################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII AflII AscI AseI AsnI BbeI BfrI BsaBI BsiWI BspDI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRV FseI HindIII HpaI KasI MamI MfeI MluI MunI NarI NheI NotI NruI NspV PacI PmeI PshBI PvuI RsrII SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 5191 GGTACC G^GTACC AccIII 79 TCCGGA T^CCGGA AclI 5974 AACGTT AA^CGTT AcyI 3298 GACGCC G[GA]^CG[CT]C AgeI 3099 ACCGGT A^CCGGT Alw44I 103 GTGCAC G^TGCAC ApaLI 103 GTGCAC G^TGCAC Asp718I 5191 GGTACC G^GTACC BciVI 307 GGATAC GTATCC(6/5) BglI 3459 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 3298 GACGCC G[GA]^CG[CT]C BseAI 79 TCCGGA T^CCGGA BsiEI 4012 CGACCG CG[GA][CT]^CG BsmBI 5866 GAGACG CGTCTC(1/5) BsmI 2229 GAATGC GAATGC(1/-1) Bsp1407I 4113 TGTACA T^GTACA BspEI 79 TCCGGA T^CCGGA BsrGI 4113 TGTACA T^GTACA BssSI 88 CACGAG CACGAG(-5/-1) BstAPI 4283 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EcoRI 2882 GAATTC G^AATTC Hin1I 3298 GACGCC G[GA]^CG[CT]C KpnI 5191 GGTACC GGTAC^C KspI 35 CCGCGG CCGC^GG MroI 79 TCCGGA T^CCGGA NaeI 4047 GCCGGC GCC^GGC NgoMIV 4047 GCCGGC G^CCGGC PaeR7I 1953 CTCGAG C^TCGAG PflMI 595 CCATTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 3099 ACCGGT A^CCGGT PshAI 4511 GACGTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 5974 AACGTT AA^CGTT SacII 35 CCGCGG CCGC^GG SalI 1270 GTCGAC G^TCGAC ScaI 4338 AGTACT AGT^ACT SspBI 4113 TGTACA T^GTACA SspI 6072 AATATT AAT^ATT StuI 5806 AGGCCT AGG^CCT Van91I 595 CCATTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1183 TCTAGA T^CTAGA XhoI 1953 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 2882 GAATTC [GA]^AATT[CT] 3023 AAATTT ApaI 2649 GGGCCC GGGCC^C 5298 GGGCCC ApoI 2882 GAATTC [GA]^AATT[CT] 3023 AAATTT AspI 1768 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2729 GACAGGGTC AvrII 2715 CCTAGG C^CTAGG 3058 CCTAGG BamHI 3155 GGATCC G^GATCC 5830 GGATCC BclI 233 TGATCA T^GATCA 4692 TGATCA BlnI 2715 CCTAGG C^CTAGG 3058 CCTAGG BlpI 357 GCTGAGC GC^T[ACGT]AGC 4031 GCTGAGC Bpu1102I 357 GCTGAGC GC^T[ACGT]AGC 4031 GCTGAGC BsaWI 79 TCCGGA [AT]^CCGG[AT] 3099 ACCGGT CelII 357 GCTGAGC GC^T[ACGT]AGC 4031 GCTGAGC EcoT22I 2756 ATGCAT ATGCA^T 4077 ATGCAT FbaI 233 TGATCA T^GATCA 4692 TGATCA NdeI 1436 CATATG CA^TATG 2527 CATATG NsiI 2756 ATGCAT ATGCA^T 4077 ATGCAT PflFI 1768 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2729 GACAGGGTC SacI 4706 GAGCTC GAGCT^C 4915 GAGCTC SexAI 134 ACCAGGT A^CC[AT]GGT 2580 ACCAGGT Tth111I 1768 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2729 GACAGGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AccI AccII AciI AfaI AflIII AhdI AluI AlwI AlwNI AocI Aor51HI Asp700I AspEI AspHI AvaI AvaII AviII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcgI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI XmnI