################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AclI AhdI Aor51HI AscI AspEI BalI BbrPI BclI BsaAI BsiEI BsiWI BspDI BspHI BspMI BsrBI BssHII BssSI Bst1107I BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FbaI FseI HincII HindII HpaI KspI MfeI MluI MluNI MscI MunI NaeI NdeI NgoMIV NotI NruI PacI PaeR7I PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 2759 GACGTC GACGT^C Acc65I 2054 GGTACC G^GTACC AccI 69 GTAGAC GT^[AC][GT]AC AflII 2462 CTTAAG C^TTAAG AgeI 2686 ACCGGT A^CCGGT ApaI 3796 GGGCCC GGGCC^C Asp718I 2054 GGTACC G^GTACC AspI 3866 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 7 TGCGCA TGC^GCA AvrII 1964 CCTAGG C^CTAGG BbeI 516 GGCGCC GGCGC^C BbvCI 2079 CCTCAGC CCTCAGC(-5/-2) BcgI 1060 CGACCCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4332 GGATAC GTATCC(6/5) BcnI 3013 CCCGG CC^[GC]GG BfrI 2462 CTTAAG C^TTAAG BglII 1289 AGATCT A^GATCT BlnI 1964 CCTAGG C^CTAGG BsaI 3165 GAGACC GGTCTC(1/5) BspLU11I 4561 ACATGT A^CATGT BsrDI 300 GCAATG GCAATG(2/0) BstAPI 1308 GCACAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3139 GGTCACC G^GT[ACGT]ACC BstPI 3139 GGTCACC G^GT[ACGT]ACC BstXI 3535 CCACATCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 2818 TTTAAA TTT^AAA DrdI 2229 GACGGCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 190 TGGCCG [CT]^GGCC[GA] EcoO65I 3139 GGTCACC G^GT[ACGT]ACC EcoT22I 1456 ATGCAT ATGCA^T FspI 7 TGCGCA TGC^GCA HgaI 1679 GCGTC GACGC(5/10) KasI 516 GGCGCC G^GCGCC KpnI 2054 GGTACC GGTAC^C NarI 516 GGCGCC GG^CGCC NciI 3013 CCCGG CC^[GC]GG NheI 47 GCTAGC G^CTAGC NsiI 1456 ATGCAT ATGCA^T PflFI 3866 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2686 ACCGGT A^CCGGT PvuII 2133 CAGCTG CAG^CTG SacI 3608 GAGCTC GAGCT^C SfoI 516 GGCGCC GGC^GCC SphI 2772 GCATGC GCATG^C SspI 4571 AATATT AAT^ATT StuI 4617 AGGCCT AGG^CCT Tth111I 3866 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccII 35 CGCG CG^CG 52 CGCG AccIII 139 TCCGGA T^CCGGA 1346 TCCGGA AcyI 516 GGCGCC G[GA]^CG[CT]C 2759 GACGTC AflIII 65 ACGTGT A^C[GA][CT]GT 4561 ACATGT Alw44I 1664 GTGCAC G^TGCAC 3193 GTGCAC ApaLI 1664 GTGCAC G^TGCAC 3193 GTGCAC AseI 3443 ATTAAT AT^TAAT 4535 ATTAAT AsnI 3443 ATTAAT AT^TAAT 4535 ATTAAT AvaI 1173 CCCGAG C^[CT]CG[GA]G 3331 CCCGAG BamHI 2467 GGATCC G^GATCC 4131 GGATCC BglI 2105 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3206 GCCTCCATGGC BlpI 3308 GCTAAGC GC^T[ACGT]AGC 4447 GCTGAGC Bpu1102I 3308 GCTAAGC GC^T[ACGT]AGC 4447 GCTGAGC BsaBI 1648 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3071 GATAGCCATC BsaHI 516 GGCGCC G[GA]^CG[CT]C 2759 GACGTC BseAI 139 TCCGGA T^CCGGA 1346 TCCGGA BsmBI 3090 CGTCTC CGTCTC(1/5) 62 GAGACG BsmI 30 GCATTC GAATGC(1/-1) 1518 GCATTC BsoBI 1173 CCCGAG C^[CT]CG[GA]G 3331 CCCGAG Bsp1407I 2834 TGTACA T^GTACA 4770 TGTACA BspEI 139 TCCGGA T^CCGGA 1346 TCCGGA BsrFI 538 GCCGGT [GA]^CCGG[CT] 2686 ACCGGT BsrGI 2834 TGTACA T^GTACA 4770 TGTACA BstUI 35 CGCG CG^CG 52 CGCG CelII 3308 GCTAAGC GC^T[ACGT]AGC 4447 GCTGAGC Cfr10I 538 GCCGGT [GA]^CCGG[CT] 2686 ACCGGT DraIII 3750 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 4394 CACAAGGTG Hin1I 516 GGCGCC G[GA]^CG[CT]C 2759 GACGTC MamI 1648 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3071 GATAGCCATC MroI 139 TCCGGA T^CCGGA 1346 TCCGGA MspA1I 1396 CAGCGG C[AC]G^C[GT]G 2133 CAGCTG MvnI 35 CGCG CG^CG 52 CGCG PflMI 3812 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3939 CCAGTGAGTGG PshAI 939 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1674 GACTAGCGTC PshBI 3443 ATTAAT AT^TAAT 4535 ATTAAT PstI 3282 CTGCAG CTGCA^G 4752 CTGCAG SapI 2737 GAAGAGC GCTCTTC(1/4) 2485 GCTCTTC SspBI 2834 TGTACA T^GTACA 4770 TGTACA Van91I 3812 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3939 CCAGTGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstBI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI NspV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI