##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII AvrII BbrPI BcgI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BsrFI BssHII BstBI BstEII BstPI
BstXI CelII Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI FseI FspI KpnI
KspI MamI MluI MroI NaeI
NgoMIV NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SexAI SfiI SfuI SgrAI
SnaBI StuI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 25 CGCG CG^CG
AflII 2589 CTTAAG C^TTAAG
AocI 3771 CCTTAGG CC^T[ACGT]AGG
ApaI 4445 GGGCCC GGGCC^C
AspI 2095 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 46 GGCGCC G^G[CT][GA]CC
BbeI 46 GGCGCC GGCGC^C
BbvCI 1836 CCTCAGC CCTCAGC(-5/-2)
BclI 1444 TGATCA T^GATCA
BfrI 2589 CTTAAG C^TTAAG
BglI 1074 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
Bsp1407I 2183 TGTACA T^GTACA
BspHI 5087 TCATGA T^CATGA
BsrBI 3743 GAGCGG CCGCTC(-3/-3)
BsrGI 2183 TGTACA T^GTACA
BssSI 3023 CTCGTG CACGAG(-5/-1)
Bst1107I 2465 GTATAC GTA^TAC
BstUI 25 CGCG CG^CG
BstZ17I 2465 GTATAC GTA^TAC
Bsu36I 3771 CCTTAGG CC^T[ACGT]AGG
Eco81I 3771 CCTTAGG CC^T[ACGT]AGG
EcoNI 3037 CCTTATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1255 GATATC GAT^ATC
FbaI 1444 TGATCA T^GATCA
HaeII 46 GGCGCC [GA]GCGC^[CT]
HgaI 1323 GCGTC GACGC(5/10)
HindIII 3920 AAGCTT A^AGCTT
KasI 46 GGCGCC G^GCGCC
MaeII 3755 ACGT A^CGT
MfeI 4586 CAATTG C^AATTG
MunI 4586 CAATTG C^AATTG
MvnI 25 CGCG CG^CG
NarI 46 GGCGCC GG^CGCC
NdeI 2537 CATATG CA^TATG
PaeR7I 1166 CTCGAG C^TCGAG
PflFI 2095 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1622 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5087 TCATGA T^CATGA
SapI 4905 GAAGAGC GCTCTTC(1/4)
SbfI 2826 CCTGCAGG CCTGCA^GG
ScaI 3642 AGTACT AGT^ACT
SfoI 46 GGCGCC GGC^GCC
SmaI 1078 CCCGGG CCC^GGG
Sse8387I 2826 CCTGCAGG CCTGCA^GG
SspBI 2183 TGTACA T^GTACA
SwaI 2732 ATTTAAAT ATTT^AAAT
Tth111I 2095 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2536 CCATATGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1166 CTCGAG C^TCGAG
XmaCI 1078 CCCGGG C^CCGGG
XmaI 1078 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 2019 GTAGAC GT^[AC][GT]AC
2465 GTATAC
AcyI 46 GGCGCC G[GA]^CG[CT]C
1322 GGCGTC
AflIII 716 ACATGT A^C[GA][CT]GT
1688 ACATGT
AhdI 2781 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3435 GACAAAAAGTC
Alw44I 1610 GTGCAC G^TGCAC
3671 GTGCAC
ApaLI 1610 GTGCAC G^TGCAC
3671 GTGCAC
Asp700I 794 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
854 GAAATTGTTC
AspEI 2781 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3435 GACAAAAAGTC
AvaI 1078 CCCGGG C^[CT]CG[GA]G
1166 CTCGAG
BamHI 2499 GGATCC G^GATCC
3356 GGATCC
BanII 3543 GGGCTC G[GA]GC[CT]^C
4445 GGGCCC
BbsI 1137 GAAGAC GAAGAC(2/6)
1192 GAAGAC
BpuAI 1137 GAAGAC GAAGAC(2/6)
1192 GAAGAC
BsaHI 46 GGCGCC G[GA]^CG[CT]C
1322 GGCGTC
BsgI 3760 CTGCAC GTGCAG(16/14)
4566 CTGCAC
BsmBI 3752 GAGACG CGTCTC(1/5)
3779 GAGACG
BsoBI 1078 CCCGGG C^[CT]CG[GA]G
1166 CTCGAG
BspLU11I 716 ACATGT A^CATGT
1688 ACATGT
BstAPI 1980 GCAGTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4419 GCATTGGTTGC
DsaI 2197 CCATGG C^C[GA][CT]GG
4163 CCATGG
Eam1105I 2781 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3435 GACAAAAAGTC
EcoT22I 2455 ATGCAT ATGCA^T
4405 ATGCAT
FauI 15 CCCGC CCCGC(4/6)
36 GCGGG
Hin1I 46 GGCGCC G[GA]^CG[CT]C
1322 GGCGTC
HpaI 1106 GTTAAC GTT^AAC
1673 GTTAAC
MspA1I 1839 CAGCTG C[AC]G^C[GT]G
2614 CAGCTG
NcoI 2197 CCATGG C^CATGG
4163 CCATGG
NheI 4882 GCTAGC G^CTAGC
4896 GCTAGC
NsiI 2455 ATGCAT ATGCA^T
4405 ATGCAT
PvuII 1839 CAGCTG CAG^CTG
2614 CAGCTG
SpeI 2159 ACTAGT A^CTAGT
2853 ACTAGT
SphI 1886 GCATGC GCATG^C
1985 GCATGC
XmnI 794 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
854 GAAATTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BbvI BciVI BcnI BfaI BglII
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII