##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AcsI AflII AflIII
AgeI AhdI ApoI AscI AseI
AsnI Asp718I AspEI AviII BbeI
BbrPI BclI BfrI BsiWI BsmI
Bsp1407I BspLU11I BsrGI BssHII Bst1107I
BstZ17I CpoI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MluI NarI NdeI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
RsrII SacII SalI SfoI SgrAI
SmaI SnaBI SpeI SspBI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 693 GTCTAC GT^[AC][GT]AC
AclI 6399 AACGTT AA^CGTT
AcyI 5483 GACGCC G[GA]^CG[CT]C
Alw44I 228 GTGCAC G^TGCAC
ApaLI 228 GTGCAC G^TGCAC
Asp700I 4458 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 6142 GGATCC G^GATCC
BcgI 6631 CGACACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 315 CACGTA [CT]AC^GT[GA]
BsaBI 5545 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5483 GACGCC G[GA]^CG[CT]C
BsiEI 6619 CGATCG CG[GA][CT]^CG
BspDI 6617 ATCGAT AT^CGAT
BssSI 4347 CACGAG CACGAG(-5/-1)
BstBI 4455 TTCGAA TT^CGAA
BstEII 6098 GGTAACC G^GT[ACGT]ACC
BstPI 6098 GGTAACC G^GT[ACGT]ACC
ClaI 6617 ATCGAT AT^CGAT
DraI 2156 TTTAAA TTT^AAA
EcoO65I 6098 GGTAACC G^GT[ACGT]ACC
Hin1I 5483 GACGCC G[GA]^CG[CT]C
HincII 3815 GTCAAC GT[CT]^[GA]AC
HindII 3815 GTCAAC GT[CT]^[GA]AC
MamI 5545 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1079 CAATTG C^AATTG
MunI 1079 CAATTG C^AATTG
NspV 4455 TTCGAA TT^CGAA
PpuMI 975 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 6399 AACGTT AA^CGTT
PvuI 6619 CGATCG CGAT^CG
SbfI 2015 CCTGCAGG CCTGCA^GG
ScaI 3918 AGTACT AGT^ACT
SexAI 5739 ACCAGGT A^CC[AT]GGT
SfiI 4958 GGCCTCATAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4455 TTCGAA TT^CGAA
Sse8387I 2015 CCTGCAGG CCTGCA^GG
SspI 5999 AATATT AAT^ATT
XmnI 4458 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 4398 CGCG CG^CG
6593 CGCG
AccIII 1187 TCCGGA T^CCGGA
2731 TCCGGA
ApaI 350 GGGCCC GGGCC^C
4536 GGGCCC
BciVI 1339 GGATAC GTATCC(6/5)
1503 GGATAC
BglII 1128 AGATCT A^GATCT
3992 AGATCT
BsaWI 1187 TCCGGA [AT]^CCGG[AT]
2731 TCCGGA
BseAI 1187 TCCGGA T^CCGGA
2731 TCCGGA
BspEI 1187 TCCGGA T^CCGGA
2731 TCCGGA
BsrDI 5013 GCAATG GCAATG(2/0)
5910 GCAATG
BstUI 4398 CGCG CG^CG
6593 CGCG
DrdI 23 GACAGCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4712 GACTGTTCTGTC
HindIII 1207 AAGCTT A^AGCTT
2952 AAGCTT
MroI 1187 TCCGGA T^CCGGA
2731 TCCGGA
MvnI 4398 CGCG CG^CG
6593 CGCG
NcoI 2795 CCATGG C^CATGG
5799 CCATGG
SacI 3252 GAGCTC GAGCT^C
5900 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI Aor51HI AspHI AspI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NheI NlaIII NlaIV
NspI PflFI PflMI PleI PstI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoII