##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI AocI Aor51HI AscI AseI
AsnI AspEI AviII BbeI BcgI
BsaHI BseAI BsiEI BsiWI BspDI
BspEI BspMI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI FspI
HaeII HgaI Hin1I KasI MluI
MroI NarI NdeI NheI NotI
NruI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SbfI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 438 GGTACC G^GTACC
AccII 2101 CGCG CG^CG
AflII 957 CTTAAG C^TTAAG
Alw44I 3397 GTGCAC G^TGCAC
ApaLI 3397 GTGCAC G^TGCAC
Asp718I 438 GGTACC G^GTACC
AspI 4200 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2855 CCTAGG C^CTAGG
BalI 644 TGGCCA TGG^CCA
BciVI 2422 GGATAC GTATCC(6/5)
BclI 75 TGATCA T^GATCA
BfrI 957 CTTAAG C^TTAAG
BlnI 2855 CCTAGG C^CTAGG
BlpI 5066 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5066 GCTGAGC GC^T[ACGT]AGC
BsaI 2838 GAGACC GGTCTC(1/5)
BsaWI 2335 TCCGGT [AT]^CCGG[AT]
BsmBI 655 CGTCTC CGTCTC(1/5)
BspHI 4373 TCATGA T^CATGA
BspLU11I 5016 ACATGT A^CATGT
BsrFI 3671 GCCGGC [GA]^CCGG[CT]
BstEII 2188 GGTGACC G^GT[ACGT]ACC
BstPI 2188 GGTGACC G^GT[ACGT]ACC
BstUI 2101 CGCG CG^CG
CelII 5066 GCTGAGC GC^T[ACGT]AGC
CfoI 2139 GCGC GCG^C
Cfr10I 3671 GCCGGC [GA]^CCGG[CT]
EcoO65I 2188 GGTGACC G^GT[ACGT]ACC
EcoRV 850 GATATC GAT^ATC
EcoT22I 573 ATGCAT ATGCA^T
FbaI 75 TGATCA T^GATCA
FseI 3670 GGCCGGCC GGCCGG^CC
HhaI 2139 GCGC GCG^C
HinP1I 2139 GCGC G^CGC
HindIII 4869 AAGCTT A^AGCTT
KpnI 438 GGTACC GGTAC^C
KspI 2100 CCGCGG CCGC^GG
MluNI 644 TGGCCA TGG^CCA
MscI 644 TGGCCA TGG^CCA
MvnI 2101 CGCG CG^CG
NaeI 3671 GCCGGC GCC^GGC
NgoMIV 3671 GCCGGC G^CCGGC
NsiI 573 ATGCAT ATGCA^T
PflFI 4200 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1654 GACGGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4373 TCATGA T^CATGA
SacII 2100 CCGCGG CCGC^GG
ScaI 4893 AGTACT AGT^ACT
SfiI 3665 GGCCTGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 726 ACTAGT A^CTAGT
Tth111I 4200 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2834 TCTAGA T^CTAGA
XcmI 1262 CCATCATTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 1401 ACACGT A^C[GA][CT]GT
5016 ACATGT
ApaI 750 GGGCCC GGGCC^C
2155 GGGCCC
BanI 438 GGTACC G^G[CT][GA]CC
3457 GGTGCC
BbrPI 1402 CACGTG CAC^GTG
3462 CACGTG
BbvCI 3752 CCTCAGC CCTCAGC(-5/-2)
4492 CCTCAGC
BcnI 1644 CCGGG CC^[GC]GG
2685 CCGGG
BsaBI 1088 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4936 GATACAAATC
BsgI 880 GTGCAG GTGCAG(16/14)
935 GTGCAG
Bsp1407I 2587 TGTACA T^GTACA
5108 TGTACA
BsrBI 335 CCGCTC CCGCTC(-3/-3)
2842 CCGCTC
BsrGI 2587 TGTACA T^GTACA
5108 TGTACA
DraIII 356 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3790 CACTTTGTG
HpaI 2765 GTTAAC GTT^AAC
4957 GTTAAC
MamI 1088 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4936 GATACAAATC
NciI 1644 CCGGG CC^[GC]GG
2685 CCGGG
NcoI 251 CCATGG C^CATGG
1874 CCATGG
PaeR7I 2078 CTCGAG C^TCGAG
2240 CTCGAG
PflMI 2296 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2319 CCACGGTTTGG
PmaCI 1402 CACGTG CAC^GTG
3462 CACGTG
PmlI 1402 CACGTG CAC^GTG
3462 CACGTG
SalI 1729 GTCGAC G^TCGAC
2703 GTCGAC
SexAI 357 ACCAGGT A^CC[AT]GGT
1519 ACCAGGT
SspBI 2587 TGTACA T^GTACA
5108 TGTACA
Van91I 2296 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2319 CCACGGTTTGG
XhoI 2078 CTCGAG C^TCGAG
2240 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BamHI BanII BbsI
BbvI BfaI BglI BglII BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI