################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AccIII AgeI Aor51HI AscI AviII AvrII BalI BamHI BbeI BclI BcnI BglI BlnI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI BstBI Cfr10I ClaI CpoI EaeI EagI EclXI Eco47III Eco52I FbaI FseI FspI KasI KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NspV PacI PaeR7I PinAI PmeI PshAI PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1873 GTATAC GT^[AC][GT]AC AclI 1822 AACGTT AA^CGTT AcyI 994 GACGCC G[GA]^CG[CT]C AflIII 3895 ACACGT A^C[GA][CT]GT AhdI 84 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2235 GTGCAC G^TGCAC ApaI 3260 GGGCCC GGGCC^C ApaLI 2235 GTGCAC G^TGCAC AseI 4411 ATTAAT AT^TAAT AsnI 4411 ATTAAT AT^TAAT Asp700I 3368 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 84 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 4035 CTCGGG C^[CT]CG[GA]G BbrPI 2238 CACGTG CAC^GTG BbvCI 4073 GCTGAGG CCTCAGC(-5/-2) BcgI 223 CGAGTTATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 454 GCTCAGC GC^T[ACGT]AGC Bpu1102I 454 GCTCAGC GC^T[ACGT]AGC BsaBI 3218 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 994 GACGCC G[GA]^CG[CT]C BsaI 4487 GAGACC GGTCTC(1/5) BsaWI 4503 ACCGGA [AT]^CCGG[AT] BsoBI 4035 CTCGGG C^[CT]CG[GA]G Bst1107I 1873 GTATAC GTA^TAC BstZ17I 1873 GTATAC GTA^TAC CelII 454 GCTCAGC GC^T[ACGT]AGC DrdI 2383 GACCCCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 84 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 696 GATATC GAT^ATC EcoT22I 2265 ATGCAT ATGCA^T HaeII 4427 GGCGCT [GA]GCGC^[CT] HapII 4504 CCGG C^CGG Hin1I 994 GACGCC G[GA]^CG[CT]C HpaI 3117 GTTAAC GTT^AAC HpaII 4504 CCGG C^CGG MamI 3218 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 4504 CCGG C^CGG NruI 755 TCGCGA TCG^CGA NsiI 2265 ATGCAT ATGCA^T PflMI 3164 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2238 CACGTG CAC^GTG PmlI 2238 CACGTG CAC^GTG PshBI 4411 ATTAAT AT^TAAT Psp1406I 1822 AACGTT AA^CGTT SexAI 4490 ACCTGGT A^CC[AT]GGT SphI 1331 GCATGC GCATG^C Van91I 3164 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 3368 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 416 GGTACC G^GTACC 4444 GGTACC Asp718I 416 GGTACC G^GTACC 4444 GGTACC AspI 4234 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4489 GACCTGGTC BglII 1645 AGATCT A^GATCT 3738 AGATCT BsaAI 2238 CACGTG [CT]AC^GT[GA] 3896 CACGTA BseRI 151 CTCCTC GAGGAG(10/8) 4076 GAGGAG BsmBI 2349 CGTCTC CGTCTC(1/5) 2421 GAGACG BsrDI 1144 CATTGC GCAATG(2/0) 3599 CATTGC BstAPI 422 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2337 GCAGCTCCTGC BstEII 1366 GGTTACC G^GT[ACGT]ACC 4106 GGTAACC BstPI 1366 GGTTACC G^GT[ACGT]ACC 4106 GGTAACC BstXI 2687 CCAGAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4096 CCATCCTTCTGG CfoI 2722 GCGC GCG^C 4428 GCGC DraI 2599 TTTAAA TTT^AAA 2669 TTTAAA DraIII 3685 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 4478 CACGGTGTG EcoO65I 1366 GGTTACC G^GT[ACGT]ACC 4106 GGTAACC HgaI 994 GACGC GACGC(5/10) 69 GCGTC HhaI 2722 GCGC GCG^C 4428 GCGC HinP1I 2722 GCGC G^CGC 4428 GCGC HincII 724 GTCAAC GT[CT]^[GA]AC 3117 GTTAAC HindII 724 GTCAAC GT[CT]^[GA]AC 3117 GTTAAC HindIII 1003 AAGCTT A^AGCTT 3091 AAGCTT KpnI 416 GGTACC GGTAC^C 4444 GGTACC NcoI 2159 CCATGG C^CATGG 3169 CCATGG PflFI 4234 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4489 GACCTGGTC PpuMI 2325 AGGTCCC [GA]G^G[AT]CC[CT] 3997 AGGACCT SapI 1352 GAAGAGC GCTCTTC(1/4) 3917 GAAGAGC ScaI 1658 AGTACT AGT^ACT 2659 AGTACT StuI 3677 AGGCCT AGG^CCT 4624 AGGCCT TaqI 11 TCGA T^CGA 222 TCGA Tth111I 4234 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4489 GACCTGGTC TthHB8I 11 TCGA T^CGA 222 TCGA XbaI 2520 TCTAGA T^CTAGA 4024 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AluI AlwI AlwNI AocI ApoI AspHI AvaII BanI BanII BbsI BbvI BciVI BfaI BfrI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII