##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccII AflII AgeI AhdI AscI
AseI AsnI AspEI BbeI BfrI
BsiWI BspDI BspHI BssHII BstAPI
BstBI BstUI ClaI CpoI DrdI
Eam1105I EcoT22I FseI HindIII HpaI
KasI KspI MluI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
RcaI RsrII SacII SalI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 1299 GGTACC G^GTACC
AccIII 1698 TCCGGA T^CCGGA
AclI 5598 AACGTT AA^CGTT
Aor51HI 140 AGCGCT AGC^GCT
Asp718I 1299 GGTACC G^GTACC
AvrII 6443 CCTAGG C^CTAGG
BamHI 2857 GGATCC G^GATCC
BbvCI 5524 GCTGAGG CCTCAGC(-5/-2)
BciVI 548 GGATAC GTATCC(6/5)
BglII 432 AGATCT A^GATCT
BlnI 6443 CCTAGG C^CTAGG
BsaBI 2969 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1698 TCCGGA T^CCGGA
BsmI 970 GAATGC GAATGC(1/-1)
BspEI 1698 TCCGGA T^CCGGA
BspLU11I 398 ACATGT A^CATGT
DraIII 4745 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 5685 CGGCCG C^GGCCG
EclXI 5685 CGGCCG C^GGCCG
Eco47III 140 AGCGCT AGC^GCT
Eco52I 5685 CGGCCG C^GGCCG
EcoRI 410 GAATTC G^AATTC
EcoRV 1266 GATATC GAT^ATC
HincII 3995 GTTGAC GT[CT]^[GA]AC
HindII 3995 GTTGAC GT[CT]^[GA]AC
KpnI 1299 GGTACC GGTAC^C
MamI 2969 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6291 CAATTG C^AATTG
MroI 1698 TCCGGA T^CCGGA
MunI 6291 CAATTG C^AATTG
PaeR7I 3419 CTCGAG C^TCGAG
PshAI 2455 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5598 AACGTT AA^CGTT
PvuI 4037 CGATCG CGAT^CG
SbfI 2702 CCTGCAGG CCTGCA^GG
SfiI 3243 GGCCGGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2608 CCCGGG CCC^GGG
Sse8387I 2702 CCTGCAGG CCTGCA^GG
StuI 2619 AGGCCT AGG^CCT
SwaI 6211 ATTTAAAT ATTT^AAAT
XhoI 3419 CTCGAG C^TCGAG
XmaCI 2608 CCCGGG C^CCGGG
XmaI 2608 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 2135 GACGTC GACGT^C
2351 GACGTC
Alw44I 453 GTGCAC G^TGCAC
1206 GTGCAC
AocI 988 CCTCAGG CC^T[ACGT]AGG
3225 CCTGAGG
ApaI 2726 GGGCCC GGGCC^C
5929 GGGCCC
ApaLI 453 GTGCAC G^TGCAC
1206 GTGCAC
Asp700I 1050 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5971 GAAATGATTC
AviII 1855 TGCGCA TGC^GCA
3590 TGCGCA
BcgI 865 GCATCTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3562 GCAGCCGGCTCG
BclI 124 TGATCA T^GATCA
2381 TGATCA
BsiEI 4037 CGATCG CG[GA][CT]^CG
5685 CGGCCG
BsmBI 2353 CGTCTC CGTCTC(1/5)
4915 CGTCTC
Bsp1407I 829 TGTACA T^GTACA
3073 TGTACA
BspMI 246 ACCTGC ACCTGC(4/8)
738 ACCTGC
BsrBI 680 CCGCTC CCGCTC(-3/-3)
87 GAGCGG
BsrGI 829 TGTACA T^GTACA
3073 TGTACA
Bst1107I 2299 GTATAC GTA^TAC
3665 GTATAC
BstEII 3602 GGTCACC G^GT[ACGT]ACC
5324 GGTAACC
BstPI 3602 GGTCACC G^GT[ACGT]ACC
5324 GGTAACC
BstXI 538 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2920 CCACCAACATGG
BstZ17I 2299 GTATAC GTA^TAC
3665 GTATAC
Bsu36I 988 CCTCAGG CC^T[ACGT]AGG
3225 CCTGAGG
Eco81I 988 CCTCAGG CC^T[ACGT]AGG
3225 CCTGAGG
EcoNI 686 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3143 CCTTCAGGAGG
EcoO65I 3602 GGTCACC G^GT[ACGT]ACC
5324 GGTAACC
FbaI 124 TGATCA T^GATCA
2381 TGATCA
FspI 1855 TGCGCA TGC^GCA
3590 TGCGCA
SacI 1079 GAGCTC GAGCT^C
5101 GAGCTC
SapI 1164 GAAGAGC GCTCTTC(1/4)
3491 GAAGAGC
SspBI 829 TGTACA T^GTACA
3073 TGTACA
XcmI 1667 CCAGGGCCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5135 CCACCCAATGGGTGG
XmnI 1050 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5971 GAAATGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII