################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII AccIII AgeI Aor51HI AscI AvaI AviII BamHI BcgI BciVI BsaI BseAI BsgI BsiWI BsoBI BspDI BspEI BsrBI BssHII BssSI BstAPI BstBI BstXI ClaI CpoI Eco47III FseI FspI MluI MroI NruI NspV PacI PaeR7I PinAI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 996 GGTACC G^GTACC AccI 2794 GTATAC GT^[AC][GT]AC AclI 4616 AACGTT AA^CGTT AhdI 5042 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2997 GTGCAC G^TGCAC ApaLI 2997 GTGCAC G^TGCAC AseI 2285 ATTAAT AT^TAAT AsnI 2285 ATTAAT AT^TAAT Asp718I 996 GGTACC G^GTACC AspEI 5042 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 2106 CCTAGG C^CTAGG BalI 2203 TGGCCA TGG^CCA BbeI 33 GGCGCC GGCGC^C BbrPI 2966 CACGTG CAC^GTG BbvCI 10 GCTGAGG CCTCAGC(-5/-2) BlnI 2106 CCTAGG C^CTAGG BlpI 2698 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2698 GCTGAGC GC^T[ACGT]AGC BsaAI 2966 CACGTG [CT]AC^GT[GA] BsaBI 1589 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4164 ACCGGA [AT]^CCGG[AT] BspHI 2684 TCATGA T^CATGA BsrDI 1437 CATTGC GCAATG(2/0) Bst1107I 2794 GTATAC GTA^TAC BstEII 754 GGTCACC G^GT[ACGT]ACC BstPI 754 GGTCACC G^GT[ACGT]ACC BstZ17I 2794 GTATAC GTA^TAC CelII 2698 GCTGAGC GC^T[ACGT]AGC DraIII 1614 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 5042 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 754 GGTCACC G^GT[ACGT]ACC HaeII 33 GGCGCC [GA]GCGC^[CT] HindIII 4116 AAGCTT A^AGCTT KasI 33 GGCGCC G^GCGCC KpnI 996 GGTACC GGTAC^C MamI 1589 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 560 CAATTG C^AATTG MluNI 2203 TGGCCA TGG^CCA MscI 2203 TGGCCA TGG^CCA MunI 560 CAATTG C^AATTG NaeI 920 GCCGGC GCC^GGC NarI 33 GGCGCC GG^CGCC NgoMIV 920 GCCGGC G^CCGGC NheI 1514 GCTAGC G^CTAGC NotI 286 GCGGCCGC GC^GGCCGC PmaCI 2966 CACGTG CAC^GTG PmeI 4594 GTTTAAAC GTTT^AAAC PmlI 2966 CACGTG CAC^GTG PshAI 2626 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2285 ATTAAT AT^TAAT Psp1406I 4616 AACGTT AA^CGTT PstI 4275 CTGCAG CTGCA^G PvuI 3268 CGATCG CGAT^CG RcaI 2684 TCATGA T^CATGA SfoI 33 GGCGCC GGC^GCC StuI 429 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 33 GGCGCC G[GA]^CG[CT]C 3630 GACGCC AflII 4154 CTTAAG C^TTAAG 5278 CTTAAG AocI 1328 CCTGAGG CC^T[ACGT]AGG 3816 CCTAAGG ApaI 280 GGGCCC GGGCC^C 1839 GGGCCC AspI 3908 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4099 GACTCTGTC BclI 2377 TGATCA T^GATCA 2444 TGATCA BfrI 4154 CTTAAG C^TTAAG 5278 CTTAAG BsaHI 33 GGCGCC G[GA]^CG[CT]C 3630 GACGCC BsmBI 888 CGTCTC CGTCTC(1/5) 91 GAGACG BsmI 989 GAATGC GAATGC(1/-1) 5148 GAATGC BsrFI 920 GCCGGC [GA]^CCGG[CT] 993 GCCGGT Bsu36I 1328 CCTGAGG CC^T[ACGT]AGG 3816 CCTAAGG Cfr10I 920 GCCGGC [GA]^CCGG[CT] 993 GCCGGT DrdI 1636 GACATATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3022 GACACTAGAGTC Eco81I 1328 CCTGAGG CC^T[ACGT]AGG 3816 CCTAAGG EcoRI 3327 GAATTC G^AATTC 5029 GAATTC FbaI 2377 TGATCA T^GATCA 2444 TGATCA Hin1I 33 GGCGCC G[GA]^CG[CT]C 3630 GACGCC HpaI 3095 GTTAAC GTT^AAC 4216 GTTAAC NcoI 1388 CCATGG C^CATGG 3017 CCATGG PflFI 3908 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4099 GACTCTGTC PpuMI 3893 GGGTCCT [GA]G^G[AT]CC[CT] 4773 AGGTCCT SspI 2428 AATATT AAT^ATT 2550 AATATT Tth111I 3908 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4099 GACTCTGTC XbaI 1777 TCTAGA T^CTAGA 5015 TCTAGA XcmI 2206 CCATTTACTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3077 CCATATGGGTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BspMI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoRV EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI