC G^GATCC
BbeI 1827 GGCGCC GGCGC^C
BbrPI 2915 CACGTG CAC^GTG
BciVI 4532 GTATCC GTATCC(6/5)
BclI 2513 TGATCA T^GATCA
BglI 2701 GCCACGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 12 CCTAGG C^CTAGG
BsiEI 4556 CGGCCG CG[GA][CT]^CG
BspLU11I 1784 ACATGT A^CATGT
BsrBI 4210 GAGCGG CCGCTC(-3/-3)
BstAPI 159 GCACAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1390 GGTGACC G^GT[ACGT]ACC
BstPI 1390 GGTGACC G^GT[ACGT]ACC
DrdI 1815 GACGTGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4556 CGGCCG C^GGCCG
EclXI 4556 CGGCCG C^GGCCG
Eco47III 1280 AGCGCT AGC^GCT
Eco52I 4556 CGGCCG C^GGCCG
EcoO65I 1390 GGTGACC G^GT[ACGT]ACC
FbaI 2513 TGATCA T^GATCA
HincII 2758 GTCAAC GT[CT]^[GA]AC
HindII 2758 GTCAAC GT[CT]^[GA]AC
KasI 1827 GGCGCC G^GCGCC
KspI 2567 CCGCGG CCGC^GG
NaeI 28 GCCGGC GCC^GGC
NarI 1827 GGCGCC GG^CGCC
NgoMIV 28 GCCGGC G^CCGGC
NheI 2284 GCTAGC G^CTAGC
NotI 4555 GCGGCCGC GC^GGCCGC
PaeR7I 1231 CTCGAG C^TCGAG
PmaCI 2915 CACGTG CAC^GTG
PmlI 2915 CACGTG CAC^GTG
PshAI 1129 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2567 CCGCGG CCGC^GG
SexAI 2489 ACCTGGT A^CC[AT]GGT
SfoI 1827 GGCGCC GGC^GCC
SmaI 2084 CCCGGG CCC^GGG
SphI 2162 GCATGC GCATG^C
XhoI 1231 CTCGAG C^TCGAG
XmaCI 2084 CCCGGG C^CCGGG
XmaI 2084 CCCGGG C^CCGGG
XmnI 1278 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 1821 GACGTC GACGT^C
3669 GACGTC
Acc65I 590 GGTACC G^GTACC
3554 GGTACC
AccII 2568 CGCG CG^CG
3404 CGCG
AflII 1617 CTTAAG C^TTAAG
3763 CTTAAG
AocI 662 CCTCAGG CC^T[ACGT]AGG
3601 CCTAAGG
ApaI 3452 GGGCCC GGGCC^C
4214 GGGCCC
Asp718I 590 GGTACC G^GTACC
3554 GGTACC
AvaI 1231 CTCGAG C^[CT]CG[GA]G
2084 CCCGGG
BbvCI 4724 CCTCAGC CCTCAGC(-5/-2)
4110 GCTGAGG
BfrI 1617 CTTAAG C^TTAAG
3763 CTTAAG
BsaI 3 GGTCTC GGTCTC(1/5)
744 GGTCTC
BsmBI 250 GAGACG CGTCTC(1/5)
1813 GAGACG
BsoBI 1231 CTCGAG C^[CT]CG[GA]G
2084 CCCGGG
Bsp1407I 3122 TGTACA T^GTACA
3398 TGTACA
BsrGI 3122 TGTACA T^GTACA
3398 TGTACA
BssSI 753 CACGAG CACGAG(-5/-1)
3380 CTCGTG
BstUI 2568 CGCG CG^CG
3404 CGCG
Bsu36I 662 CCTCAGG CC^T[ACGT]AGG
3601 CCTAAGG
DraIII 160 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
3295 CACAGCGTG
Eco81I 662 CCTCAGG CC^T[ACGT]AGG
3601 CCTAAGG
HindIII 334 AAGCTT A^AGCTT
3526 AAGCTT
KpnI 590 GGTACC GGTAC^C
3554 GGTACC
MvnI 2568 CGCG CG^CG
3404 CGCG
SapI 4207 GAAGAGC GCTCTTC(1/4)
1995 GCTCTTC
SspBI 3122 TGTACA T^GTACA
3398 TGTACA
StuI 3278 AGGCCT AGG^CCT
4188 AGGCCT
Tsp509I 2485 AATT ^AATT
4396 AATT
XbaI 367 TCTAGA T^CTAGA
3573 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AspI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XcmI XhoII