################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflIII AgeI Alw44I ApaLI AscI Asp700I AvrII BamHI BbeI BbrPI BclI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BspEI BspLU11I BsrBI BssHII Bst1107I BstBI BstUI BstZ17I CelII CpoI DrdI EagI EclXI Eco52I EcoNI EcoT22I FbaI FseI Hin1I HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I StuI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 781 GGTACC G^GTACC AflII 3512 CTTAAG C^TTAAG AhdI 2619 GACTAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 606 AGCGCT AGC^GCT ApaI 3583 GGGCCC GGGCC^C AseI 3540 ATTAAT AT^TAAT AsnI 3540 ATTAAT AT^TAAT Asp718I 781 GGTACC G^GTACC AspEI 2619 GACTAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 672 TGCGCA TGC^GCA BalI 3273 TGGCCA TGG^CCA BbvCI 571 CCTCAGC CCTCAGC(-5/-2) BcgI 32 GCAGACATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 3512 CTTAAG C^TTAAG BglII 546 AGATCT A^GATCT BsmBI 290 CGTCTC CGTCTC(1/5) BspDI 970 ATCGAT AT^CGAT BsrFI 422 ACCGGC [GA]^CCGG[CT] BstAPI 2158 GCAGTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 418 GGTGACC G^GT[ACGT]ACC BstPI 418 GGTGACC G^GT[ACGT]ACC Cfr10I 422 ACCGGC [GA]^CCGG[CT] ClaI 970 ATCGAT AT^CGAT Eam1105I 2619 GACTAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 606 AGCGCT AGC^GCT EcoO65I 418 GGTGACC G^GT[ACGT]ACC FspI 672 TGCGCA TGC^GCA HaeII 606 AGCGCT [GA]GCGC^[CT] HgaI 588 GCGTC GACGC(5/10) HindIII 4392 AAGCTT A^AGCTT KpnI 781 GGTACC GGTAC^C MluNI 3273 TGGCCA TGG^CCA MscI 3273 TGGCCA TGG^CCA NcoI 344 CCATGG C^CATGG PshAI 3326 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3540 ATTAAT AT^TAAT SacI 2298 GAGCTC GAGCT^C SmaI 2667 CCCGGG CCC^GGG SspI 4512 AATATT AAT^ATT XbaI 2842 TCTAGA T^CTAGA XmaCI 2667 CCCGGG C^CCGGG XmaI 2667 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AspI 1879 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4021 GACCTTGTC AvaI 1008 CTCGGG C^[CT]CG[GA]G 2667 CCCGGG BciVI 1019 GGATAC GTATCC(6/5) 1299 GTATCC BglI 1793 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1976 GCCCATTTGGC BsaWI 241 TCCGGT [AT]^CCGG[AT] 807 ACCGGA BsgI 197 CTGCAC GTGCAG(16/14) 1270 GTGCAG BsmI 2017 GAATGC GAATGC(1/-1) 4480 GCATTC BsoBI 1008 CTCGGG C^[CT]CG[GA]G 2667 CCCGGG Bsp1407I 995 TGTACA T^GTACA 3610 TGTACA BsrDI 516 GCAATG GCAATG(2/0) 4044 GCAATG BsrGI 995 TGTACA T^GTACA 3610 TGTACA EaeI 936 CGGCCA [CT]^GGCC[GA] 3273 TGGCCA EarI 412 GAAGAG CTCTTC(1/4) 1699 GAAGAG EcoRV 871 GATATC GAT^ATC 4159 GATATC Ksp632I 412 GAAGAG CTCTTC(1/4) 1699 GAAGAG MaeII 117 ACGT A^CGT 441 ACGT NheI 1013 GCTAGC G^CTAGC 3360 GCTAGC PflFI 1879 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4021 GACCTTGTC SmlI 982 CTCAAG C^T[CT][GA]AG 3512 CTTAAG SphI 1307 GCATGC GCATG^C 1346 GCATGC SspBI 995 TGTACA T^GTACA 3610 TGTACA Tth111I 1879 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4021 GACCTTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII