################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AflIII ApoI AscI AseI AsnI Asp700I Asp718I BamHI BbeI BciVI BclI BfrI BsaBI BsiWI Bsp1407I BspDI BspLU11I BsrGI BssHII BstBI ClaI CpoI DraI EcoRI FbaI FseI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SmaI SpeI Sse8387I SspBI SspI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 2649 GTATAC GT^[AC][GT]AC AgeI 2582 ACCGGT A^CCGGT AhdI 4763 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3119 AGCGCT AGC^GCT AspEI 4763 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 4538 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 550 CCTAGG C^CTAGG BbsI 1949 GAAGAC GAAGAC(2/6) BbvCI 538 CCTCAGC CCTCAGC(-5/-2) BlnI 550 CCTAGG C^CTAGG BpuAI 1949 GAAGAC GAAGAC(2/6) BsmI 1204 GCATTC GAATGC(1/-1) BsrBI 2677 CCGCTC CCGCTC(-3/-3) Bst1107I 2649 GTATAC GTA^TAC BstZ17I 2649 GTATAC GTA^TAC Eam1105I 4763 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3119 AGCGCT AGC^GCT EcoRV 1490 GATATC GAT^ATC EcoT22I 4774 ATGCAT ATGCA^T HindIII 3098 AAGCTT A^AGCTT HpaI 4481 GTTAAC GTT^AAC NsiI 4774 ATGCAT ATGCA^T PflFI 4538 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2582 ACCGGT A^CCGGT PvuII 994 CAGCTG CAG^CTG SapI 2935 GAAGAGC GCTCTTC(1/4) SexAI 3264 ACCTGGT A^CC[AT]GGT SgrAI 2581 CACCGGTG C[GA]^CCGG[CT]G SmlI 3215 CTCAAG C^T[CT][GA]AG SnaBI 4630 TACGTA TAC^GTA StuI 3222 AGGCCT AGG^CCT Tth111I 4538 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2177 TCTAGA T^CTAGA XcmI 2268 CCATCACCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 4255 CGCG CG^CG 4535 CGCG AccIII 363 TCCGGA T^CCGGA 1341 TCCGGA AocI 2552 CCTCAGG CC^T[ACGT]AGG 3320 CCTGAGG AviII 3 TGCGCA TGC^GCA 594 TGCGCA BbrPI 35 CACGTG CAC^GTG 680 CACGTG BglI 3310 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4195 GCCCACCTGGC BseAI 363 TCCGGA T^CCGGA 1341 TCCGGA BspEI 363 TCCGGA T^CCGGA 1341 TCCGGA BspHI 705 TCATGA T^CATGA 1759 TCATGA BsrFI 2582 ACCGGT [GA]^CCGG[CT] 3239 ACCGGC BstUI 4255 CGCG CG^CG 4535 CGCG Bsu36I 2552 CCTCAGG CC^T[ACGT]AGG 3320 CCTGAGG Cfr10I 2582 ACCGGT [GA]^CCGG[CT] 3239 ACCGGC DraIII 35 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG 3187 CACTTGGTG EagI 54 CGGCCG C^GGCCG 166 CGGCCG EarI 2153 GAAGAG CTCTTC(1/4) 2935 GAAGAG EclXI 54 CGGCCG C^GGCCG 166 CGGCCG Eco52I 54 CGGCCG C^GGCCG 166 CGGCCG Eco81I 2552 CCTCAGG CC^T[ACGT]AGG 3320 CCTGAGG FspI 3 TGCGCA TGC^GCA 594 TGCGCA Ksp632I 2153 GAAGAG CTCTTC(1/4) 2935 GAAGAG MroI 363 TCCGGA T^CCGGA 1341 TCCGGA MvnI 4255 CGCG CG^CG 4535 CGCG PmaCI 35 CACGTG CAC^GTG 680 CACGTG PmlI 35 CACGTG CAC^GTG 680 CACGTG RcaI 705 TCATGA T^CATGA 1759 TCATGA SacI 1187 GAGCTC GAGCT^C 2741 GAGCTC TaqI 390 TCGA T^CGA 1350 TCGA TfiI 2329 GATTC G^A[AT]TC 3134 GAATC TthHB8I 390 TCGA T^CGA 1350 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AciI AcyI AfaI AluI Alw44I AlwI AlwNI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbvI BcgI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SphI StyI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII