##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccIII AgeI Aor51HI AscI
AviII AvrII BalI BamHI BbeI
BclI BcnI BglI BlnI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
BstBI Cfr10I ClaI CpoI EaeI
EagI EclXI Eco47III Eco52I FbaI
FseI FspI KasI KspI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NspV PacI PaeR7I
PinAI PmeI PshAI PvuI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 1873 GTATAC GT^[AC][GT]AC
AclI 1822 AACGTT AA^CGTT
AcyI 994 GACGCC G[GA]^CG[CT]C
AflIII 3895 ACACGT A^C[GA][CT]GT
AhdI 84 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2235 GTGCAC G^TGCAC
ApaI 3260 GGGCCC GGGCC^C
ApaLI 2235 GTGCAC G^TGCAC
AseI 4411 ATTAAT AT^TAAT
AsnI 4411 ATTAAT AT^TAAT
Asp700I 3368 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 84 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4035 CTCGGG C^[CT]CG[GA]G
BbrPI 2238 CACGTG CAC^GTG
BbvCI 4073 GCTGAGG CCTCAGC(-5/-2)
BcgI 223 CGAGTTATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 454 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 454 GCTCAGC GC^T[ACGT]AGC
BsaBI 3218 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 994 GACGCC G[GA]^CG[CT]C
BsaI 4487 GAGACC GGTCTC(1/5)
BsaWI 4503 ACCGGA [AT]^CCGG[AT]
BsoBI 4035 CTCGGG C^[CT]CG[GA]G
Bst1107I 1873 GTATAC GTA^TAC
BstZ17I 1873 GTATAC GTA^TAC
CelII 454 GCTCAGC GC^T[ACGT]AGC
DrdI 2383 GACCCCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 84 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 696 GATATC GAT^ATC
EcoT22I 2265 ATGCAT ATGCA^T
HaeII 4427 GGCGCT [GA]GCGC^[CT]
HapII 4504 CCGG C^CGG
Hin1I 994 GACGCC G[GA]^CG[CT]C
HpaI 3117 GTTAAC GTT^AAC
HpaII 4504 CCGG C^CGG
MamI 3218 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 4504 CCGG C^CGG
NruI 755 TCGCGA TCG^CGA
NsiI 2265 ATGCAT ATGCA^T
PflMI 3164 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2238 CACGTG CAC^GTG
PmlI 2238 CACGTG CAC^GTG
PshBI 4411 ATTAAT AT^TAAT
Psp1406I 1822 AACGTT AA^CGTT
SexAI 4490 ACCTGGT A^CC[AT]GGT
SphI 1331 GCATGC GCATG^C
Van91I 3164 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3368 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 416 GGTACC G^GTACC
4444 GGTACC
Asp718I 416 GGTACC G^GTACC
4444 GGTACC
AspI 4234 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4489 GACCTGGTC
BglII 1645 AGATCT A^GATCT
3738 AGATCT
BsaAI 2238 CACGTG [CT]AC^GT[GA]
3896 CACGTA
BseRI 151 CTCCTC GAGGAG(10/8)
4076 GAGGAG
BsmBI 2349 CGTCTC CGTCTC(1/5)
2421 GAGACG
BsrDI 1144 CATTGC GCAATG(2/0)
3599 CATTGC
BstAPI 422 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2337 GCAGCTCCTGC
BstEII 1366 GGTTACC G^GT[ACGT]ACC
4106 GGTAACC
BstPI 1366 GGTTACC G^GT[ACGT]ACC
4106 GGTAACC
BstXI 2687 CCAGAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4096 CCATCCTTCTGG
CfoI 2722 GCGC GCG^C
4428 GCGC
DraI 2599 TTTAAA TTT^AAA
2669 TTTAAA
DraIII 3685 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4478 CACGGTGTG
EcoO65I 1366 GGTTACC G^GT[ACGT]ACC
4106 GGTAACC
HgaI 994 GACGC GACGC(5/10)
69 GCGTC
HhaI 2722 GCGC GCG^C
4428 GCGC
HinP1I 2722 GCGC G^CGC
4428 GCGC
HincII 724 GTCAAC GT[CT]^[GA]AC
3117 GTTAAC
HindII 724 GTCAAC GT[CT]^[GA]AC
3117 GTTAAC
HindIII 1003 AAGCTT A^AGCTT
3091 AAGCTT
KpnI 416 GGTACC GGTAC^C
4444 GGTACC
NcoI 2159 CCATGG C^CATGG
3169 CCATGG
PflFI 4234 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4489 GACCTGGTC
PpuMI 2325 AGGTCCC [GA]G^G[AT]CC[CT]
3997 AGGACCT
SapI 1352 GAAGAGC GCTCTTC(1/4)
3917 GAAGAGC
ScaI 1658 AGTACT AGT^ACT
2659 AGTACT
StuI 3677 AGGCCT AGG^CCT
4624 AGGCCT
TaqI 11 TCGA T^CGA
222 TCGA
Tth111I 4234 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4489 GACCTGGTC
TthHB8I 11 TCGA T^CGA
222 TCGA
XbaI 2520 TCTAGA T^CTAGA
4024 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanI BanII BbsI
BbvI BciVI BfaI BfrI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII