##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AclI AcsI AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I AspEI BbeI BclI
BseAI BsiWI BspDI BspEI BspHI
Bst1107I BstBI BstZ17I ClaI CpoI
Eam1105I Eco47III EcoRI EcoRV FbaI
KasI MfeI MluI MroI MunI
NarI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 2402 GTAGAC GT^[AC][GT]AC
AcyI 1603 GACGCC G[GA]^CG[CT]C
AflII 3410 CTTAAG C^TTAAG
Alw44I 645 GTGCAC G^TGCAC
ApaLI 645 GTGCAC G^TGCAC
BalI 4422 TGGCCA TGG^CCA
BciVI 2798 GTATCC GTATCC(6/5)
BfrI 3410 CTTAAG C^TTAAG
BglI 451 GCCGCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 605 AGATCT A^GATCT
BsaHI 1603 GACGCC G[GA]^CG[CT]C
BsaWI 3308 TCCGGT [AT]^CCGG[AT]
BsiEI 449 CGGCCG CG[GA][CT]^CG
BsmI 1579 GCATTC GAATGC(1/-1)
BsrBI 129 GAGCGG CCGCTC(-3/-3)
BsrDI 3862 CATTGC GCAATG(2/0)
BssHII 482 GCGCGC G^CGCGC
BssSI 4502 CTCGTG CACGAG(-5/-1)
BstEII 803 GGTGACC G^GT[ACGT]ACC
BstPI 803 GGTGACC G^GT[ACGT]ACC
BstXI 1167 CCACCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 3825 TTTAAA TTT^AAA
DrdI 4920 GACATCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 449 CGGCCG C^GGCCG
EclXI 449 CGGCCG C^GGCCG
Eco52I 449 CGGCCG C^GGCCG
EcoO65I 803 GGTGACC G^GT[ACGT]ACC
EcoT22I 1239 ATGCAT ATGCA^T
FseI 4134 GGCCGGCC GGCCGG^CC
HaeII 530 GGCGCT [GA]GCGC^[CT]
Hin1I 1603 GACGCC G[GA]^CG[CT]C
HincII 2811 GTTAAC GT[CT]^[GA]AC
HindII 2811 GTTAAC GT[CT]^[GA]AC
HindIII 755 AAGCTT A^AGCTT
HpaI 2811 GTTAAC GTT^AAC
KspI 4840 CCGCGG CCGC^GG
MluNI 4422 TGGCCA TGG^CCA
MscI 4422 TGGCCA TGG^CCA
NdeI 1261 CATATG CA^TATG
NheI 548 GCTAGC G^CTAGC
NotI 448 GCGGCCGC GC^GGCCGC
NsiI 1239 ATGCAT ATGCA^T
PshAI 2583 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 4840 CCGCGG CCGC^GG
SbfI 1079 CCTGCAGG CCTGCA^GG
SexAI 1823 ACCTGGT A^CC[AT]GGT
SmaI 2836 CCCGGG CCC^GGG
SphI 2356 GCATGC GCATG^C
Sse8387I 1079 CCTGCAGG CCTGCA^GG
XbaI 3392 TCTAGA T^CTAGA
XmaCI 2836 CCCGGG C^CCGGG
XmaI 2836 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Acc65I 3058 GGTACC G^GTACC
4681 GGTACC
Asp718I 3058 GGTACC G^GTACC
4681 GGTACC
AviII 778 TGCGCA TGC^GCA
1576 TGCGCA
BbvCI 2540 CCTCAGC CCTCAGC(-5/-2)
2595 CCTCAGC
BcgI 105 GCAAGAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2624 GCAGGGCCCTCG
Bsp1407I 2287 TGTACA T^GTACA
4270 TGTACA
BspLU11I 2020 ACATGT A^CATGT
4627 ACATGT
BsrGI 2287 TGTACA T^GTACA
4270 TGTACA
BstAPI 3232 GCATCGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5007 GCACTGACTGC
FspI 778 TGCGCA TGC^GCA
1576 TGCGCA
KpnI 3058 GGTACC GGTAC^C
4681 GGTACC
NaeI 3228 GCCGGC GCC^GGC
4135 GCCGGC
NcoI 1706 CCATGG C^CATGG
4892 CCATGG
NgoMIV 3228 GCCGGC G^CCGGC
4135 GCCGGC
SapI 858 GCTCTTC GCTCTTC(1/4)
5016 GCTCTTC
SspBI 2287 TGTACA T^GTACA
4270 TGTACA
Tsp509I 1689 AATT ^AATT
3841 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AspI AvaI AvaII AvrII BamHI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
Tth111I TthHB8I Van91I XcmI XhoII