##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccI AclI AcyI AgeI
AhdI AscI AseI AsnI AspEI
BbeI BclI BsaBI BsaHI BsiWI
BsmBI Bsp1407I BspDI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoO65I FbaI
Hin1I HpaI KasI KspI MamI
MfeI MluI MunI NarI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3477 GGTACC G^GTACC
AccII 3384 CGCG CG^CG
AccIII 1298 TCCGGA T^CCGGA
AflII 1824 CTTAAG C^TTAAG
Aor51HI 262 AGCGCT AGC^GCT
Asp718I 3477 GGTACC G^GTACC
AspI 3510 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1752 CCTAGG C^CTAGG
BalI 3329 TGGCCA TGG^CCA
BbvCI 4309 GCTGAGG CCTCAGC(-5/-2)
BcgI 337 GCATCTATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1824 CTTAAG C^TTAAG
BglII 908 AGATCT A^GATCT
BlnI 1752 CCTAGG C^CTAGG
BseAI 1298 TCCGGA T^CCGGA
BsiEI 1214 CGGTCG CG[GA][CT]^CG
BspEI 1298 TCCGGA T^CCGGA
BspHI 2488 TCATGA T^CATGA
BspLU11I 2621 ACATGT A^CATGT
BsrBI 2085 CCGCTC CCGCTC(-3/-3)
BstUI 3384 CGCG CG^CG
DraI 1458 TTTAAA TTT^AAA
DraIII 2498 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 262 AGCGCT AGC^GCT
EcoRV 1655 GATATC GAT^ATC
EcoT22I 623 ATGCAT ATGCA^T
FseI 2863 GGCCGGCC GGCCGG^CC
HgaI 4300 GCGTC GACGC(5/10)
HindIII 611 AAGCTT A^AGCTT
KpnI 3477 GGTACC GGTAC^C
MluNI 3329 TGGCCA TGG^CCA
MroI 1298 TCCGGA T^CCGGA
MscI 3329 TGGCCA TGG^CCA
MvnI 3384 CGCG CG^CG
NaeI 2864 GCCGGC GCC^GGC
NcoI 2969 CCATGG C^CATGG
NdeI 620 CATATG CA^TATG
NgoMIV 2864 GCCGGC G^CCGGC
NsiI 623 ATGCAT ATGCA^T
PflFI 3510 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2488 TCATGA T^CATGA
SacI 3225 GAGCTC GAGCT^C
SmaI 1306 CCCGGG CCC^GGG
Tth111I 3510 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1306 CCCGGG C^CCGGG
XmaI 1306 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Alw44I 2664 GTGCAC G^TGCAC
3677 GTGCAC
AocI 1744 CCTCAGG CC^T[ACGT]AGG
3295 CCTCAGG
ApaI 761 GGGCCC GGGCC^C
769 GGGCCC
ApaLI 2664 GTGCAC G^TGCAC
3677 GTGCAC
Asp700I 2276 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3903 GAAAGGTTTC
AvaI 113 CTCGGG C^[CT]CG[GA]G
1306 CCCGGG
BamHI 829 GGATCC G^GATCC
4249 GGATCC
BbrPI 807 CACGTG CAC^GTG
4118 CACGTG
BciVI 3352 GTATCC GTATCC(6/5)
3559 GTATCC
BglI 576 GCCACTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2872 GCCTCATAGGC
BsaAI 807 CACGTG [CT]AC^GT[GA]
4118 CACGTG
BsaWI 292 TCCGGT [AT]^CCGG[AT]
1298 TCCGGA
BsmI 814 GCATTC GAATGC(1/-1)
1086 GCATTC
BsoBI 113 CTCGGG C^[CT]CG[GA]G
1306 CCCGGG
BstAPI 1311 GCAGACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3178 GCAAGGAGTGC
Bsu36I 1744 CCTCAGG CC^T[ACGT]AGG
3295 CCTCAGG
DrdI 530 GACACCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3726 GACATCCGAGTC
DsaI 2969 CCATGG C^C[GA][CT]GG
3449 CCACGG
Eco81I 1744 CCTCAGG CC^T[ACGT]AGG
3295 CCTCAGG
PflMI 205 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2213 CCAGGAGCTGG
PmaCI 807 CACGTG CAC^GTG
4118 CACGTG
PmlI 807 CACGTG CAC^GTG
4118 CACGTG
SphI 1025 GCATGC GCATG^C
1181 GCATGC
StuI 250 AGGCCT AGG^CCT
1193 AGGCCT
Tsp45I 3214 GTGAC ^GT[GC]AC
4116 GTCAC
Van91I 205 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2213 CCAGGAGCTGG
XmnI 2276 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3903 GAAAGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
AviII BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII