################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AatII Acc65I AccIII AclI AscI Asp718I BamHI BbeI BsaBI BseAI BsiWI BsmBI BspDI BspEI Bst1107I BstZ17I ClaI CpoI EcoT22I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NotI NruI NsiI PacI PaeR7I PmeI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfoI SgrAI SnaBI SphI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 1021 GTCTAC GT^[AC][GT]AC AcyI 2946 GGCGTC G[GA]^CG[CT]C AgeI 290 ACCGGT A^CCGGT ApaI 4793 GGGCCC GGGCC^C AspI 1549 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 6498 CCTAGG C^CTAGG BlnI 6498 CCTAGG C^CTAGG BlpI 2406 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2406 GCTCAGC GC^T[ACGT]AGC BsaHI 2946 GGCGTC G[GA]^CG[CT]C BsaWI 290 ACCGGT [AT]^CCGG[AT] BspLU11I 3121 ACATGT A^CATGT BsrBI 4822 GAGCGG CCGCTC(-3/-3) BssHII 25 GCGCGC G^CGCGC BstBI 5515 TTCGAA TT^CGAA CelII 2406 GCTCAGC GC^T[ACGT]AGC EagI 4539 CGGCCG C^GGCCG EclXI 4539 CGGCCG C^GGCCG Eco52I 4539 CGGCCG C^GGCCG EcoRI 697 GAATTC G^AATTC FseI 3412 GGCCGGCC GGCCGG^CC Hin1I 2946 GGCGTC G[GA]^CG[CT]C NheI 873 GCTAGC G^CTAGC NspV 5515 TTCGAA TT^CGAA PflFI 1549 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 290 ACCGGT A^CCGGT PshAI 4218 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 253 GAGCTC GAGCT^C SapI 5388 GAAGAGC GCTCTTC(1/4) SfiI 39 GGCCTCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 5515 TTCGAA TT^CGAA Tth111I 1549 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 5936 CCAGCCTAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflIII 3121 ACATGT A^C[GA][CT]GT 3147 ACACGT AhdI 286 GACTACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1975 GACAAGCTGTC Alw44I 763 GTGCAC G^TGCAC 2962 GTGCAC ApaLI 763 GTGCAC G^TGCAC 2962 GTGCAC AspEI 286 GACTACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1975 GACAAGCTGTC AviII 449 TGCGCA TGC^GCA 4828 TGCGCA BbvCI 4643 GCTGAGG CCTCAGC(-5/-2) 5022 GCTGAGG BciVI 626 GTATCC GTATCC(6/5) 1563 GTATCC BclI 3246 TGATCA T^GATCA 6759 TGATCA BglII 1776 AGATCT A^GATCT 2548 AGATCT BsiEI 1961 CGGTCG CG[GA][CT]^CG 4539 CGGCCG BsmI 2058 GAATGC GAATGC(1/-1) 5744 GCATTC Bsp1407I 2797 TGTACA T^GTACA 6052 TGTACA BsrDI 879 CATTGC GCAATG(2/0) 4177 CATTGC BsrGI 2797 TGTACA T^GTACA 6052 TGTACA BssSI 51 CACGAG CACGAG(-5/-1) 3055 CTCGTG DraI 4922 TTTAAA TTT^AAA 6912 TTTAAA DrdI 202 GACCGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4515 GACTAGTCGGTC Eam1105I 286 GACTACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1975 GACAAGCTGTC EcoRV 1636 GATATC GAT^ATC 2457 GATATC FbaI 3246 TGATCA T^GATCA 6759 TGATCA FspI 449 TGCGCA TGC^GCA 4828 TGCGCA PflMI 2334 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4981 CCAGAGTTTGG PpuMI 232 GGGACCC [GA]G^G[AT]CC[CT] 5279 AGGTCCC SpeI 1326 ACTAGT A^CTAGT 4516 ACTAGT SspBI 2797 TGTACA T^GTACA 6052 TGTACA Van91I 2334 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4981 CCAGAGTTTGG XbaI 3503 TCTAGA T^CTAGA 3900 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 146 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AluI AlwI AlwNI AocI Aor51HI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BcgI BcnI BfaI BfrI BglI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeI NdeII NgoMIV NlaIII NlaIV NspI PleI PmaCI PmlI PshBI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI XmnI