################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AatII Acc65I AccI AgeI AocI Aor51HI ApaI AscI Asp718I AviII BcgI BsiWI BsmBI BspDI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI EagI EclXI Eco47III Eco52I Eco81I EcoO65I FseI FspI KpnI NheI NotI NspV PacI PinAI PmeI PshAI PvuI RsrII SalI SbfI ScaI SfuI SgrAI SnaBI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 929 TCCGGA T^CCGGA AflII 3978 CTTAAG C^TTAAG AhdI 94 GACCGATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 380 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 933 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 94 GACCGATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3697 CCTAGG C^CTAGG BamHI 366 GGATCC G^GATCC BbeI 267 GGCGCC GGCGC^C BbrPI 1320 CACGTG CAC^GTG BbvCI 4539 CCTCAGC CCTCAGC(-5/-2) BciVI 3117 GTATCC GTATCC(6/5) BfrI 3978 CTTAAG C^TTAAG BglII 2850 AGATCT A^GATCT BlnI 3697 CCTAGG C^CTAGG BlpI 2661 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2661 GCTGAGC GC^T[ACGT]AGC BseAI 929 TCCGGA T^CCGGA BsiEI 279 CGACCG CG[GA][CT]^CG BspEI 929 TCCGGA T^CCGGA BsrDI 1876 CATTGC GCAATG(2/0) BssHII 790 GCGCGC G^CGCGC BstAPI 379 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 4044 CCATCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2661 GCTGAGC GC^T[ACGT]AGC DraIII 2282 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 74 GACCTCCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 94 GACCGATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 4964 GAATTC G^AATTC EcoRV 3489 GATATC GAT^ATC HindIII 4995 AAGCTT A^AGCTT HpaI 4114 GTTAAC GTT^AAC KasI 267 GGCGCC G^GCGCC KspI 701 CCGCGG CCGC^GG MfeI 1497 CAATTG C^AATTG MluI 935 ACGCGT A^CGCGT MroI 929 TCCGGA T^CCGGA MunI 1497 CAATTG C^AATTG NarI 267 GGCGCC GG^CGCC NdeI 2313 CATATG CA^TATG NruI 276 TCGCGA TCG^CGA PaeR7I 2331 CTCGAG C^TCGAG PflMI 3032 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1320 CACGTG CAC^GTG PmlI 1320 CACGTG CAC^GTG SacI 402 GAGCTC GAGCT^C SacII 701 CCGCGG CCGC^GG SapI 744 GAAGAGC GCTCTTC(1/4) SexAI 1265 ACCAGGT A^CC[AT]GGT SfiI 810 GGCCAGTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 267 GGCGCC GGC^GCC SpeI 4009 ACTAGT A^CTAGT Van91I 3032 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 5050 TCTAGA T^CTAGA XcmI 2989 CCACTCTCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2331 CTCGAG C^TCGAG XmnI 933 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AclI 1336 AACGTT AA^CGTT 2415 AACGTT AcyI 233 GACGCC G[GA]^CG[CT]C 267 GGCGCC Alw44I 261 GTGCAC G^TGCAC 2318 GTGCAC ApaLI 261 GTGCAC G^TGCAC 2318 GTGCAC AseI 1867 ATTAAT AT^TAAT 1953 ATTAAT AsnI 1867 ATTAAT AT^TAAT 1953 ATTAAT AspI 540 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 3354 GACATAGTC BalI 22 TGGCCA TGG^CCA 809 TGGCCA BglI 700 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 811 GCCAGTTCGGC BsaAI 912 CACGTA [CT]AC^GT[GA] 1320 CACGTG BsaHI 233 GACGCC G[GA]^CG[CT]C 267 GGCGCC BsaI 994 GAGACC GGTCTC(1/5) 372 GGTCTC BsaWI 369 TCCGGT [AT]^CCGG[AT] 929 TCCGGA BsmI 2746 GCATTC GAATGC(1/-1) 4634 GCATTC BsrBI 272 CCGCTC CCGCTC(-3/-3) 355 CCGCTC BsrFI 409 GCCGGC [GA]^CCGG[CT] 864 GCCGGC Cfr10I 409 GCCGGC [GA]^CCGG[CT] 864 GCCGGC EcoT22I 2094 ATGCAT ATGCA^T 4092 ATGCAT Hin1I 233 GACGCC G[GA]^CG[CT]C 267 GGCGCC MluNI 22 TGGCCA TGG^CCA 809 TGGCCA MscI 22 TGGCCA TGG^CCA 809 TGGCCA NaeI 409 GCCGGC GCC^GGC 864 GCCGGC NcoI 47 CCATGG C^CATGG 2551 CCATGG NgoMIV 409 GCCGGC G^CCGGC 864 GCCGGC NsiI 2094 ATGCAT ATGCA^T 4092 ATGCAT PflFI 540 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 3354 GACATAGTC PshBI 1867 ATTAAT AT^TAAT 1953 ATTAAT Psp1406I 1336 AACGTT AA^CGTT 2415 AACGTT StuI 4646 AGGCCT AGG^CCT 4873 AGGCCT Tth111I 540 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 3354 GACATAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaBI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI