##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AclI AhdI Aor51HI AscI AspEI
BalI BbrPI BclI BsaAI BsiEI
BsiWI BspDI BspHI BspMI BsrBI
BssHII BssSI Bst1107I BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FbaI
FseI HincII HindII HpaI KspI
MfeI MluI MluNI MscI MunI
NaeI NdeI NgoMIV NotI NruI
PacI PaeR7I PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 2759 GACGTC GACGT^C
Acc65I 2054 GGTACC G^GTACC
AccI 69 GTAGAC GT^[AC][GT]AC
AflII 2462 CTTAAG C^TTAAG
AgeI 2686 ACCGGT A^CCGGT
ApaI 3796 GGGCCC GGGCC^C
Asp718I 2054 GGTACC G^GTACC
AspI 3866 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 7 TGCGCA TGC^GCA
AvrII 1964 CCTAGG C^CTAGG
BbeI 516 GGCGCC GGCGC^C
BbvCI 2079 CCTCAGC CCTCAGC(-5/-2)
BcgI 1060 CGACCCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4332 GGATAC GTATCC(6/5)
BcnI 3013 CCCGG CC^[GC]GG
BfrI 2462 CTTAAG C^TTAAG
BglII 1289 AGATCT A^GATCT
BlnI 1964 CCTAGG C^CTAGG
BsaI 3165 GAGACC GGTCTC(1/5)
BspLU11I 4561 ACATGT A^CATGT
BsrDI 300 GCAATG GCAATG(2/0)
BstAPI 1308 GCACAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3139 GGTCACC G^GT[ACGT]ACC
BstPI 3139 GGTCACC G^GT[ACGT]ACC
BstXI 3535 CCACATCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2818 TTTAAA TTT^AAA
DrdI 2229 GACGGCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 190 TGGCCG [CT]^GGCC[GA]
EcoO65I 3139 GGTCACC G^GT[ACGT]ACC
EcoT22I 1456 ATGCAT ATGCA^T
FspI 7 TGCGCA TGC^GCA
HgaI 1679 GCGTC GACGC(5/10)
KasI 516 GGCGCC G^GCGCC
KpnI 2054 GGTACC GGTAC^C
NarI 516 GGCGCC GG^CGCC
NciI 3013 CCCGG CC^[GC]GG
NheI 47 GCTAGC G^CTAGC
NsiI 1456 ATGCAT ATGCA^T
PflFI 3866 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2686 ACCGGT A^CCGGT
PvuII 2133 CAGCTG CAG^CTG
SacI 3608 GAGCTC GAGCT^C
SfoI 516 GGCGCC GGC^GCC
SphI 2772 GCATGC GCATG^C
SspI 4571 AATATT AAT^ATT
StuI 4617 AGGCCT AGG^CCT
Tth111I 3866 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 35 CGCG CG^CG
52 CGCG
AccIII 139 TCCGGA T^CCGGA
1346 TCCGGA
AcyI 516 GGCGCC G[GA]^CG[CT]C
2759 GACGTC
AflIII 65 ACGTGT A^C[GA][CT]GT
4561 ACATGT
Alw44I 1664 GTGCAC G^TGCAC
3193 GTGCAC
ApaLI 1664 GTGCAC G^TGCAC
3193 GTGCAC
AseI 3443 ATTAAT AT^TAAT
4535 ATTAAT
AsnI 3443 ATTAAT AT^TAAT
4535 ATTAAT
AvaI 1173 CCCGAG C^[CT]CG[GA]G
3331 CCCGAG
BamHI 2467 GGATCC G^GATCC
4131 GGATCC
BglI 2105 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3206 GCCTCCATGGC
BlpI 3308 GCTAAGC GC^T[ACGT]AGC
4447 GCTGAGC
Bpu1102I 3308 GCTAAGC GC^T[ACGT]AGC
4447 GCTGAGC
BsaBI 1648 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3071 GATAGCCATC
BsaHI 516 GGCGCC G[GA]^CG[CT]C
2759 GACGTC
BseAI 139 TCCGGA T^CCGGA
1346 TCCGGA
BsmBI 3090 CGTCTC CGTCTC(1/5)
62 GAGACG
BsmI 30 GCATTC GAATGC(1/-1)
1518 GCATTC
BsoBI 1173 CCCGAG C^[CT]CG[GA]G
3331 CCCGAG
Bsp1407I 2834 TGTACA T^GTACA
4770 TGTACA
BspEI 139 TCCGGA T^CCGGA
1346 TCCGGA
BsrFI 538 GCCGGT [GA]^CCGG[CT]
2686 ACCGGT
BsrGI 2834 TGTACA T^GTACA
4770 TGTACA
BstUI 35 CGCG CG^CG
52 CGCG
CelII 3308 GCTAAGC GC^T[ACGT]AGC
4447 GCTGAGC
Cfr10I 538 GCCGGT [GA]^CCGG[CT]
2686 ACCGGT
DraIII 3750 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4394 CACAAGGTG
Hin1I 516 GGCGCC G[GA]^CG[CT]C
2759 GACGTC
MamI 1648 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3071 GATAGCCATC
MroI 139 TCCGGA T^CCGGA
1346 TCCGGA
MspA1I 1396 CAGCGG C[AC]G^C[GT]G
2133 CAGCTG
MvnI 35 CGCG CG^CG
52 CGCG
PflMI 3812 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3939 CCAGTGAGTGG
PshAI 939 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1674 GACTAGCGTC
PshBI 3443 ATTAAT AT^TAAT
4535 ATTAAT
PstI 3282 CTGCAG CTGCA^G
4752 CTGCAG
SapI 2737 GAAGAGC GCTCTTC(1/4)
2485 GCTCTTC
SspBI 2834 TGTACA T^GTACA
4770 TGTACA
Van91I 3812 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3939 CCAGTGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstBI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI NspV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI