##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AvaI AviII
BbeI BbrPI BbvCI BcgI BciVI
BclI BglI BlpI Bpu1102I BsaHI
BsaWI BseAI BsiWI BsoBI BspDI
BspEI BspHI BsrBI BssHII BssSI
CelII ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI Psp1406I RcaI RsrII SacII
SalI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI StuI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 2616 CGCG CG^CG
AflII 4478 CTTAAG C^TTAAG
AocI 1254 CCTCAGG CC^T[ACGT]AGG
ApaI 634 GGGCCC GGGCC^C
AspI 3796 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3854 CCTAGG C^CTAGG
BanI 3538 GGTGCC G^G[CT][GA]CC
BcnI 4541 CCCGG CC^[GC]GG
BfrI 4478 CTTAAG C^TTAAG
BlnI 3854 CCTAGG C^CTAGG
BpmI 2584 CTCCAG CTGGAG(16/14)
BsaAI 260 CACGTA [CT]AC^GT[GA]
BsaBI 2679 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2526 GGTCTC GGTCTC(1/5)
BseRI 756 GAGGAG GAGGAG(10/8)
BsiEI 4575 CGATCG CG[GA][CT]^CG
BsmI 4624 GCATTC GAATGC(1/-1)
Bsp1407I 4842 TGTACA T^GTACA
BsrFI 4152 GCCGGT [GA]^CCGG[CT]
BsrGI 4842 TGTACA T^GTACA
BstAPI 4108 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1327 TTCGAA TT^CGAA
BstEII 2758 GGTCACC G^GT[ACGT]ACC
BstPI 2758 GGTCACC G^GT[ACGT]ACC
BstUI 2616 CGCG CG^CG
Bsu36I 1254 CCTCAGG CC^T[ACGT]AGG
CfoI 2615 GCGC GCG^C
Cfr10I 4152 GCCGGT [GA]^CCGG[CT]
DraIII 2348 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1254 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2758 GGTCACC G^GT[ACGT]ACC
FauI 1352 GCGGG CCCGC(4/6)
HhaI 2615 GCGC GCG^C
HinP1I 2615 GCGC G^CGC
HincII 4702 GTTAAC GT[CT]^[GA]AC
HindII 4702 GTTAAC GT[CT]^[GA]AC
HpaI 4702 GTTAAC GTT^AAC
MamI 2679 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2616 CGCG CG^CG
NciI 4541 CCCGG CC^[GC]GG
NdeI 2889 CATATG CA^TATG
NspV 1327 TTCGAA TT^CGAA
PflFI 3796 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2347 CCACTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2498 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4575 CGATCG CGAT^CG
SacI 2786 GAGCTC GAGCT^C
SbfI 1203 CCTGCAGG CCTGCA^GG
SfuI 1327 TTCGAA TT^CGAA
SphI 1240 GCATGC GCATG^C
Sse8387I 1203 CCTGCAGG CCTGCA^GG
SspBI 4842 TGTACA T^GTACA
SwaI 2158 ATTTAAAT ATTT^AAAT
Tth111I 3796 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2347 CCACTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1312 ACATGT A^C[GA][CT]GT
4317 ACATGT
AspHI 2786 GAGCTC G[AT]GC[AT]^C
3830 GTGCTC
AvaII 900 GGTCC G^G[AT]CC
1292 GGACC
BamHI 620 GGATCC G^GATCC
3387 GGATCC
BbsI 1184 GAAGAC GAAGAC(2/6)
3887 GTCTTC
BpuAI 1184 GAAGAC GAAGAC(2/6)
3887 GTCTTC
BsgI 3875 CTGCAC GTGCAG(16/14)
2537 GTGCAG
BsiHKAI 2786 GAGCTC G[AT]GC[AT]^C
3830 GTGCTC
BsmBI 4565 CGTCTC CGTCTC(1/5)
2996 GAGACG
BspLU11I 1312 ACATGT A^CATGT
4317 ACATGT
BspMI 547 ACCTGC ACCTGC(4/8)
1202 ACCTGC
Bst1107I 556 GTATAC GTA^TAC
2900 GTATAC
BstXI 1282 CCACAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1996 CCATGGTTATGG
BstZ17I 556 GTATAC GTA^TAC
2900 GTATAC
DraII 633 AGGGCCC [GA]G^G[ACGT]CC[CT]
634 GGGCCCT
DsaI 22 CCATGG C^C[GA][CT]GG
1996 CCATGG
EcoO109I 633 AGGGCCC [GA]G^G[ACGT]CC[CT]
634 GGGCCCT
HapII 4153 CCGG C^CGG
4542 CCGG
HpaII 4153 CCGG C^CGG
4542 CCGG
MspA1I 1766 CAGCTG C[AC]G^C[GT]G
2546 CAGCTG
MspI 4153 CCGG C^CGG
4542 CCGG
NcoI 22 CCATGG C^CATGG
1996 CCATGG
PvuII 1766 CAGCTG CAG^CTG
2546 CAGCTG
SapI 3552 GAAGAGC GCTCTTC(1/4)
4204 GCTCTTC
SpeI 3141 ACTAGT A^CTAGT
4472 ACTAGT
XcmI 87 CCATCAGCCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1018 CCATTAAGTGTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I BalI BanII BbvI BfaI
BglII BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DrdI
EaeI EarI EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PacI PleI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI