##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
AscI AviII BamHI BbrPI BclI
BsaAI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrFI BssHII BstBI
BstEII BstPI Cfr10I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoO65I FbaI FseI FspI
HincII HindII HpaI MluI MroI
NaeI NgoMIV NotI NspV PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2276 GGTACC G^GTACC
AcyI 3771 GGCGCC G[GA]^CG[CT]C
AhdI 5109 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 5993 ATTAAT AT^TAAT
AsnI 5993 ATTAAT AT^TAAT
Asp700I 5535 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2276 GGTACC G^GTACC
AspEI 5109 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2324 CCTAGG C^CTAGG
BbeI 3771 GGCGCC GGCGC^C
BbvCI 3280 CCTCAGC CCTCAGC(-5/-2)
BglI 3774 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5070 AGATCT A^GATCT
BlnI 2324 CCTAGG C^CTAGG
BsaBI 147 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3771 GGCGCC G[GA]^CG[CT]C
BsrDI 3634 CATTGC GCAATG(2/0)
BssSI 2002 CACGAG CACGAG(-5/-1)
Bst1107I 4264 GTATAC GTA^TAC
BstZ17I 4264 GTATAC GTA^TAC
Eam1105I 5109 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 5322 GATATC GAT^ATC
Hin1I 3771 GGCGCC G[GA]^CG[CT]C
HindIII 5686 AAGCTT A^AGCTT
KasI 3771 GGCGCC G^GCGCC
KpnI 2276 GGTACC GGTAC^C
KspI 72 CCGCGG CCGC^GG
MamI 147 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5139 CAATTG C^AATTG
MunI 5139 CAATTG C^AATTG
NarI 3771 GGCGCC GG^CGCC
NheI 385 GCTAGC G^CTAGC
NruI 82 TCGCGA TCG^CGA
PaeR7I 254 CTCGAG C^TCGAG
PshAI 2133 GACTTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5993 ATTAAT AT^TAAT
PstI 1292 CTGCAG CTGCA^G
SacII 72 CCGCGG CCGC^GG
SfoI 3771 GGCGCC GGC^GCC
XhoI 254 CTCGAG C^TCGAG
XmnI 5535 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
ApaI 1613 GGGCCC GGGCC^C
1919 GGGCCC
BcgI 1654 CGACTGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2004 CGAGAAGTTTGC
BsaWI 138 ACCGGA [AT]^CCGG[AT]
3690 TCCGGT
Bsp1407I 3878 TGTACA T^GTACA
5369 TGTACA
BsrBI 61 CCGCTC CCGCTC(-3/-3)
38 GAGCGG
BsrGI 3878 TGTACA T^GTACA
5369 TGTACA
BstAPI 1631 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6240 GCATGTGGTGC
HaeII 1643 AGCGCC [GA]GCGC^[CT]
3771 GGCGCC
SapI 743 GAAGAGC GCTCTTC(1/4)
2090 GCTCTTC
ScaI 4520 AGTACT AGT^ACT
6182 AGTACT
SmaI 1916 CCCGGG CCC^GGG
3759 CCCGGG
SspBI 3878 TGTACA T^GTACA
5369 TGTACA
XbaI 5180 TCTAGA T^CTAGA
6308 TCTAGA
XcmI 333 CCATGCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1976 CCATGAACCAGATGG
XmaCI 1916 CCCGGG C^CCGGG
3759 CCCGGG
XmaI 1916 CCCGGG C^CCGGG
3759 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AflIII AluI Alw44I AlwI
AlwNI AocI ApaLI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BfrI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NsiI NspI
PflFI PflMI PleI PpuMI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XhoII