##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AclI AflII AhdI Aor51HI ApaI
AscI AspEI AspI AviII BbrPI
BcgI BclI BfrI BglI BsaAI
BsaBI BsiWI BsmBI BssHII Bst1107I
BstZ17I CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
FbaI FseI FspI KspI MamI
MfeI MluI MunI NheI NotI
NruI PacI PflFI PflMI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SbfI ScaI SfiI
SnaBI SphI Sse8387I StuI SwaI
Tth111I Van91I
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1147 GACGTC GACGT^C
Acc65I 1976 GGTACC G^GTACC
AccIII 1440 TCCGGA T^CCGGA
AgeI 70 ACCGGT A^CCGGT
Alw44I 2803 GTGCAC G^TGCAC
ApaLI 2803 GTGCAC G^TGCAC
Asp718I 1976 GGTACC G^GTACC
AvrII 969 CCTAGG C^CTAGG
BalI 3497 TGGCCA TGG^CCA
BamHI 1511 GGATCC G^GATCC
BbeI 2191 GGCGCC GGCGC^C
BbvCI 416 CCTCAGC CCTCAGC(-5/-2)
BciVI 4004 GTATCC GTATCC(6/5)
BlnI 969 CCTAGG C^CTAGG
BsaI 187 GAGACC GGTCTC(1/5)
BseAI 1440 TCCGGA T^CCGGA
BsgI 2390 GTGCAG GTGCAG(16/14)
BsiEI 552 CGGTCG CG[GA][CT]^CG
Bsp1407I 1957 TGTACA T^GTACA
BspEI 1440 TCCGGA T^CCGGA
BsrDI 1869 CATTGC GCAATG(2/0)
BsrGI 1957 TGTACA T^GTACA
BstEII 1650 GGTGACC G^GT[ACGT]ACC
BstPI 1650 GGTGACC G^GT[ACGT]ACC
BstXI 2336 CCACTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 4688 TTTAAA TTT^AAA
DrdI 3320 GACACCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1650 GGTGACC G^GT[ACGT]ACC
EcoRV 3091 GATATC GAT^ATC
EcoT22I 3111 ATGCAT ATGCA^T
KasI 2191 GGCGCC G^GCGCC
KpnI 1976 GGTACC GGTAC^C
MluNI 3497 TGGCCA TGG^CCA
MroI 1440 TCCGGA T^CCGGA
MscI 3497 TGGCCA TGG^CCA
NaeI 2696 GCCGGC GCC^GGC
NarI 2191 GGCGCC GG^CGCC
NcoI 3987 CCATGG C^CATGG
NgoMIV 2696 GCCGGC G^CCGGC
NsiI 3111 ATGCAT ATGCA^T
PaeR7I 2056 CTCGAG C^TCGAG
PinAI 70 ACCGGT A^CCGGT
SapI 1198 GAAGAGC GCTCTTC(1/4)
SexAI 953 ACCAGGT A^CC[AT]GGT
SfoI 2191 GGCGCC GGC^GCC
SgrAI 3840 CACCGGCG C[GA]^CCGG[CT]G
SmaI 2247 CCCGGG CCC^GGG
SspBI 1957 TGTACA T^GTACA
XhoI 2056 CTCGAG C^TCGAG
XmaCI 2247 CCCGGG C^CCGGG
XmaI 2247 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 1147 GACGTC G[GA]^CG[CT]C
2191 GGCGCC
AflIII 116 ACATGT A^C[GA][CT]GT
5025 ACATGT
BlpI 1249 GCTCAGC GC^T[ACGT]AGC
1416 GCTGAGC
Bpu1102I 1249 GCTCAGC GC^T[ACGT]AGC
1416 GCTGAGC
BsaHI 1147 GACGTC G[GA]^CG[CT]C
2191 GGCGCC
BspDI 4072 ATCGAT AT^CGAT
4388 ATCGAT
BspHI 123 TCATGA T^CATGA
3723 TCATGA
BspLU11I 116 ACATGT A^CATGT
5025 ACATGT
BsrBI 1436 CCGCTC CCGCTC(-3/-3)
1785 GAGCGG
BssSI 685 CACGAG CACGAG(-5/-1)
2971 CTCGTG
BstBI 3116 TTCGAA TT^CGAA
4141 TTCGAA
CelII 1249 GCTCAGC GC^T[ACGT]AGC
1416 GCTGAGC
ClaI 4072 ATCGAT AT^CGAT
4388 ATCGAT
HgaI 1334 GACGC GACGC(5/10)
3912 GACGC
Hin1I 1147 GACGTC G[GA]^CG[CT]C
2191 GGCGCC
NdeI 2116 CATATG CA^TATG
2600 CATATG
NspI 116 ACATGT [GA]CATG^[CT]
5025 ACATGT
NspV 3116 TTCGAA TT^CGAA
4141 TTCGAA
PvuII 940 CAGCTG CAG^CTG
1252 CAGCTG
RcaI 123 TCATGA T^CATGA
3723 TCATGA
SacI 52 GAGCTC GAGCT^C
1419 GAGCTC
SalI 2063 GTCGAC G^TCGAC
3066 GTCGAC
SfuI 3116 TTCGAA TT^CGAA
4141 TTCGAA
XbaI 1296 TCTAGA T^CTAGA
2184 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI