##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AflII AgeI Alw44I ApaLI
AscI AseI AsnI Asp718I AviII
AvrII BbvCI BciVI BfrI BlnI
BsiEI BsiWI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI EagI EclXI
Eco52I EcoNI EcoO65I EcoT22I FseI
FspI KpnI MfeI MunI NdeI
NheI NotI NruI NsiI NspV
PinAI PmeI PshBI PvuI RsrII
SalI SfiI SfuI SgrAI SnaBI
SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 2291 GACGTC GACGT^C
AccIII 930 TCCGGA T^CCGGA
AclI 2098 AACGTT AA^CGTT
AhdI 2667 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1358 CCTCAGG CC^T[ACGT]AGG
Aor51HI 508 AGCGCT AGC^GCT
ApaI 645 GGGCCC GGGCC^C
AspEI 2667 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1404 GGCGCC GGCGC^C
BglI 101 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2793 AGATCT A^GATCT
BsaBI 1059 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 930 TCCGGA T^CCGGA
BspEI 930 TCCGGA T^CCGGA
BspLU11I 4735 ACATGT A^CATGT
BsrDI 3766 GCAATG GCAATG(2/0)
Bsu36I 1358 CCTCAGG CC^T[ACGT]AGG
DraI 3011 TTTAAA TTT^AAA
Eam1105I 2667 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 508 AGCGCT AGC^GCT
Eco81I 1358 CCTCAGG CC^T[ACGT]AGG
EcoRI 2975 GAATTC G^AATTC
EcoRV 1286 GATATC GAT^ATC
HpaI 884 GTTAAC GTT^AAC
KasI 1404 GGCGCC G^GCGCC
KspI 3085 CCGCGG CCGC^GG
MamI 1059 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4155 ACGCGT A^CGCGT
MroI 930 TCCGGA T^CCGGA
NaeI 3599 GCCGGC GCC^GGC
NarI 1404 GGCGCC GG^CGCC
NgoMIV 3599 GCCGGC G^CCGGC
PacI 3215 TTAATTAA TTAAT^TAA
PaeR7I 3281 CTCGAG C^TCGAG
Psp1406I 2098 AACGTT AA^CGTT
SacI 530 GAGCTC GAGCT^C
SacII 3085 CCGCGG CCGC^GG
SbfI 414 CCTGCAGG CCTGCA^GG
ScaI 1742 AGTACT AGT^ACT
SexAI 1269 ACCAGGT A^CC[AT]GGT
SfoI 1404 GGCGCC GGC^GCC
SmaI 2092 CCCGGG CCC^GGG
SpeI 4827 ACTAGT A^CTAGT
SphI 1368 GCATGC GCATG^C
Sse8387I 414 CCTGCAGG CCTGCA^GG
StuI 696 AGGCCT AGG^CCT
XcmI 5168 CCACTGGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3281 CTCGAG C^TCGAG
XmaCI 2092 CCCGGG C^CCGGG
XmaI 2092 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1244 GTCTAC GT^[AC][GT]AC
3395 GTCTAC
AcyI 1404 GGCGCC G[GA]^CG[CT]C
2291 GACGTC
BcgI 2432 GCAGTGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3201 GCAAAGCTCTCG
BclI 2898 TGATCA T^GATCA
3582 TGATCA
BlpI 896 GCTGAGC GC^T[ACGT]AGC
3358 GCTGAGC
Bpu1102I 896 GCTGAGC GC^T[ACGT]AGC
3358 GCTGAGC
BsaHI 1404 GGCGCC G[GA]^CG[CT]C
2291 GACGTC
BsgI 626 CTGCAC GTGCAG(16/14)
1855 GTGCAG
BsmI 300 GCATTC GAATGC(1/-1)
1750 GCATTC
BspMI 4919 ACCTGC ACCTGC(4/8)
2472 GCAGGT
BsrBI 3641 GAGCGG CCGCTC(-3/-3)
4530 GAGCGG
BsrFI 2829 ACCGGC [GA]^CCGG[CT]
3599 GCCGGC
BssSI 533 CTCGTG CACGAG(-5/-1)
2615 CTCGTG
BstAPI 3349 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4589 GCAGGGTCTGC
BstXI 305 CCAGGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1102 CCAGGTGGATGG
CelII 896 GCTGAGC GC^T[ACGT]AGC
3358 GCTGAGC
Cfr10I 2829 ACCGGC [GA]^CCGG[CT]
3599 GCCGGC
DraIII 254 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1849 CACAGTGTG
DrdI 3242 GACCTGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3709 GACGAGCGAGTC
FbaI 2898 TGATCA T^GATCA
3582 TGATCA
Hin1I 1404 GGCGCC G[GA]^CG[CT]C
2291 GACGTC
MslI 2157 CACTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2480 CATTCCGATG
SspI 936 AATATT AAT^ATT
5249 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbrPI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PshAI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII XmnI