##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AcyI AflII AflIII
AgeI AhdI Aor51HI AscI Asp700I
Asp718I AspEI BbeI BbrPI BcgI
BciVI BclI BfrI BsaAI BsaHI
BsiEI BsiWI BsmBI BspLU11I BssHII
BstBI BstEII BstPI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI HgaI Hin1I HpaI KasI
KpnI KspI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PinAI PmaCI PmeI PmlI
PvuI RsrII SacII SalI SapI
SexAI SfoI SfuI SgrAI SnaBI
SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 2977 CGCG CG^CG
AccIII 574 TCCGGA T^CCGGA
AclI 67 AACGTT AA^CGTT
AocI 3856 CCTCAGG CC^T[ACGT]AGG
ApaI 1513 GGGCCC GGGCC^C
AseI 1692 ATTAAT AT^TAAT
AsnI 1692 ATTAAT AT^TAAT
AviII 2608 TGCGCA TGC^GCA
BlpI 3089 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3089 GCTGAGC GC^T[ACGT]AGC
BsaBI 2174 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 574 TCCGGA [AT]^CCGG[AT]
BseAI 574 TCCGGA T^CCGGA
BspDI 627 ATCGAT AT^CGAT
BspEI 574 TCCGGA T^CCGGA
BsrDI 1166 CATTGC GCAATG(2/0)
BsrFI 4286 GCCGGT [GA]^CCGG[CT]
BssSI 2122 CTCGTG CACGAG(-5/-1)
Bst1107I 2532 GTATAC GTA^TAC
BstAPI 1280 GCAGCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2977 CGCG CG^CG
BstZ17I 2532 GTATAC GTA^TAC
Bsu36I 3856 CCTCAGG CC^T[ACGT]AGG
CelII 3089 GCTGAGC GC^T[ACGT]AGC
Cfr10I 4286 GCCGGT [GA]^CCGG[CT]
ClaI 627 ATCGAT AT^CGAT
Eco81I 3856 CCTCAGG CC^T[ACGT]AGG
FspI 2608 TGCGCA TGC^GCA
HaeII 253 GGCGCT [GA]GCGC^[CT]
HindIII 4767 AAGCTT A^AGCTT
MaeII 68 ACGT A^CGT
MamI 2174 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2652 CAATTG C^AATTG
MroI 574 TCCGGA T^CCGGA
MunI 2652 CAATTG C^AATTG
MvnI 2977 CGCG CG^CG
PacI 1689 TTAATTAA TTAAT^TAA
PaeR7I 1006 CTCGAG C^TCGAG
PshAI 4722 GACTAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1692 ATTAAT AT^TAAT
Psp1406I 67 AACGTT AA^CGTT
ScaI 4317 AGTACT AGT^ACT
SfiI 3561 GGCCTCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1273 CCCGGG CCC^GGG
SpeI 87 ACTAGT A^CTAGT
XbaI 4919 TCTAGA T^CTAGA
XhoI 1006 CTCGAG C^TCGAG
XmaCI 1273 CCCGGG C^CCGGG
XmaI 1273 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 2532 GTATAC GT^[AC][GT]AC
4643 GTAGAC
AspI 903 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3337 GACTGTGTC
BanI 1878 GGCACC G^G[CT][GA]CC
2028 GGCACC
Bsp1407I 411 TGTACA T^GTACA
5065 TGTACA
BspHI 520 TCATGA T^CATGA
1753 TCATGA
BsrBI 3148 CCGCTC CCGCTC(-3/-3)
3015 GAGCGG
BsrGI 411 TGTACA T^GTACA
5065 TGTACA
DraI 2844 TTTAAA TTT^AAA
3681 TTTAAA
EcoRV 129 GATATC GAT^ATC
1790 GATATC
FauI 2347 CCCGC CCCGC(4/6)
592 GCGGG
PflFI 903 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3337 GACTGTGTC
RcaI 520 TCATGA T^CATGA
1753 TCATGA
SbfI 242 CCTGCAGG CCTGCA^GG
4980 CCTGCAGG
SphI 1242 GCATGC GCATG^C
2104 GCATGC
Sse8387I 242 CCTGCAGG CCTGCA^GG
4980 CCTGCAGG
SspBI 411 TGTACA T^GTACA
5065 TGTACA
Tth111I 903 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3337 GACTGTGTC
XcmI 3383 CCACAGGGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3412 CCACCCAGCACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AvaI AvaII AvrII BalI BamHI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BlnI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII