##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII Acc65I AccI AgeI AocI
Aor51HI ApaI AscI Asp718I AviII
BcgI BsiWI BsmBI BspDI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I FseI
FspI KpnI NheI NotI NspV
PacI PinAI PmeI PshAI PvuI
RsrII SalI SbfI ScaI SfuI
SgrAI SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 929 TCCGGA T^CCGGA
AflII 3978 CTTAAG C^TTAAG
AhdI 94 GACCGATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 380 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 933 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 94 GACCGATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3697 CCTAGG C^CTAGG
BamHI 366 GGATCC G^GATCC
BbeI 267 GGCGCC GGCGC^C
BbrPI 1320 CACGTG CAC^GTG
BbvCI 4539 CCTCAGC CCTCAGC(-5/-2)
BciVI 3117 GTATCC GTATCC(6/5)
BfrI 3978 CTTAAG C^TTAAG
BglII 2850 AGATCT A^GATCT
BlnI 3697 CCTAGG C^CTAGG
BlpI 2661 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2661 GCTGAGC GC^T[ACGT]AGC
BseAI 929 TCCGGA T^CCGGA
BsiEI 279 CGACCG CG[GA][CT]^CG
BspEI 929 TCCGGA T^CCGGA
BsrDI 1876 CATTGC GCAATG(2/0)
BssHII 790 GCGCGC G^CGCGC
BstAPI 379 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 4044 CCATCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2661 GCTGAGC GC^T[ACGT]AGC
DraIII 2282 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 74 GACCTCCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 94 GACCGATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4964 GAATTC G^AATTC
EcoRV 3489 GATATC GAT^ATC
HindIII 4995 AAGCTT A^AGCTT
HpaI 4114 GTTAAC GTT^AAC
KasI 267 GGCGCC G^GCGCC
KspI 701 CCGCGG CCGC^GG
MfeI 1497 CAATTG C^AATTG
MluI 935 ACGCGT A^CGCGT
MroI 929 TCCGGA T^CCGGA
MunI 1497 CAATTG C^AATTG
NarI 267 GGCGCC GG^CGCC
NdeI 2313 CATATG CA^TATG
NruI 276 TCGCGA TCG^CGA
PaeR7I 2331 CTCGAG C^TCGAG
PflMI 3032 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1320 CACGTG CAC^GTG
PmlI 1320 CACGTG CAC^GTG
SacI 402 GAGCTC GAGCT^C
SacII 701 CCGCGG CCGC^GG
SapI 744 GAAGAGC GCTCTTC(1/4)
SexAI 1265 ACCAGGT A^CC[AT]GGT
SfiI 810 GGCCAGTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 267 GGCGCC GGC^GCC
SpeI 4009 ACTAGT A^CTAGT
Van91I 3032 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5050 TCTAGA T^CTAGA
XcmI 2989 CCACTCTCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2331 CTCGAG C^TCGAG
XmnI 933 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AclI 1336 AACGTT AA^CGTT
2415 AACGTT
AcyI 233 GACGCC G[GA]^CG[CT]C
267 GGCGCC
Alw44I 261 GTGCAC G^TGCAC
2318 GTGCAC
ApaLI 261 GTGCAC G^TGCAC
2318 GTGCAC
AseI 1867 ATTAAT AT^TAAT
1953 ATTAAT
AsnI 1867 ATTAAT AT^TAAT
1953 ATTAAT
AspI 540 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3354 GACATAGTC
BalI 22 TGGCCA TGG^CCA
809 TGGCCA
BglI 700 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
811 GCCAGTTCGGC
BsaAI 912 CACGTA [CT]AC^GT[GA]
1320 CACGTG
BsaHI 233 GACGCC G[GA]^CG[CT]C
267 GGCGCC
BsaI 994 GAGACC GGTCTC(1/5)
372 GGTCTC
BsaWI 369 TCCGGT [AT]^CCGG[AT]
929 TCCGGA
BsmI 2746 GCATTC GAATGC(1/-1)
4634 GCATTC
BsrBI 272 CCGCTC CCGCTC(-3/-3)
355 CCGCTC
BsrFI 409 GCCGGC [GA]^CCGG[CT]
864 GCCGGC
Cfr10I 409 GCCGGC [GA]^CCGG[CT]
864 GCCGGC
EcoT22I 2094 ATGCAT ATGCA^T
4092 ATGCAT
Hin1I 233 GACGCC G[GA]^CG[CT]C
267 GGCGCC
MluNI 22 TGGCCA TGG^CCA
809 TGGCCA
MscI 22 TGGCCA TGG^CCA
809 TGGCCA
NaeI 409 GCCGGC GCC^GGC
864 GCCGGC
NcoI 47 CCATGG C^CATGG
2551 CCATGG
NgoMIV 409 GCCGGC G^CCGGC
864 GCCGGC
NsiI 2094 ATGCAT ATGCA^T
4092 ATGCAT
PflFI 540 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3354 GACATAGTC
PshBI 1867 ATTAAT AT^TAAT
1953 ATTAAT
Psp1406I 1336 AACGTT AA^CGTT
2415 AACGTT
StuI 4646 AGGCCT AGG^CCT
4873 AGGCCT
Tth111I 540 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3354 GACATAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI