##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI ApaI AscI Asp718I AvrII
BbeI BciVI BlnI BsaI BseAI
BsiWI BspDI BspEI BssHII BssSI
Bst1107I BstXI BstZ17I ClaI CpoI
DrdI EcoRV EcoT22I FseI HpaI
KasI KpnI MluI MroI NarI
NruI NsiI PacI PinAI PshAI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SfoI SgrAI SnaBI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 349 GACGCC G[GA]^CG[CT]C
Alw44I 2022 GTGCAC G^TGCAC
Aor51HI 697 AGCGCT AGC^GCT
ApaLI 2022 GTGCAC G^TGCAC
AseI 4850 ATTAAT AT^TAAT
AsnI 4850 ATTAAT AT^TAAT
AspHI 2022 GTGCAC G[AT]GC[AT]^C
AviII 471 TGCGCA TGC^GCA
BbrPI 4290 CACGTG CAC^GTG
BcgI 1075 GCACTACAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 4020 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4020 GCTTAGC GC^T[ACGT]AGC
BsaAI 4290 CACGTG [CT]AC^GT[GA]
BsaHI 349 GACGCC G[GA]^CG[CT]C
BsiHKAI 2022 GTGCAC G[AT]GC[AT]^C
BsrBI 22 GAGCGG CCGCTC(-3/-3)
CelII 4020 GCTTAGC GC^T[ACGT]AGC
DraIII 5312 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 697 AGCGCT AGC^GCT
EcoNI 4230 CCTTCATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 5074 GAATTC G^AATTC
FspI 471 TGCGCA TGC^GCA
HgaI 349 GACGC GACGC(5/10)
Hin1I 349 GACGCC G[GA]^CG[CT]C
KspI 524 CCGCGG CCGC^GG
MslI 6056 CACAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 537 GCCGGC GCC^GGC
NdeI 5809 CATATG CA^TATG
NgoMIV 537 GCCGGC G^CCGGC
NheI 2513 GCTAGC G^CTAGC
NotI 167 GCGGCCGC GC^GGCCGC
PaeR7I 4704 CTCGAG C^TCGAG
PmaCI 4290 CACGTG CAC^GTG
PmeI 5959 GTTTAAAC GTTT^AAAC
PmlI 4290 CACGTG CAC^GTG
PshBI 4850 ATTAAT AT^TAAT
SacII 524 CCGCGG CCGC^GG
SapI 3621 GAAGAGC GCTCTTC(1/4)
SexAI 4481 ACCAGGT A^CC[AT]GGT
SmaI 2402 CCCGGG CCC^GGG
SphI 2058 GCATGC GCATG^C
XhoI 4704 CTCGAG C^TCGAG
XmaCI 2402 CCCGGG C^CCGGG
XmaI 2402 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflII 2729 CTTAAG C^TTAAG
2947 CTTAAG
AspI 349 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1017 GACTTGGTC
BalI 1878 TGGCCA TGG^CCA
2491 TGGCCA
BanII 425 GGGCTC G[GA]GC[CT]^C
1783 GGGCTC
BbvCI 707 CCTCAGC CCTCAGC(-5/-2)
1562 CCTCAGC
BclI 1509 TGATCA T^GATCA
6046 TGATCA
BfrI 2729 CTTAAG C^TTAAG
2947 CTTAAG
BsaBI 3050 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5649 GATAGCCATC
BsiEI 168 CGGCCG CG[GA][CT]^CG
535 CGGCCG
BsmBI 2238 CGTCTC CGTCTC(1/5)
3773 GAGACG
Bsp1407I 4352 TGTACA T^GTACA
5813 TGTACA
BspLU11I 2360 ACATGT A^CATGT
5955 ACATGT
BsrDI 4336 CATTGC GCAATG(2/0)
124 GCAATG
BsrFI 382 ACCGGC [GA]^CCGG[CT]
537 GCCGGC
BsrGI 4352 TGTACA T^GTACA
5813 TGTACA
BstAPI 763 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
799 GCAGGTCCTGC
BstEII 2039 GGTGACC G^GT[ACGT]ACC
4139 GGTGACC
BstPI 2039 GGTGACC G^GT[ACGT]ACC
4139 GGTGACC
Cfr10I 382 ACCGGC [GA]^CCGG[CT]
537 GCCGGC
EagI 168 CGGCCG C^GGCCG
535 CGGCCG
EclXI 168 CGGCCG C^GGCCG
535 CGGCCG
Eco52I 168 CGGCCG C^GGCCG
535 CGGCCG
EcoO65I 2039 GGTGACC G^GT[ACGT]ACC
4139 GGTGACC
FbaI 1509 TGATCA T^GATCA
6046 TGATCA
HaeII 577 GGCGCT [GA]GCGC^[CT]
697 AGCGCT
MamI 3050 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5649 GATAGCCATC
MluNI 1878 TGGCCA TGG^CCA
2491 TGGCCA
MscI 1878 TGGCCA TGG^CCA
2491 TGGCCA
PflFI 349 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1017 GACTTGGTC
PvuII 33 CAGCTG CAG^CTG
674 CAGCTG
SfiI 17 GGCCCGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1870 GGCCCGATTGGCC
SpeI 2645 ACTAGT A^CTAGT
5502 ACTAGT
SspBI 4352 TGTACA T^GTACA
5813 TGTACA
SspI 5344 AATATT AAT^ATT
5746 AATATT
Tth111I 349 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1017 GACTTGGTC
XbaI 4630 TCTAGA T^CTAGA
4981 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI AocI
ApoI Asp700I AspEI AvaI AvaII
BamHI BanI BbsI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BstBI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI Eam1105I EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MspA1I MspI MunI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI NspV PflMI
PleI PpuMI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfuI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI