##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccIII AclI AflIII AgeI ApaI
AscI AviII BamHI BbeI BbrPI
BciVI BclI BsaAI BsaBI BseAI
BsiWI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII FbaI FseI FspI HincII
HindII HpaI KasI KspI MamI
MfeI MluI MroI MunI NarI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 737 GGTACC G^GTACC
AflII 2925 CTTAAG C^TTAAG
AhdI 2814 GACCTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 3383 GGATC GGATC(4/5)
Aor51HI 4797 AGCGCT AGC^GCT
Asp700I 334 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 737 GGTACC G^GTACC
AspEI 2814 GACCTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4441 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2145 CCTAGG C^CTAGG
BbvCI 4268 CCTCAGC CCTCAGC(-5/-2)
BcgI 4855 GCACTGGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2925 CTTAAG C^TTAAG
BglI 2433 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2145 CCTAGG C^CTAGG
BsaWI 5696 ACCGGA [AT]^CCGG[AT]
BsgI 4222 GTGCAG GTGCAG(16/14)
BsiEI 2446 CGGCCG CG[GA][CT]^CG
BsmI 4133 GCATTC GAATGC(1/-1)
BsrFI 2448 GCCGGC [GA]^CCGG[CT]
BssSI 2134 CACGAG CACGAG(-5/-1)
Cfr10I 2448 GCCGGC [GA]^CCGG[CT]
DraI 3063 TTTAAA TTT^AAA
EagI 2446 CGGCCG C^GGCCG
Eam1105I 2814 GACCTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2446 CGGCCG C^GGCCG
Eco47III 4797 AGCGCT AGC^GCT
Eco52I 2446 CGGCCG C^GGCCG
EcoRI 3958 GAATTC G^AATTC
EcoRV 1096 GATATC GAT^ATC
EcoT22I 3542 ATGCAT ATGCA^T
KpnI 737 GGTACC GGTAC^C
NaeI 2448 GCCGGC GCC^GGC
NgoMIV 2448 GCCGGC G^CCGGC
NheI 2203 GCTAGC G^CTAGC
NsiI 3542 ATGCAT ATGCA^T
PaeR7I 2777 CTCGAG C^TCGAG
PflFI 4441 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfiI 2432 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1158 CCCGGG CCC^GGG
Tth111I 4441 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5157 TCTAGA T^CTAGA
XhoI 2777 CTCGAG C^TCGAG
XmaCI 1158 CCCGGG C^CCGGG
XmaI 1158 CCCGGG C^CCGGG
XmnI 334 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 1228 GACGTC GACGT^C
2980 GACGTC
Alw44I 534 GTGCAC G^TGCAC
3567 GTGCAC
AocI 4524 CCTCAGG CC^T[ACGT]AGG
4618 CCTAAGG
ApaLI 534 GTGCAC G^TGCAC
3567 GTGCAC
AseI 5306 ATTAAT AT^TAAT
5323 ATTAAT
AsnI 5306 ATTAAT AT^TAAT
5323 ATTAAT
BalI 297 TGGCCA TGG^CCA
4098 TGGCCA
BanI 737 GGTACC G^G[CT][GA]CC
2583 GGCACC
BlpI 1546 GCTGAGC GC^T[ACGT]AGC
1858 GCTGAGC
Bpu1102I 1546 GCTGAGC GC^T[ACGT]AGC
1858 GCTGAGC
Bsp1407I 4011 TGTACA T^GTACA
5189 TGTACA
BspHI 2354 TCATGA T^CATGA
3952 TCATGA
BsrGI 4011 TGTACA T^GTACA
5189 TGTACA
BstAPI 1324 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3702 GCATTGCCTGC
BstEII 1006 GGTTACC G^GT[ACGT]ACC
2522 GGTGACC
BstPI 1006 GGTTACC G^GT[ACGT]ACC
2522 GGTGACC
Bsu36I 4524 CCTCAGG CC^T[ACGT]AGG
4618 CCTAAGG
CelII 1546 GCTGAGC GC^T[ACGT]AGC
1858 GCTGAGC
Eco81I 4524 CCTCAGG CC^T[ACGT]AGG
4618 CCTAAGG
EcoO65I 1006 GGTTACC G^GT[ACGT]ACC
2522 GGTGACC
HaeII 2101 AGCGCC [GA]GCGC^[CT]
4797 AGCGCT
HindIII 2008 AAGCTT A^AGCTT
3925 AAGCTT
MluNI 297 TGGCCA TGG^CCA
4098 TGGCCA
MscI 297 TGGCCA TGG^CCA
4098 TGGCCA
NdeI 994 CATATG CA^TATG
3043 CATATG
PshBI 5306 ATTAAT AT^TAAT
5323 ATTAAT
RcaI 2354 TCATGA T^CATGA
3952 TCATGA
SacI 968 GAGCTC GAGCT^C
3835 GAGCTC
SapI 30 GAAGAGC GCTCTTC(1/4)
711 GAAGAGC
ScaI 4579 AGTACT AGT^ACT
4891 AGTACT
SspBI 4011 TGTACA T^GTACA
5189 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwNI ApoI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII