##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII ApoI AscI AseI AsnI
Asp700I Asp718I BamHI BbeI BciVI
BclI BfrI BsaBI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssHII BstBI
ClaI CpoI DraI EcoRI FbaI
FseI KasI KpnI KspI MamI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SmaI SpeI Sse8387I SspBI
SspI SwaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2649 GTATAC GT^[AC][GT]AC
AgeI 2582 ACCGGT A^CCGGT
AhdI 4763 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3119 AGCGCT AGC^GCT
AspEI 4763 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4538 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 550 CCTAGG C^CTAGG
BbsI 1949 GAAGAC GAAGAC(2/6)
BbvCI 538 CCTCAGC CCTCAGC(-5/-2)
BlnI 550 CCTAGG C^CTAGG
BpuAI 1949 GAAGAC GAAGAC(2/6)
BsmI 1204 GCATTC GAATGC(1/-1)
BsrBI 2677 CCGCTC CCGCTC(-3/-3)
Bst1107I 2649 GTATAC GTA^TAC
BstZ17I 2649 GTATAC GTA^TAC
Eam1105I 4763 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3119 AGCGCT AGC^GCT
EcoRV 1490 GATATC GAT^ATC
EcoT22I 4774 ATGCAT ATGCA^T
HindIII 3098 AAGCTT A^AGCTT
HpaI 4481 GTTAAC GTT^AAC
NsiI 4774 ATGCAT ATGCA^T
PflFI 4538 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2582 ACCGGT A^CCGGT
PvuII 994 CAGCTG CAG^CTG
SapI 2935 GAAGAGC GCTCTTC(1/4)
SexAI 3264 ACCTGGT A^CC[AT]GGT
SgrAI 2581 CACCGGTG C[GA]^CCGG[CT]G
SmlI 3215 CTCAAG C^T[CT][GA]AG
SnaBI 4630 TACGTA TAC^GTA
StuI 3222 AGGCCT AGG^CCT
Tth111I 4538 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2177 TCTAGA T^CTAGA
XcmI 2268 CCATCACCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 4255 CGCG CG^CG
4535 CGCG
AccIII 363 TCCGGA T^CCGGA
1341 TCCGGA
AocI 2552 CCTCAGG CC^T[ACGT]AGG
3320 CCTGAGG
AviII 3 TGCGCA TGC^GCA
594 TGCGCA
BbrPI 35 CACGTG CAC^GTG
680 CACGTG
BglI 3310 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4195 GCCCACCTGGC
BseAI 363 TCCGGA T^CCGGA
1341 TCCGGA
BspEI 363 TCCGGA T^CCGGA
1341 TCCGGA
BspHI 705 TCATGA T^CATGA
1759 TCATGA
BsrFI 2582 ACCGGT [GA]^CCGG[CT]
3239 ACCGGC
BstUI 4255 CGCG CG^CG
4535 CGCG
Bsu36I 2552 CCTCAGG CC^T[ACGT]AGG
3320 CCTGAGG
Cfr10I 2582 ACCGGT [GA]^CCGG[CT]
3239 ACCGGC
DraIII 35 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3187 CACTTGGTG
EagI 54 CGGCCG C^GGCCG
166 CGGCCG
EarI 2153 GAAGAG CTCTTC(1/4)
2935 GAAGAG
EclXI 54 CGGCCG C^GGCCG
166 CGGCCG
Eco52I 54 CGGCCG C^GGCCG
166 CGGCCG
Eco81I 2552 CCTCAGG CC^T[ACGT]AGG
3320 CCTGAGG
FspI 3 TGCGCA TGC^GCA
594 TGCGCA
Ksp632I 2153 GAAGAG CTCTTC(1/4)
2935 GAAGAG
MroI 363 TCCGGA T^CCGGA
1341 TCCGGA
MvnI 4255 CGCG CG^CG
4535 CGCG
PmaCI 35 CACGTG CAC^GTG
680 CACGTG
PmlI 35 CACGTG CAC^GTG
680 CACGTG
RcaI 705 TCATGA T^CATGA
1759 TCATGA
SacI 1187 GAGCTC GAGCT^C
2741 GAGCTC
TaqI 390 TCGA T^CGA
1350 TCGA
TfiI 2329 GATTC G^A[AT]TC
3134 GAATC
TthHB8I 390 TCGA T^CGA
1350 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcyI AfaI AluI Alw44I
AlwI AlwNI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcgI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SphI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII