##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccIII AgeI Aor51HI AscI
AvaI AviII BamHI BcgI BciVI
BsaI BseAI BsgI BsiWI BsoBI
BspDI BspEI BsrBI BssHII BssSI
BstAPI BstBI BstXI ClaI CpoI
Eco47III FseI FspI MluI MroI
NruI NspV PacI PaeR7I PinAI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 996 GGTACC G^GTACC
AccI 2794 GTATAC GT^[AC][GT]AC
AclI 4616 AACGTT AA^CGTT
AhdI 5042 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2997 GTGCAC G^TGCAC
ApaLI 2997 GTGCAC G^TGCAC
AseI 2285 ATTAAT AT^TAAT
AsnI 2285 ATTAAT AT^TAAT
Asp718I 996 GGTACC G^GTACC
AspEI 5042 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2106 CCTAGG C^CTAGG
BalI 2203 TGGCCA TGG^CCA
BbeI 33 GGCGCC GGCGC^C
BbrPI 2966 CACGTG CAC^GTG
BbvCI 10 GCTGAGG CCTCAGC(-5/-2)
BlnI 2106 CCTAGG C^CTAGG
BlpI 2698 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2698 GCTGAGC GC^T[ACGT]AGC
BsaAI 2966 CACGTG [CT]AC^GT[GA]
BsaBI 1589 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4164 ACCGGA [AT]^CCGG[AT]
BspHI 2684 TCATGA T^CATGA
BsrDI 1437 CATTGC GCAATG(2/0)
Bst1107I 2794 GTATAC GTA^TAC
BstEII 754 GGTCACC G^GT[ACGT]ACC
BstPI 754 GGTCACC G^GT[ACGT]ACC
BstZ17I 2794 GTATAC GTA^TAC
CelII 2698 GCTGAGC GC^T[ACGT]AGC
DraIII 1614 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5042 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 754 GGTCACC G^GT[ACGT]ACC
HaeII 33 GGCGCC [GA]GCGC^[CT]
HindIII 4116 AAGCTT A^AGCTT
KasI 33 GGCGCC G^GCGCC
KpnI 996 GGTACC GGTAC^C
MamI 1589 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 560 CAATTG C^AATTG
MluNI 2203 TGGCCA TGG^CCA
MscI 2203 TGGCCA TGG^CCA
MunI 560 CAATTG C^AATTG
NaeI 920 GCCGGC GCC^GGC
NarI 33 GGCGCC GG^CGCC
NgoMIV 920 GCCGGC G^CCGGC
NheI 1514 GCTAGC G^CTAGC
NotI 286 GCGGCCGC GC^GGCCGC
PmaCI 2966 CACGTG CAC^GTG
PmeI 4594 GTTTAAAC GTTT^AAAC
PmlI 2966 CACGTG CAC^GTG
PshAI 2626 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2285 ATTAAT AT^TAAT
Psp1406I 4616 AACGTT AA^CGTT
PstI 4275 CTGCAG CTGCA^G
PvuI 3268 CGATCG CGAT^CG
RcaI 2684 TCATGA T^CATGA
SfoI 33 GGCGCC GGC^GCC
StuI 429 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 33 GGCGCC G[GA]^CG[CT]C
3630 GACGCC
AflII 4154 CTTAAG C^TTAAG
5278 CTTAAG
AocI 1328 CCTGAGG CC^T[ACGT]AGG
3816 CCTAAGG
ApaI 280 GGGCCC GGGCC^C
1839 GGGCCC
AspI 3908 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4099 GACTCTGTC
BclI 2377 TGATCA T^GATCA
2444 TGATCA
BfrI 4154 CTTAAG C^TTAAG
5278 CTTAAG
BsaHI 33 GGCGCC G[GA]^CG[CT]C
3630 GACGCC
BsmBI 888 CGTCTC CGTCTC(1/5)
91 GAGACG
BsmI 989 GAATGC GAATGC(1/-1)
5148 GAATGC
BsrFI 920 GCCGGC [GA]^CCGG[CT]
993 GCCGGT
Bsu36I 1328 CCTGAGG CC^T[ACGT]AGG
3816 CCTAAGG
Cfr10I 920 GCCGGC [GA]^CCGG[CT]
993 GCCGGT
DrdI 1636 GACATATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3022 GACACTAGAGTC
Eco81I 1328 CCTGAGG CC^T[ACGT]AGG
3816 CCTAAGG
EcoRI 3327 GAATTC G^AATTC
5029 GAATTC
FbaI 2377 TGATCA T^GATCA
2444 TGATCA
Hin1I 33 GGCGCC G[GA]^CG[CT]C
3630 GACGCC
HpaI 3095 GTTAAC GTT^AAC
4216 GTTAAC
NcoI 1388 CCATGG C^CATGG
3017 CCATGG
PflFI 3908 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4099 GACTCTGTC
PpuMI 3893 GGGTCCT [GA]G^G[AT]CC[CT]
4773 AGGTCCT
SspI 2428 AATATT AAT^ATT
2550 AATATT
Tth111I 3908 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4099 GACTCTGTC
XbaI 1777 TCTAGA T^CTAGA
5015 TCTAGA
XcmI 2206 CCATTTACTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3077 CCATATGGGTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspLU11I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoRV EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI