################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII AclI AcsI AflII AgeI ApoI AscI AseI AsnI AvrII BclI BfrI BlnI BsiEI BsiWI BspDI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoRI EcoT22I FbaI FseI HincII HindII HpaI MfeI MluI MunI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SfuI SgrAI SnaBI SpeI SphI SspI SwaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AccIII 4698 TCCGGA T^CCGGA AocI 3395 CCTGAGG CC^T[ACGT]AGG AviII 1305 TGCGCA TGC^GCA BbrPI 605 CACGTG CAC^GTG BcgI 1404 GCATGGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 4321 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 4698 TCCGGA T^CCGGA BspEI 4698 TCCGGA T^CCGGA BspLU11I 656 ACATGT A^CATGT BssHII 89 GCGCGC G^CGCGC Bsu36I 3395 CCTGAGG CC^T[ACGT]AGG Eco81I 3395 CCTGAGG CC^T[ACGT]AGG EcoRV 4345 GATATC GAT^ATC FspI 1305 TGCGCA TGC^GCA HindIII 3176 AAGCTT A^AGCTT KspI 4537 CCGCGG CCGC^GG MamI 4321 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 4698 TCCGGA T^CCGGA MseI 3603 TTAA T^TAA NheI 4030 GCTAGC G^CTAGC PmaCI 605 CACGTG CAC^GTG PmlI 605 CACGTG CAC^GTG SacII 4537 CCGCGG CCGC^GG ScaI 4052 AGTACT AGT^ACT SexAI 4611 ACCAGGT A^CC[AT]GGT SfiI 5807 GGCCGCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Tru9I 3603 TTAA T^TAA XbaI 6048 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 740 GTAGAC GT^[AC][GT]AC 4808 GTAGAC AflIII 656 ACATGT A^C[GA][CT]GT 5654 ACACGT AhdI 2688 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5650 GACAACACGTC Asp700I 3706 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5629 GAAGGCCTTC AspEI 2688 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5650 GACAACACGTC BamHI 1156 GGATCC G^GATCC 5715 GGATCC BciVI 3686 GTATCC GTATCC(6/5) 4433 GTATCC BglII 3414 AGATCT A^GATCT 4042 AGATCT BsaWI 4698 TCCGGA [AT]^CCGG[AT] 5712 ACCGGA BsmBI 2591 GAGACG CGTCTC(1/5) 4931 GAGACG BspHI 1480 TCATGA T^CATGA 5460 TCATGA DrdI 713 GACCGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2003 GACTTTGTCGTC Eam1105I 2688 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5650 GACAACACGTC NdeI 3619 CATATG CA^TATG 4851 CATATG PshAI 3266 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5057 GACAGCCGTC RcaI 1480 TCATGA T^CATGA 5460 TCATGA SapI 778 GCTCTTC GCTCTTC(1/4) 3154 GCTCTTC SbfI 2032 CCTGCAGG CCTGCA^GG 3871 CCTGCAGG SmaI 42 CCCGGG CCC^GGG 5759 CCCGGG Sse8387I 2032 CCTGCAGG CCTGCA^GG 3871 CCTGCAGG StuI 613 AGGCCT AGG^CCT 5631 AGGCCT TfiI 319 GAATC G^A[AT]TC 4082 GATTC XmaCI 42 CCCGGG C^CCGGG 5759 CCCGGG XmaI 42 CCCGGG C^CCGGG 5759 CCCGGG XmnI 3706 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5629 GAAGGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 150 enzymes) # ######################################### Acc65I AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI Asp718I AspHI AspI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PaeR7I PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspBI StyI TaqI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoI XhoII