##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AgeI ApoI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BbrPI BcgI BclI BlnI BsaBI
BsiEI BsiWI Bsp1407I BspDI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoRI EcoRV FbaI FseI FspI
HpaI KpnI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SfuI SgrAI SmaI
SnaBI SspBI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccII 1842 CGCG CG^CG
AhdI 3752 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3643 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3752 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2334 CTCGGG C^[CT]CG[GA]G
BbeI 716 GGCGCC GGCGC^C
BglII 1481 AGATCT A^GATCT
BsoBI 2334 CTCGGG C^[CT]CG[GA]G
BspLU11I 5595 ACATGT A^CATGT
BsrBI 154 GAGCGG CCGCTC(-3/-3)
BstUI 1842 CGCG CG^CG
DraI 3670 TTTAAA TTT^AAA
DrdI 1489 GACAAACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3752 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 179 ATGCAT ATGCA^T
FauI 1840 CCCGC CCCGC(4/6)
HincII 1115 GTCAAC GT[CT]^[GA]AC
HindII 1115 GTCAAC GT[CT]^[GA]AC
KasI 716 GGCGCC G^GCGCC
MvnI 1842 CGCG CG^CG
NaeI 1058 GCCGGC GCC^GGC
NarI 716 GGCGCC GG^CGCC
NgoMIV 1058 GCCGGC G^CCGGC
NsiI 179 ATGCAT ATGCA^T
SbfI 2161 CCTGCAGG CCTGCA^GG
ScaI 911 AGTACT AGT^ACT
SexAI 4059 ACCAGGT A^CC[AT]GGT
SfiI 3724 GGCCATTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 716 GGCGCC GGC^GCC
SpeI 1137 ACTAGT A^CTAGT
SphI 1913 GCATGC GCATG^C
Sse8387I 2161 CCTGCAGG CCTGCA^GG
SspI 805 AATATT AAT^ATT
XmnI 3643 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 716 GGCGCC G[GA]^CG[CT]C
1674 GACGCC
AflII 1897 CTTAAG C^TTAAG
3661 CTTAAG
AflIII 5325 ACGTGT A^C[GA][CT]GT
5595 ACATGT
Alw44I 577 GTGCAC G^TGCAC
2858 GTGCAC
Aor51HI 3845 AGCGCT AGC^GCT
4563 AGCGCT
ApaI 353 GGGCCC GGGCC^C
2831 GGGCCC
ApaLI 577 GTGCAC G^TGCAC
2858 GTGCAC
BbvCI 3710 CCTCAGC CCTCAGC(-5/-2)
4034 CCTCAGC
BciVI 4051 GGATAC GTATCC(6/5)
4546 GGATAC
BfrI 1897 CTTAAG C^TTAAG
3661 CTTAAG
BglI 3725 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3864 GCCTTCCTGGC
BsaAI 1774 CACGTA [CT]AC^GT[GA]
5324 TACGTG
BsaHI 716 GGCGCC G[GA]^CG[CT]C
1674 GACGCC
BsmBI 798 CGTCTC CGTCTC(1/5)
3444 GAGACG
BsmI 2501 GAATGC GAATGC(1/-1)
344 GCATTC
BsrFI 1058 GCCGGC [GA]^CCGG[CT]
1103 GCCGGT
BstEII 1704 GGTCACC G^GT[ACGT]ACC
4819 GGTGACC
BstPI 1704 GGTCACC G^GT[ACGT]ACC
4819 GGTGACC
Cfr10I 1058 GCCGGC [GA]^CCGG[CT]
1103 GCCGGT
Eco47III 3845 AGCGCT AGC^GCT
4563 AGCGCT
EcoO65I 1704 GGTCACC G^GT[ACGT]ACC
4819 GGTGACC
Hin1I 716 GGCGCC G[GA]^CG[CT]C
1674 GACGCC
PshAI 4407 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5424 GACCAGTGTC
SacI 762 GAGCTC GAGCT^C
5550 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspEI BspMI
BsrDI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII