##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflIII AgeI Alw44I ApaLI
AscI Asp700I AvrII BamHI BbeI
BbrPI BclI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BspEI BspLU11I BsrBI BssHII
Bst1107I BstBI BstUI BstZ17I CelII
CpoI DrdI EagI EclXI Eco52I
EcoNI EcoT22I FbaI FseI Hin1I
HpaI KasI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I StuI SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 781 GGTACC G^GTACC
AflII 3512 CTTAAG C^TTAAG
AhdI 2619 GACTAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 606 AGCGCT AGC^GCT
ApaI 3583 GGGCCC GGGCC^C
AseI 3540 ATTAAT AT^TAAT
AsnI 3540 ATTAAT AT^TAAT
Asp718I 781 GGTACC G^GTACC
AspEI 2619 GACTAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 672 TGCGCA TGC^GCA
BalI 3273 TGGCCA TGG^CCA
BbvCI 571 CCTCAGC CCTCAGC(-5/-2)
BcgI 32 GCAGACATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3512 CTTAAG C^TTAAG
BglII 546 AGATCT A^GATCT
BsmBI 290 CGTCTC CGTCTC(1/5)
BspDI 970 ATCGAT AT^CGAT
BsrFI 422 ACCGGC [GA]^CCGG[CT]
BstAPI 2158 GCAGTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 418 GGTGACC G^GT[ACGT]ACC
BstPI 418 GGTGACC G^GT[ACGT]ACC
Cfr10I 422 ACCGGC [GA]^CCGG[CT]
ClaI 970 ATCGAT AT^CGAT
Eam1105I 2619 GACTAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 606 AGCGCT AGC^GCT
EcoO65I 418 GGTGACC G^GT[ACGT]ACC
FspI 672 TGCGCA TGC^GCA
HaeII 606 AGCGCT [GA]GCGC^[CT]
HgaI 588 GCGTC GACGC(5/10)
HindIII 4392 AAGCTT A^AGCTT
KpnI 781 GGTACC GGTAC^C
MluNI 3273 TGGCCA TGG^CCA
MscI 3273 TGGCCA TGG^CCA
NcoI 344 CCATGG C^CATGG
PshAI 3326 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3540 ATTAAT AT^TAAT
SacI 2298 GAGCTC GAGCT^C
SmaI 2667 CCCGGG CCC^GGG
SspI 4512 AATATT AAT^ATT
XbaI 2842 TCTAGA T^CTAGA
XmaCI 2667 CCCGGG C^CCGGG
XmaI 2667 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspI 1879 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4021 GACCTTGTC
AvaI 1008 CTCGGG C^[CT]CG[GA]G
2667 CCCGGG
BciVI 1019 GGATAC GTATCC(6/5)
1299 GTATCC
BglI 1793 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1976 GCCCATTTGGC
BsaWI 241 TCCGGT [AT]^CCGG[AT]
807 ACCGGA
BsgI 197 CTGCAC GTGCAG(16/14)
1270 GTGCAG
BsmI 2017 GAATGC GAATGC(1/-1)
4480 GCATTC
BsoBI 1008 CTCGGG C^[CT]CG[GA]G
2667 CCCGGG
Bsp1407I 995 TGTACA T^GTACA
3610 TGTACA
BsrDI 516 GCAATG GCAATG(2/0)
4044 GCAATG
BsrGI 995 TGTACA T^GTACA
3610 TGTACA
EaeI 936 CGGCCA [CT]^GGCC[GA]
3273 TGGCCA
EarI 412 GAAGAG CTCTTC(1/4)
1699 GAAGAG
EcoRV 871 GATATC GAT^ATC
4159 GATATC
Ksp632I 412 GAAGAG CTCTTC(1/4)
1699 GAAGAG
MaeII 117 ACGT A^CGT
441 ACGT
NheI 1013 GCTAGC G^CTAGC
3360 GCTAGC
PflFI 1879 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4021 GACCTTGTC
SmlI 982 CTCAAG C^T[CT][GA]AG
3512 CTTAAG
SphI 1307 GCATGC GCATG^C
1346 GCATGC
SspBI 995 TGTACA T^GTACA
3610 TGTACA
Tth111I 1879 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4021 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BssSI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII