################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AclI AflII AhdI Aor51HI ApaI AscI AspEI AspI AviII BbrPI BcgI BclI BfrI BglI BsaAI BsaBI BsiWI BsmBI BssHII Bst1107I BstZ17I CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI FbaI FseI FspI KspI MamI MfeI MluI MunI NheI NotI NruI PacI PflFI PflMI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SnaBI SphI Sse8387I StuI SwaI Tth111I Van91I ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 1147 GACGTC GACGT^C Acc65I 1976 GGTACC G^GTACC AccIII 1440 TCCGGA T^CCGGA AgeI 70 ACCGGT A^CCGGT Alw44I 2803 GTGCAC G^TGCAC ApaLI 2803 GTGCAC G^TGCAC Asp718I 1976 GGTACC G^GTACC AvrII 969 CCTAGG C^CTAGG BalI 3497 TGGCCA TGG^CCA BamHI 1511 GGATCC G^GATCC BbeI 2191 GGCGCC GGCGC^C BbvCI 416 CCTCAGC CCTCAGC(-5/-2) BciVI 4004 GTATCC GTATCC(6/5) BlnI 969 CCTAGG C^CTAGG BsaI 187 GAGACC GGTCTC(1/5) BseAI 1440 TCCGGA T^CCGGA BsgI 2390 GTGCAG GTGCAG(16/14) BsiEI 552 CGGTCG CG[GA][CT]^CG Bsp1407I 1957 TGTACA T^GTACA BspEI 1440 TCCGGA T^CCGGA BsrDI 1869 CATTGC GCAATG(2/0) BsrGI 1957 TGTACA T^GTACA BstEII 1650 GGTGACC G^GT[ACGT]ACC BstPI 1650 GGTGACC G^GT[ACGT]ACC BstXI 2336 CCACTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 4688 TTTAAA TTT^AAA DrdI 3320 GACACCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1650 GGTGACC G^GT[ACGT]ACC EcoRV 3091 GATATC GAT^ATC EcoT22I 3111 ATGCAT ATGCA^T KasI 2191 GGCGCC G^GCGCC KpnI 1976 GGTACC GGTAC^C MluNI 3497 TGGCCA TGG^CCA MroI 1440 TCCGGA T^CCGGA MscI 3497 TGGCCA TGG^CCA NaeI 2696 GCCGGC GCC^GGC NarI 2191 GGCGCC GG^CGCC NcoI 3987 CCATGG C^CATGG NgoMIV 2696 GCCGGC G^CCGGC NsiI 3111 ATGCAT ATGCA^T PaeR7I 2056 CTCGAG C^TCGAG PinAI 70 ACCGGT A^CCGGT SapI 1198 GAAGAGC GCTCTTC(1/4) SexAI 953 ACCAGGT A^CC[AT]GGT SfoI 2191 GGCGCC GGC^GCC SgrAI 3840 CACCGGCG C[GA]^CCGG[CT]G SmaI 2247 CCCGGG CCC^GGG SspBI 1957 TGTACA T^GTACA XhoI 2056 CTCGAG C^TCGAG XmaCI 2247 CCCGGG C^CCGGG XmaI 2247 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 1147 GACGTC G[GA]^CG[CT]C 2191 GGCGCC AflIII 116 ACATGT A^C[GA][CT]GT 5025 ACATGT BlpI 1249 GCTCAGC GC^T[ACGT]AGC 1416 GCTGAGC Bpu1102I 1249 GCTCAGC GC^T[ACGT]AGC 1416 GCTGAGC BsaHI 1147 GACGTC G[GA]^CG[CT]C 2191 GGCGCC BspDI 4072 ATCGAT AT^CGAT 4388 ATCGAT BspHI 123 TCATGA T^CATGA 3723 TCATGA BspLU11I 116 ACATGT A^CATGT 5025 ACATGT BsrBI 1436 CCGCTC CCGCTC(-3/-3) 1785 GAGCGG BssSI 685 CACGAG CACGAG(-5/-1) 2971 CTCGTG BstBI 3116 TTCGAA TT^CGAA 4141 TTCGAA CelII 1249 GCTCAGC GC^T[ACGT]AGC 1416 GCTGAGC ClaI 4072 ATCGAT AT^CGAT 4388 ATCGAT HgaI 1334 GACGC GACGC(5/10) 3912 GACGC Hin1I 1147 GACGTC G[GA]^CG[CT]C 2191 GGCGCC NdeI 2116 CATATG CA^TATG 2600 CATATG NspI 116 ACATGT [GA]CATG^[CT] 5025 ACATGT NspV 3116 TTCGAA TT^CGAA 4141 TTCGAA PvuII 940 CAGCTG CAG^CTG 1252 CAGCTG RcaI 123 TCATGA T^CATGA 3723 TCATGA SacI 52 GAGCTC GAGCT^C 1419 GAGCTC SalI 2063 GTCGAC G^TCGAC 3066 GTCGAC SfuI 3116 TTCGAA TT^CGAA 4141 TTCGAA XbaI 1296 TCTAGA T^CTAGA 2184 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI