##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AflII AscI AseI AsnI
BbeI BfrI BsaBI BsiWI BspDI
BsrBI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco52I
EcoO65I EcoRV FseI HindIII HpaI
KasI MamI MfeI MluI MunI
NarI NheI NotI NruI NspV
PacI PmeI PshBI PvuI RsrII
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 5191 GGTACC G^GTACC
AccIII 79 TCCGGA T^CCGGA
AclI 5974 AACGTT AA^CGTT
AcyI 3298 GACGCC G[GA]^CG[CT]C
AgeI 3099 ACCGGT A^CCGGT
Alw44I 103 GTGCAC G^TGCAC
ApaLI 103 GTGCAC G^TGCAC
Asp718I 5191 GGTACC G^GTACC
BciVI 307 GGATAC GTATCC(6/5)
BglI 3459 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 3298 GACGCC G[GA]^CG[CT]C
BseAI 79 TCCGGA T^CCGGA
BsiEI 4012 CGACCG CG[GA][CT]^CG
BsmBI 5866 GAGACG CGTCTC(1/5)
BsmI 2229 GAATGC GAATGC(1/-1)
Bsp1407I 4113 TGTACA T^GTACA
BspEI 79 TCCGGA T^CCGGA
BsrGI 4113 TGTACA T^GTACA
BssSI 88 CACGAG CACGAG(-5/-1)
BstAPI 4283 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoRI 2882 GAATTC G^AATTC
Hin1I 3298 GACGCC G[GA]^CG[CT]C
KpnI 5191 GGTACC GGTAC^C
KspI 35 CCGCGG CCGC^GG
MroI 79 TCCGGA T^CCGGA
NaeI 4047 GCCGGC GCC^GGC
NgoMIV 4047 GCCGGC G^CCGGC
PaeR7I 1953 CTCGAG C^TCGAG
PflMI 595 CCATTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3099 ACCGGT A^CCGGT
PshAI 4511 GACGTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5974 AACGTT AA^CGTT
SacII 35 CCGCGG CCGC^GG
SalI 1270 GTCGAC G^TCGAC
ScaI 4338 AGTACT AGT^ACT
SspBI 4113 TGTACA T^GTACA
SspI 6072 AATATT AAT^ATT
StuI 5806 AGGCCT AGG^CCT
Van91I 595 CCATTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1183 TCTAGA T^CTAGA
XhoI 1953 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 2882 GAATTC [GA]^AATT[CT]
3023 AAATTT
ApaI 2649 GGGCCC GGGCC^C
5298 GGGCCC
ApoI 2882 GAATTC [GA]^AATT[CT]
3023 AAATTT
AspI 1768 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2729 GACAGGGTC
AvrII 2715 CCTAGG C^CTAGG
3058 CCTAGG
BamHI 3155 GGATCC G^GATCC
5830 GGATCC
BclI 233 TGATCA T^GATCA
4692 TGATCA
BlnI 2715 CCTAGG C^CTAGG
3058 CCTAGG
BlpI 357 GCTGAGC GC^T[ACGT]AGC
4031 GCTGAGC
Bpu1102I 357 GCTGAGC GC^T[ACGT]AGC
4031 GCTGAGC
BsaWI 79 TCCGGA [AT]^CCGG[AT]
3099 ACCGGT
CelII 357 GCTGAGC GC^T[ACGT]AGC
4031 GCTGAGC
EcoT22I 2756 ATGCAT ATGCA^T
4077 ATGCAT
FbaI 233 TGATCA T^GATCA
4692 TGATCA
NdeI 1436 CATATG CA^TATG
2527 CATATG
NsiI 2756 ATGCAT ATGCA^T
4077 ATGCAT
PflFI 1768 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2729 GACAGGGTC
SacI 4706 GAGCTC GAGCT^C
4915 GAGCTC
SexAI 134 ACCAGGT A^CC[AT]GGT
2580 ACCAGGT
Tth111I 1768 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2729 GACAGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AhdI AluI AlwI AlwNI AocI
Aor51HI Asp700I AspEI AspHI AvaI
AvaII AviII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcgI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI XmnI