##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
Alw44I ApaLI AscI AvrII BbeI
BclI BlnI BsaHI BseAI BsiWI
BsmBI BspDI BspEI BsrBI BssHII
BstBI BstUI BstXI ClaI CpoI
DraIII DrdI EagI EclXI Eco52I
EcoRI FbaI Hin1I KasI KspI
MluI MroI MvnI NarI NheI
NotI NruI NspV PacI PinAI
PmeI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 3952 GGTACC G^GTACC
AclI 2501 AACGTT AA^CGTT
AhdI 3120 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4743 ATTAAT AT^TAAT
AsnI 4743 ATTAAT AT^TAAT
Asp718I 3952 GGTACC G^GTACC
AspEI 3120 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1051 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 190 TGCGCA TGC^GCA
BsaBI 5269 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2980 ACCGGA [AT]^CCGG[AT]
BsiEI 2978 CGACCG CG[GA][CT]^CG
BspHI 2735 TCATGA T^CATGA
BssSI 2057 CACGAG CACGAG(-5/-1)
Bst1107I 4423 GTATAC GTA^TAC
BstEII 1493 GGTCACC G^GT[ACGT]ACC
BstPI 1493 GGTCACC G^GT[ACGT]ACC
BstZ17I 4423 GTATAC GTA^TAC
Eam1105I 3120 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1493 GGTCACC G^GT[ACGT]ACC
EcoRV 2329 GATATC GAT^ATC
FauI 4635 CCCGC CCCGC(4/6)
FseI 1273 GGCCGGCC GGCCGG^CC
FspI 190 TGCGCA TGC^GCA
KpnI 3952 GGTACC GGTAC^C
MamI 5269 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1274 GCCGGC GCC^GGC
NcoI 3500 CCATGG C^CATGG
NgoMIV 1274 GCCGGC G^CCGGC
PaeR7I 8 CTCGAG C^TCGAG
PflFI 1051 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 318 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3558 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4743 ATTAAT AT^TAAT
Psp1406I 2501 AACGTT AA^CGTT
RcaI 2735 TCATGA T^CATGA
SphI 897 GCATGC GCATG^C
Tth111I 1051 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 8 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Aor51HI 3551 AGCGCT AGC^GCT
3675 AGCGCT
ApaI 1871 GGGCCC GGGCC^C
3934 GGGCCC
AspHI 2862 GAGCAC G[AT]GC[AT]^C
4365 GTGCTC
AvaI 8 CTCGAG C^[CT]CG[GA]G
2162 CTCGGG
BalI 3312 TGGCCA TGG^CCA
5840 TGGCCA
BamHI 2040 GGATCC G^GATCC
2876 GGATCC
BanI 1906 GGCACC G^G[CT][GA]CC
3952 GGTACC
BbrPI 1554 CACGTG CAC^GTG
3123 CACGTG
BciVI 1411 GGATAC GTATCC(6/5)
5031 GGATAC
BseRI 2071 CTCCTC GAGGAG(10/8)
1661 GAGGAG
BsgI 104 CTGCAC GTGCAG(16/14)
2832 GTGCAG
BsiHKAI 2862 GAGCAC G[AT]GC[AT]^C
4365 GTGCTC
BsoBI 8 CTCGAG C^[CT]CG[GA]G
2162 CTCGGG
BsrFI 1274 GCCGGC [GA]^CCGG[CT]
5439 GCCGGT
Cfr10I 1274 GCCGGC [GA]^CCGG[CT]
5439 GCCGGT
Eco47III 3551 AGCGCT AGC^GCT
3675 AGCGCT
EcoNI 2280 CCTTTCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3525 CCTATGCAAGG
HaeII 3551 AGCGCT [GA]GCGC^[CT]
3675 AGCGCT
HpaI 3853 GTTAAC GTT^AAC
6084 GTTAAC
MfeI 481 CAATTG C^AATTG
5343 CAATTG
MluNI 3312 TGGCCA TGG^CCA
5840 TGGCCA
MscI 3312 TGGCCA TGG^CCA
5840 TGGCCA
MunI 481 CAATTG C^AATTG
5343 CAATTG
PmaCI 1554 CACGTG CAC^GTG
3123 CACGTG
PmlI 1554 CACGTG CAC^GTG
3123 CACGTG
SapI 472 GAAGAGC GCTCTTC(1/4)
3516 GAAGAGC
SspI 4099 AATATT AAT^ATT
5797 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AvaII BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BfrI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspLU11I BspMI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI