################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII AccI AclI AcyI AgeI AhdI AscI AseI AsnI AspEI BbeI BclI BsaBI BsaHI BsiWI BsmBI Bsp1407I BspDI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I FbaI Hin1I HpaI KasI KspI MamI MfeI MluI MunI NarI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 3477 GGTACC G^GTACC AccII 3384 CGCG CG^CG AccIII 1298 TCCGGA T^CCGGA AflII 1824 CTTAAG C^TTAAG Aor51HI 262 AGCGCT AGC^GCT Asp718I 3477 GGTACC G^GTACC AspI 3510 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1752 CCTAGG C^CTAGG BalI 3329 TGGCCA TGG^CCA BbvCI 4309 GCTGAGG CCTCAGC(-5/-2) BcgI 337 GCATCTATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1824 CTTAAG C^TTAAG BglII 908 AGATCT A^GATCT BlnI 1752 CCTAGG C^CTAGG BseAI 1298 TCCGGA T^CCGGA BsiEI 1214 CGGTCG CG[GA][CT]^CG BspEI 1298 TCCGGA T^CCGGA BspHI 2488 TCATGA T^CATGA BspLU11I 2621 ACATGT A^CATGT BsrBI 2085 CCGCTC CCGCTC(-3/-3) BstUI 3384 CGCG CG^CG DraI 1458 TTTAAA TTT^AAA DraIII 2498 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 262 AGCGCT AGC^GCT EcoRV 1655 GATATC GAT^ATC EcoT22I 623 ATGCAT ATGCA^T FseI 2863 GGCCGGCC GGCCGG^CC HgaI 4300 GCGTC GACGC(5/10) HindIII 611 AAGCTT A^AGCTT KpnI 3477 GGTACC GGTAC^C MluNI 3329 TGGCCA TGG^CCA MroI 1298 TCCGGA T^CCGGA MscI 3329 TGGCCA TGG^CCA MvnI 3384 CGCG CG^CG NaeI 2864 GCCGGC GCC^GGC NcoI 2969 CCATGG C^CATGG NdeI 620 CATATG CA^TATG NgoMIV 2864 GCCGGC G^CCGGC NsiI 623 ATGCAT ATGCA^T PflFI 3510 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 2488 TCATGA T^CATGA SacI 3225 GAGCTC GAGCT^C SmaI 1306 CCCGGG CCC^GGG Tth111I 3510 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1306 CCCGGG C^CCGGG XmaI 1306 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Alw44I 2664 GTGCAC G^TGCAC 3677 GTGCAC AocI 1744 CCTCAGG CC^T[ACGT]AGG 3295 CCTCAGG ApaI 761 GGGCCC GGGCC^C 769 GGGCCC ApaLI 2664 GTGCAC G^TGCAC 3677 GTGCAC Asp700I 2276 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3903 GAAAGGTTTC AvaI 113 CTCGGG C^[CT]CG[GA]G 1306 CCCGGG BamHI 829 GGATCC G^GATCC 4249 GGATCC BbrPI 807 CACGTG CAC^GTG 4118 CACGTG BciVI 3352 GTATCC GTATCC(6/5) 3559 GTATCC BglI 576 GCCACTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2872 GCCTCATAGGC BsaAI 807 CACGTG [CT]AC^GT[GA] 4118 CACGTG BsaWI 292 TCCGGT [AT]^CCGG[AT] 1298 TCCGGA BsmI 814 GCATTC GAATGC(1/-1) 1086 GCATTC BsoBI 113 CTCGGG C^[CT]CG[GA]G 1306 CCCGGG BstAPI 1311 GCAGACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3178 GCAAGGAGTGC Bsu36I 1744 CCTCAGG CC^T[ACGT]AGG 3295 CCTCAGG DrdI 530 GACACCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3726 GACATCCGAGTC DsaI 2969 CCATGG C^C[GA][CT]GG 3449 CCACGG Eco81I 1744 CCTCAGG CC^T[ACGT]AGG 3295 CCTCAGG PflMI 205 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2213 CCAGGAGCTGG PmaCI 807 CACGTG CAC^GTG 4118 CACGTG PmlI 807 CACGTG CAC^GTG 4118 CACGTG SphI 1025 GCATGC GCATG^C 1181 GCATGC StuI 250 AGGCCT AGG^CCT 1193 AGGCCT Tsp45I 3214 GTGAC ^GT[GC]AC 4116 GTCAC Van91I 205 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2213 CCAGGAGCTGG XmnI 2276 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3903 GAAAGGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII AviII BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII