##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
ApoI AscI AseI AsnI AvrII
BclI BfrI BlnI BsiEI BsiWI
BspDI BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI EclXI
Eco52I EcoRI EcoT22I FbaI FseI
HincII HindII HpaI MfeI MluI
MunI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 4698 TCCGGA T^CCGGA
AocI 3395 CCTGAGG CC^T[ACGT]AGG
AviII 1305 TGCGCA TGC^GCA
BbrPI 605 CACGTG CAC^GTG
BcgI 1404 GCATGGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 4321 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4698 TCCGGA T^CCGGA
BspEI 4698 TCCGGA T^CCGGA
BspLU11I 656 ACATGT A^CATGT
BssHII 89 GCGCGC G^CGCGC
Bsu36I 3395 CCTGAGG CC^T[ACGT]AGG
Eco81I 3395 CCTGAGG CC^T[ACGT]AGG
EcoRV 4345 GATATC GAT^ATC
FspI 1305 TGCGCA TGC^GCA
HindIII 3176 AAGCTT A^AGCTT
KspI 4537 CCGCGG CCGC^GG
MamI 4321 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4698 TCCGGA T^CCGGA
MseI 3603 TTAA T^TAA
NheI 4030 GCTAGC G^CTAGC
PmaCI 605 CACGTG CAC^GTG
PmlI 605 CACGTG CAC^GTG
SacII 4537 CCGCGG CCGC^GG
ScaI 4052 AGTACT AGT^ACT
SexAI 4611 ACCAGGT A^CC[AT]GGT
SfiI 5807 GGCCGCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I 3603 TTAA T^TAA
XbaI 6048 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 740 GTAGAC GT^[AC][GT]AC
4808 GTAGAC
AflIII 656 ACATGT A^C[GA][CT]GT
5654 ACACGT
AhdI 2688 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5650 GACAACACGTC
Asp700I 3706 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5629 GAAGGCCTTC
AspEI 2688 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5650 GACAACACGTC
BamHI 1156 GGATCC G^GATCC
5715 GGATCC
BciVI 3686 GTATCC GTATCC(6/5)
4433 GTATCC
BglII 3414 AGATCT A^GATCT
4042 AGATCT
BsaWI 4698 TCCGGA [AT]^CCGG[AT]
5712 ACCGGA
BsmBI 2591 GAGACG CGTCTC(1/5)
4931 GAGACG
BspHI 1480 TCATGA T^CATGA
5460 TCATGA
DrdI 713 GACCGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2003 GACTTTGTCGTC
Eam1105I 2688 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5650 GACAACACGTC
NdeI 3619 CATATG CA^TATG
4851 CATATG
PshAI 3266 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5057 GACAGCCGTC
RcaI 1480 TCATGA T^CATGA
5460 TCATGA
SapI 778 GCTCTTC GCTCTTC(1/4)
3154 GCTCTTC
SbfI 2032 CCTGCAGG CCTGCA^GG
3871 CCTGCAGG
SmaI 42 CCCGGG CCC^GGG
5759 CCCGGG
Sse8387I 2032 CCTGCAGG CCTGCA^GG
3871 CCTGCAGG
StuI 613 AGGCCT AGG^CCT
5631 AGGCCT
TfiI 319 GAATC G^A[AT]TC
4082 GATTC
XmaCI 42 CCCGGG C^CCGGG
5759 CCCGGG
XmaI 42 CCCGGG C^CCGGG
5759 CCCGGG
XmnI 3706 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5629 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI Asp718I AspHI AspI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PaeR7I PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspBI StyI TaqI
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoI XhoII