################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AcyI AflII AflIII AgeI AhdI Aor51HI AscI Asp700I Asp718I AspEI BbeI BbrPI BcgI BciVI BclI BfrI BsaAI BsaHI BsiEI BsiWI BsmBI BspLU11I BssHII BstBI BstEII BstPI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI HgaI Hin1I HpaI KasI KpnI KspI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SapI SexAI SfoI SfuI SgrAI SnaBI SspI SwaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 2977 CGCG CG^CG AccIII 574 TCCGGA T^CCGGA AclI 67 AACGTT AA^CGTT AocI 3856 CCTCAGG CC^T[ACGT]AGG ApaI 1513 GGGCCC GGGCC^C AseI 1692 ATTAAT AT^TAAT AsnI 1692 ATTAAT AT^TAAT AviII 2608 TGCGCA TGC^GCA BlpI 3089 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3089 GCTGAGC GC^T[ACGT]AGC BsaBI 2174 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 574 TCCGGA [AT]^CCGG[AT] BseAI 574 TCCGGA T^CCGGA BspDI 627 ATCGAT AT^CGAT BspEI 574 TCCGGA T^CCGGA BsrDI 1166 CATTGC GCAATG(2/0) BsrFI 4286 GCCGGT [GA]^CCGG[CT] BssSI 2122 CTCGTG CACGAG(-5/-1) Bst1107I 2532 GTATAC GTA^TAC BstAPI 1280 GCAGCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 2977 CGCG CG^CG BstZ17I 2532 GTATAC GTA^TAC Bsu36I 3856 CCTCAGG CC^T[ACGT]AGG CelII 3089 GCTGAGC GC^T[ACGT]AGC Cfr10I 4286 GCCGGT [GA]^CCGG[CT] ClaI 627 ATCGAT AT^CGAT Eco81I 3856 CCTCAGG CC^T[ACGT]AGG FspI 2608 TGCGCA TGC^GCA HaeII 253 GGCGCT [GA]GCGC^[CT] HindIII 4767 AAGCTT A^AGCTT MaeII 68 ACGT A^CGT MamI 2174 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2652 CAATTG C^AATTG MroI 574 TCCGGA T^CCGGA MunI 2652 CAATTG C^AATTG MvnI 2977 CGCG CG^CG PacI 1689 TTAATTAA TTAAT^TAA PaeR7I 1006 CTCGAG C^TCGAG PshAI 4722 GACTAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1692 ATTAAT AT^TAAT Psp1406I 67 AACGTT AA^CGTT ScaI 4317 AGTACT AGT^ACT SfiI 3561 GGCCTCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1273 CCCGGG CCC^GGG SpeI 87 ACTAGT A^CTAGT XbaI 4919 TCTAGA T^CTAGA XhoI 1006 CTCGAG C^TCGAG XmaCI 1273 CCCGGG C^CCGGG XmaI 1273 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 2532 GTATAC GT^[AC][GT]AC 4643 GTAGAC AspI 903 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3337 GACTGTGTC BanI 1878 GGCACC G^G[CT][GA]CC 2028 GGCACC Bsp1407I 411 TGTACA T^GTACA 5065 TGTACA BspHI 520 TCATGA T^CATGA 1753 TCATGA BsrBI 3148 CCGCTC CCGCTC(-3/-3) 3015 GAGCGG BsrGI 411 TGTACA T^GTACA 5065 TGTACA DraI 2844 TTTAAA TTT^AAA 3681 TTTAAA EcoRV 129 GATATC GAT^ATC 1790 GATATC FauI 2347 CCCGC CCCGC(4/6) 592 GCGGG PflFI 903 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3337 GACTGTGTC RcaI 520 TCATGA T^CATGA 1753 TCATGA SbfI 242 CCTGCAGG CCTGCA^GG 4980 CCTGCAGG SphI 1242 GCATGC GCATG^C 2104 GCATGC Sse8387I 242 CCTGCAGG CCTGCA^GG 4980 CCTGCAGG SspBI 411 TGTACA T^GTACA 5065 TGTACA Tth111I 903 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3337 GACTGTGTC XcmI 3383 CCACAGGGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3412 CCACCCAGCACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaI AvaII AvrII BalI BamHI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII