##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AclI AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AvaI AviII AvrII BbeI BcgI
BciVI BglII BlnI BsiWI BsoBI
BssHII Bst1107I BstAPI BstZ17I Bsu36I
CpoI DrdI Eco47III Eco81I FseI
FspI KasI KspI MfeI MluI
MunI NarI NheI NotI NruI
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfoI SgrAI SmaI
SnaBI Sse8387I StuI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 3148 GACGTC GACGT^C
AccI 3317 GTCTAC GT^[AC][GT]AC
AccIII 934 TCCGGA T^CCGGA
AflII 1806 CTTAAG C^TTAAG
AflIII 1180 ACATGT A^C[GA][CT]GT
AhdI 4969 GACGAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 4969 GACGAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 782 GGATCC G^GATCC
BbrPI 1074 CACGTG CAC^GTG
BbvCI 1833 GCTGAGG CCTCAGC(-5/-2)
BclI 2263 TGATCA T^GATCA
BfrI 1806 CTTAAG C^TTAAG
BglI 417 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2431 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2431 GCTTAGC GC^T[ACGT]AGC
BsaWI 934 TCCGGA [AT]^CCGG[AT]
BseAI 934 TCCGGA T^CCGGA
BsiEI 349 CGGCCG CG[GA][CT]^CG
BspDI 1290 ATCGAT AT^CGAT
BspEI 934 TCCGGA T^CCGGA
BspLU11I 1180 ACATGT A^CATGT
BstYI 782 GGATCC [GA]^GATC[CT]
CelII 2431 GCTTAGC GC^T[ACGT]AGC
ClaI 1290 ATCGAT AT^CGAT
EagI 349 CGGCCG C^GGCCG
Eam1105I 4969 GACGAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 349 CGGCCG C^GGCCG
Eco52I 349 CGGCCG C^GGCCG
EcoRI 1869 GAATTC G^AATTC
EcoRV 4401 GATATC GAT^ATC
EcoT22I 3786 ATGCAT ATGCA^T
FbaI 2263 TGATCA T^GATCA
MflI 782 GGATCC [GA]^GATC[CT]
MroI 934 TCCGGA T^CCGGA
NaeI 3088 GCCGGC GCC^GGC
NdeI 5459 CATATG CA^TATG
NgoMIV 3088 GCCGGC G^CCGGC
NsiI 3786 ATGCAT ATGCA^T
PflMI 438 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1074 CACGTG CAC^GTG
PmlI 1074 CACGTG CAC^GTG
PshAI 684 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1633 CTGCAG CTGCA^G
SacI 1233 GAGCTC GAGCT^C
SapI 4767 GAAGAGC GCTCTTC(1/4)
ScaI 3197 AGTACT AGT^ACT
SfiI 416 GGCCTTTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 4588 ACTAGT A^CTAGT
SphI 2662 GCATGC GCATG^C
Van91I 438 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 782 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1069 GGTACC G^GTACC
1104 GGTACC
AccII 1278 CGCG CG^CG
1409 CGCG
Asp718I 1069 GGTACC G^GTACC
1104 GGTACC
BsaBI 110 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
731 GATGAAAATC
BsgI 1448 CTGCAC GTGCAG(16/14)
3909 GTGCAG
BsmI 3388 GCATTC GAATGC(1/-1)
4120 GCATTC
BspHI 1894 TCATGA T^CATGA
5039 TCATGA
BsrBI 3374 CCGCTC CCGCTC(-3/-3)
5067 GAGCGG
BsrFI 1458 ACCGGC [GA]^CCGG[CT]
3088 GCCGGC
BssSI 202 CACGAG CACGAG(-5/-1)
4250 CTCGTG
BstBI 121 TTCGAA TT^CGAA
4516 TTCGAA
BstEII 2412 GGTGACC G^GT[ACGT]ACC
4346 GGTTACC
BstPI 2412 GGTGACC G^GT[ACGT]ACC
4346 GGTTACC
BstUI 1278 CGCG CG^CG
1409 CGCG
Cfr10I 1458 ACCGGC [GA]^CCGG[CT]
3088 GCCGGC
EcoNI 494 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
656 CCTTAAAGAGG
EcoO65I 2412 GGTGACC G^GT[ACGT]ACC
4346 GGTTACC
HaeII 1371 AGCGCC [GA]GCGC^[CT]
2877 AGCGCC
HindIII 2463 AAGCTT A^AGCTT
5417 AAGCTT
HpaI 360 GTTAAC GTT^AAC
1683 GTTAAC
KpnI 1069 GGTACC GGTAC^C
1104 GGTACC
MamI 110 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
731 GATGAAAATC
MvnI 1278 CGCG CG^CG
1409 CGCG
NcoI 1540 CCATGG C^CATGG
3346 CCATGG
NspV 121 TTCGAA TT^CGAA
4516 TTCGAA
RcaI 1894 TCATGA T^CATGA
5039 TCATGA
SfuI 121 TTCGAA TT^CGAA
4516 TTCGAA
XbaI 692 TCTAGA T^CTAGA
1649 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI Asp700I AspHI AspI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XmnI