##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccII AccIII AclI ApaI
AscI AspI AvaI AviII BamHI
BbeI BseAI BsiEI BsiWI BsoBI
Bsp1407I BspEI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
CpoI EagI EclXI Eco52I EcoRV
FseI FspI HpaI KasI KspI
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PmeI
PshAI Psp1406I PvuI RsrII SacII
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI StuI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1357 GGTACC G^GTACC
AflII 4338 CTTAAG C^TTAAG
AgeI 118 ACCGGT A^CCGGT
Alw44I 3812 GTGCAC G^TGCAC
AocI 1545 CCTCAGG CC^T[ACGT]AGG
Aor51HI 235 AGCGCT AGC^GCT
ApaLI 3812 GTGCAC G^TGCAC
Asp718I 1357 GGTACC G^GTACC
AvrII 3001 CCTAGG C^CTAGG
BbvCI 3801 CCTCAGC CCTCAGC(-5/-2)
BfrI 4338 CTTAAG C^TTAAG
BglII 3337 AGATCT A^GATCT
BlnI 3001 CCTAGG C^CTAGG
BlpI 4795 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4795 GCTTAGC GC^T[ACGT]AGC
BsaBI 4588 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2257 CGTCTC CGTCTC(1/5)
BsrBI 3013 CCGCTC CCGCTC(-3/-3)
BsrFI 118 ACCGGT [GA]^CCGG[CT]
Bsu36I 1545 CCTCAGG CC^T[ACGT]AGG
CelII 4795 GCTTAGC GC^T[ACGT]AGC
Cfr10I 118 ACCGGT [GA]^CCGG[CT]
Eco47III 235 AGCGCT AGC^GCT
Eco81I 1545 CCTCAGG CC^T[ACGT]AGG
EcoNI 1591 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 1828 AAGCTT A^AGCTT
KpnI 1357 GGTACC GGTAC^C
MamI 4588 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6133 CAATTG C^AATTG
MunI 6133 CAATTG C^AATTG
PinAI 118 ACCGGT A^CCGGT
SacI 2747 GAGCTC GAGCT^C
SalI 4634 GTCGAC G^TCGAC
SpeI 4661 ACTAGT A^CTAGT
XbaI 5416 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 1496 GGCGTC G[GA]^CG[CT]C
6675 GACGCC
BbrPI 3809 CACGTG CAC^GTG
4849 CACGTG
BclI 1018 TGATCA T^GATCA
5393 TGATCA
BglI 746 GCCTACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2988 GCCAGCAGGGC
BsaAI 3809 CACGTG [CT]AC^GT[GA]
4849 CACGTG
BsaHI 1496 GGCGTC G[GA]^CG[CT]C
6675 GACGCC
BspDI 795 ATCGAT AT^CGAT
1622 ATCGAT
BspLU11I 1851 ACATGT A^CATGT
4059 ACATGT
BstAPI 659 GCAGTAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4124 GCAGATGCTGC
ClaI 795 ATCGAT AT^CGAT
1622 ATCGAT
DraIII 779 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2314 CACCTGGTG
EcoRI 2373 GAATTC G^AATTC
3304 GAATTC
FbaI 1018 TGATCA T^GATCA
5393 TGATCA
Hin1I 1496 GGCGTC G[GA]^CG[CT]C
6675 GACGCC
PflMI 2506 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4697 CCATAAAATGG
PmaCI 3809 CACGTG CAC^GTG
4849 CACGTG
PmlI 3809 CACGTG CAC^GTG
4849 CACGTG
PpuMI 1377 AGGACCT [GA]G^G[AT]CC[CT]
2560 GGGTCCC
PvuII 2963 CAGCTG CAG^CTG
5267 CAGCTG
SexAI 379 ACCTGGT A^CC[AT]GGT
2315 ACCTGGT
Van91I 2506 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4697 CCATAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspEI AspHI
AvaII BalI BanI BanII BbsI
BbvI BcgI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstEII BstF5I BstNI BstPI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
Eam1105I EarI EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI PstI RcaI RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI