##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII Acc65I AccIII AclI AscI
Asp718I BamHI BbeI BsaBI BseAI
BsiWI BsmBI BspDI BspEI Bst1107I
BstZ17I ClaI CpoI EcoT22I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NarI
NotI NruI NsiI PacI PaeR7I
PmeI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfoI SgrAI
SnaBI SphI Sse8387I SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1021 GTCTAC GT^[AC][GT]AC
AcyI 2946 GGCGTC G[GA]^CG[CT]C
AgeI 290 ACCGGT A^CCGGT
ApaI 4793 GGGCCC GGGCC^C
AspI 1549 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6498 CCTAGG C^CTAGG
BlnI 6498 CCTAGG C^CTAGG
BlpI 2406 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2406 GCTCAGC GC^T[ACGT]AGC
BsaHI 2946 GGCGTC G[GA]^CG[CT]C
BsaWI 290 ACCGGT [AT]^CCGG[AT]
BspLU11I 3121 ACATGT A^CATGT
BsrBI 4822 GAGCGG CCGCTC(-3/-3)
BssHII 25 GCGCGC G^CGCGC
BstBI 5515 TTCGAA TT^CGAA
CelII 2406 GCTCAGC GC^T[ACGT]AGC
EagI 4539 CGGCCG C^GGCCG
EclXI 4539 CGGCCG C^GGCCG
Eco52I 4539 CGGCCG C^GGCCG
EcoRI 697 GAATTC G^AATTC
FseI 3412 GGCCGGCC GGCCGG^CC
Hin1I 2946 GGCGTC G[GA]^CG[CT]C
NheI 873 GCTAGC G^CTAGC
NspV 5515 TTCGAA TT^CGAA
PflFI 1549 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 290 ACCGGT A^CCGGT
PshAI 4218 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 253 GAGCTC GAGCT^C
SapI 5388 GAAGAGC GCTCTTC(1/4)
SfiI 39 GGCCTCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5515 TTCGAA TT^CGAA
Tth111I 1549 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 5936 CCAGCCTAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 3121 ACATGT A^C[GA][CT]GT
3147 ACACGT
AhdI 286 GACTACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1975 GACAAGCTGTC
Alw44I 763 GTGCAC G^TGCAC
2962 GTGCAC
ApaLI 763 GTGCAC G^TGCAC
2962 GTGCAC
AspEI 286 GACTACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1975 GACAAGCTGTC
AviII 449 TGCGCA TGC^GCA
4828 TGCGCA
BbvCI 4643 GCTGAGG CCTCAGC(-5/-2)
5022 GCTGAGG
BciVI 626 GTATCC GTATCC(6/5)
1563 GTATCC
BclI 3246 TGATCA T^GATCA
6759 TGATCA
BglII 1776 AGATCT A^GATCT
2548 AGATCT
BsiEI 1961 CGGTCG CG[GA][CT]^CG
4539 CGGCCG
BsmI 2058 GAATGC GAATGC(1/-1)
5744 GCATTC
Bsp1407I 2797 TGTACA T^GTACA
6052 TGTACA
BsrDI 879 CATTGC GCAATG(2/0)
4177 CATTGC
BsrGI 2797 TGTACA T^GTACA
6052 TGTACA
BssSI 51 CACGAG CACGAG(-5/-1)
3055 CTCGTG
DraI 4922 TTTAAA TTT^AAA
6912 TTTAAA
DrdI 202 GACCGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4515 GACTAGTCGGTC
Eam1105I 286 GACTACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1975 GACAAGCTGTC
EcoRV 1636 GATATC GAT^ATC
2457 GATATC
FbaI 3246 TGATCA T^GATCA
6759 TGATCA
FspI 449 TGCGCA TGC^GCA
4828 TGCGCA
PflMI 2334 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4981 CCAGAGTTTGG
PpuMI 232 GGGACCC [GA]G^G[AT]CC[CT]
5279 AGGTCCC
SpeI 1326 ACTAGT A^CTAGT
4516 ACTAGT
SspBI 2797 TGTACA T^GTACA
6052 TGTACA
Van91I 2334 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4981 CCAGAGTTTGG
XbaI 3503 TCTAGA T^CTAGA
3900 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI AlwNI AocI Aor51HI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvI BcgI BcnI
BfaI BfrI BglI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeI NdeII NgoMIV NlaIII NlaIV
NspI PleI PmaCI PmlI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI
XmnI