##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AviII BclI BfrI
BsiWI BsmBI BspDI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI Eam1105I
Eco47III EcoRV FbaI FseI FspI
HgaI HindIII MfeI MluI MunI
NruI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SalI ScaI SfiI SfuI SgrAI
SmaI SnaBI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 1098 TCCGGA T^CCGGA
AcyI 45 GGCGCC G[GA]^CG[CT]C
BamHI 4618 GGATCC G^GATCC
BbeI 45 GGCGCC GGCGC^C
BbrPI 6179 CACGTG CAC^GTG
BcgI 4162 GCATGAAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 5968 AGATCT A^GATCT
BsaBI 840 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 45 GGCGCC G[GA]^CG[CT]C
BseAI 1098 TCCGGA T^CCGGA
BsiEI 10 CGGCCG CG[GA][CT]^CG
BsmI 5078 GCATTC GAATGC(1/-1)
BspEI 1098 TCCGGA T^CCGGA
BspHI 4263 TCATGA T^CATGA
BspMI 1596 ACCTGC ACCTGC(4/8)
BssHII 64 GCGCGC G^CGCGC
BstXI 3040 CCATCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2046 TTTAAA TTT^AAA
EagI 10 CGGCCG C^GGCCG
EclXI 10 CGGCCG C^GGCCG
Eco52I 10 CGGCCG C^GGCCG
EcoRI 1968 GAATTC G^AATTC
EcoT22I 4147 ATGCAT ATGCA^T
Hin1I 45 GGCGCC G[GA]^CG[CT]C
HincII 6333 GTTAAC GT[CT]^[GA]AC
HindII 6333 GTTAAC GT[CT]^[GA]AC
HpaI 6333 GTTAAC GTT^AAC
KasI 45 GGCGCC G^GCGCC
KspI 1278 CCGCGG CCGC^GG
MamI 840 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1098 TCCGGA T^CCGGA
NaeI 152 GCCGGC GCC^GGC
NarI 45 GGCGCC GG^CGCC
NdeI 3404 CATATG CA^TATG
NgoMIV 152 GCCGGC G^CCGGC
NotI 9 GCGGCCGC GC^GGCCGC
NsiI 4147 ATGCAT ATGCA^T
PaeR7I 4651 CTCGAG C^TCGAG
PmaCI 6179 CACGTG CAC^GTG
PmlI 6179 CACGTG CAC^GTG
RcaI 4263 TCATGA T^CATGA
SacII 1278 CCGCGG CCGC^GG
SbfI 446 CCTGCAGG CCTGCA^GG
SfoI 45 GGCGCC GGC^GCC
SphI 4149 GCATGC GCATG^C
Sse8387I 446 CCTGCAGG CCTGCA^GG
XhoI 4651 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 778 GGTACC G^GTACC
2677 GGTACC
AccI 1958 GTCTAC GT^[AC][GT]AC
4695 GTAGAC
AflIII 2588 ACATGT A^C[GA][CT]GT
5062 ACATGT
ApaI 1250 GGGCCC GGGCC^C
1480 GGGCCC
Asp718I 778 GGTACC G^GTACC
2677 GGTACC
AvaI 1569 CCCGAG C^[CT]CG[GA]G
4651 CTCGAG
BsaAI 3551 TACGTG [CT]AC^GT[GA]
6179 CACGTG
BsoBI 1569 CCCGAG C^[CT]CG[GA]G
4651 CTCGAG
BspLU11I 2588 ACATGT A^CATGT
5062 ACATGT
BsrBI 673 CCGCTC CCGCTC(-3/-3)
423 GAGCGG
BsrFI 152 GCCGGC [GA]^CCGG[CT]
913 ACCGGC
BstEII 1357 GGTAACC G^GT[ACGT]ACC
1519 GGTTACC
BstPI 1357 GGTAACC G^GT[ACGT]ACC
1519 GGTTACC
Cfr10I 152 GCCGGC [GA]^CCGG[CT]
913 ACCGGC
DrdI 5819 GACAGTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6214 GACTTCTATGTC
EcoNI 170 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
304 CCTCTCTGAGG
EcoO65I 1357 GGTAACC G^GT[ACGT]ACC
1519 GGTTACC
KpnI 778 GGTACC GGTAC^C
2677 GGTACC
NheI 188 GCTAGC G^CTAGC
4634 GCTAGC
SexAI 370 ACCAGGT A^CC[AT]GGT
4723 ACCAGGT
SspI 3518 AATATT AAT^ATT
4340 AATATT
TaqI 1593 TCGA T^CGA
4652 TCGA
TthHB8I 1593 TCGA T^CGA
4652 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AspI AvaII AvrII BalI
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PshAI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI SspBI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoII XmnI